Diversity and metabolism of xylose and glucose fermenting microbial communities in sequencing batch or continuous culturing

Rombouts, Julius Laurens; Mos, Galvin; Weissbrodt, David G.; Kleerebezem, Robbert; Loosdrecht, Mark C.M. van

doi:10.1093/femsec/fiy233

Cited by 34 publications

(49 citation statements)

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“…Volatile fatty acids (VFAs; formate to valerate), lactate, succinate and glucose were analysed using high‐performance liquid chromatography (HPLC) method described previously (Rombouts, Mos, Weissbrodt, Kleerebezem, & van Loosdrecht, 2019). Ethanol was analysed using a gas chromatography (GC) method described previously (Rombouts et al, 2019). The off‐gases were monitored on‐line for H 2 and CO 2 by a connection to a NGA 2000 MLT 1 multicomponent analyser (Rosemount, Shakopee, MN).…”

Section: Methodsmentioning

confidence: 99%

“…This script is based on determining the last moment of base dosage in a cycle, which indicates the end of the fermentation, and thus the growth. This method is explained in detail in the supplementary information section published previously (Rombouts et