Diversity and genetic characterization of orthohantavirus from small mammals and humans during 2012–2022 in Hubei Province, Central China

Chen, Jin-tao; Zhan, Jian-bo; Zhu, Ming-chao; Li, Kai-ji; Liu, Man-qing; Hu, Bin; Cai, Kun; Xiong, Hai-rong; Chen, Shu-liang; Tan, Wei-long; Chen, Liang-jun; Hou, Wei

doi:10.1016/j.actatropica.2023.107046

“…There exist numerous branches of HTNV, with highly diverse genetics in Heilongjiang, Shanxi, Liaoning, Shandong, Jilin, Hebei, Hunan, Zhejiang, Jiangxi, Jiangsu and Hubei provinces (33). Recently, the genetic evolution analysis of the L segment revealed that the viral sequences prevalent in Hubei province cluster together, forming a distinct lineage with genetic variations from viruses in other regions (18). To enhance our detection capabilities, we have designed two sets of primer and probe for HTNV infection.…”

Section: Pcr Efficiency Lod and Precision Of All-in-one Real-time Pcr...mentioning

confidence: 99%

“…The main clinical symptoms of tick-borne rickettsioses also include fever and thrombocytopenia as well as headache, muscle pain, rash and local lymphadenopathy (13)(14)(15)(16) (12,17). It is worth noting that central China including the Dabie and Yiling Mountains region is also known to be an important endemic area for orthohantavirus hantanense (HTNV) infections transmitted by rodents, which belongs to the order Bunyavirales, family Hantaviridae, and genus Orthohantavirus (18). The main epidemic strain of HTNV is HV004 in the past ten years (18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

“…It is worth noting that central China including the Dabie and Yiling Mountains region is also known to be an important endemic area for orthohantavirus hantanense (HTNV) infections transmitted by rodents, which belongs to the order Bunyavirales, family Hantaviridae, and genus Orthohantavirus (18). The main epidemic strain of HTNV is HV004 in the past ten years (18)(19)(20). Hemorrhagic fever with renal syndrome (HFRS), caused by HTNV, is characterized by a combination of symptoms, which include fever, hemorrhage, thrombocytopenia and acute kidney injury (21,22).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of an all-in-one real-time PCR assay for simultaneous detection of spotted fever group rickettsiae, severe fever with thrombocytopenia syndrome virus and orthohantavirus hantanense prevalent in central China

Wang,

Chen,

Li

et al. 2024

Preprint

0

View full text Add to dashboard Cite

Central China has been reported to be one of the most important endemic areas of zoonotic infection by spotted fever group rickettsiae(SFGR), severe fever with thrombocytopenia syndrome virus (SFTSV) and orthohantavirus hantanense(HTNV). Due to similar clinical symptoms, it is challenging to make a definite diagnosis rapidly and accurately in the absence of microbiological tests. In the present study, an all-in-one real-time PCR assay was developed for the simultaneous detection of nucleic acids from SFGR, SFTSV and HTNV. Three linear standard curves for determining SFGR-ompA, SFTSV-Land HTNV-Lwere obtained within the range of 101-106copies/μL, with the PCR amplification efficiencies ranging from 93.46% to 96.88% and the regression coefficients R2of >0.99. The detection limit was 1.108 copies/μL for SFGR-ompA, 1.075 copies/μL for SFTSV-Land 1.006 copies/μL for HTNV-L, respectively. Both the within-run and within-laboratory coefficients of variation on the cycle threshold (Ct) values were within the range of 0.53%-2.15%. It was also found there was no statistical difference in the Ct values between with and without other non-target bloodborne virus nucleic acids (PSFGR-ompA=0.186, PSFTSV-L=0.612, PHTNV-L=0.298). The sensitivity, specificity, positive and negative predictive value were all 100% for determining SFGR-ompAand SFTSV-L, 97%, 100%, 100% and 99.6% for HTNV-L, respectively. Therefore, the all-in-one real-time PCR assay appears to be a reliable, sensitive, rapid, high-throughput and low cost-effective method to diagnose the zoonotic infection by SFGR, SFTSV and HTNV.Author SummarySpotted fever, severe fever with thrombocytopenia syndrome (SFTS), and hemorrhagic fever with renal syndrome (HFRS) sporadically have outbreaks in central China. Due to the similarities in clinical symptoms and the absence of reliable diagnostic methods, clinical diagnosis and treatment frequently result in misdiagnosis or missed diagnosis. Thus, the development of a fast and accurate diagnostic method is crucial for prevention and precise treatment. In this study, we designed an all-in-one real-time PCR assay to differentiate spotted fever group rickettsiae(SFGR), severe fever with thrombocytopenia syndrome virus (SFTSV) and orthohantavirus hantanense(HTNV). The geneompAof SFGR, as well as the gene segmentLof SFTSV and HTNV, were used as targets to design primers and probes for amplification. Through the verification of nucleic acid and clinical sample detection, the sensitivity of this detection method exceeded 97%, and its specificity was 100%.This new assay could be applied in epidemiology and clinical diagnosis, to control new outbreaks, reduce diagnostic and identification time, and improve test efficiency.

show abstract

Hantavirus Pulmonary Syndrome in Northwestern Argentina: Seroprevalence in rodents of Jujuy province and first seropositive record for Euryoryzomys legatus

Cassinelli,

López,

Coelho

et al. 2025

Acta Tropica

0

View full text Add to dashboard Cite

Development of an all-in-one real-time PCR assay for simultaneous detection of spotted fever group rickettsiae, severe fever with thrombocytopenia syndrome virus and hantaan virus prevalent in central China

Wang,

Chen,

Li

et al. 2024

PLoS Negl Trop Dis

0

View full text Add to dashboard Cite

Central China has been reported to be one of the most important endemic areas of zoonotic infection by spotted fever group rickettsiae (SFGR), severe fever with thrombocytopenia syndrome virus (SFTSV) and hantaan virus (HTNV). Due to similar clinical symptoms, it is challenging to make a definite diagnosis rapidly and accurately in the absence of microbiological tests. In the present study, an all-in-one real-time PCR assay was developed for the simultaneous detection of nucleic acids from SFGR, SFTSV and HTNV. Three linear standard curves for determining SFGR-ompA, SFTSV-L and HTNV-L were obtained within the range of 101−106 copies/μL, with the PCR amplification efficiencies ranging from 93.46% to 96.88% and the regression coefficients R2 of >0.99. The detection limit was 1.108 copies/μL for SFGR-ompA, 1.075 copies/μL for SFTSV-L and 1.006 copies/μL for HTNV-L, respectively. Both the within-run and within-laboratory coefficients of variation on the cycle threshold (Ct) values were within the range of 0.53%-2.15%. It was also found there was no statistical difference in the Ct values between single template and multiple templates (PSFGR-ompA = 0.186, PSFTSV-L = 0.612, PHTNV-L = 0.298). The sensitivity, specificity, positive and negative predictive value were all 100% for determining SFGR-ompA and SFTSV-L, 97%, 100%, 100% and 99.6% for HTNV-L, respectively. Therefore, the all-in-one real-time PCR assay appears to be a reliable, sensitive, rapid, high-throughput and low cost-effective method to diagnose the zoonotic infection by SFGR, SFTSV and HTNV.

show abstract

Diversity and genetic characterization of orthohantavirus from small mammals and humans during 2012–2022 in Hubei Province, Central China

Cited by 3 publications

References 42 publications

Development of an all-in-one real-time PCR assay for simultaneous detection of spotted fever group rickettsiae, severe fever with thrombocytopenia syndrome virus and orthohantavirus hantanense prevalent in central China

Development of an all-in-one real-time PCR assay for simultaneous detection of spotted fever group rickettsiae, severe fever with thrombocytopenia syndrome virus and orthohantavirus hantanense prevalent in central China

Hantavirus Pulmonary Syndrome in Northwestern Argentina: Seroprevalence in rodents of Jujuy province and first seropositive record for Euryoryzomys legatus

Development of an all-in-one real-time PCR assay for simultaneous detection of spotted fever group rickettsiae, severe fever with thrombocytopenia syndrome virus and hantaan virus prevalent in central China

Contact Info

Product

Resources

About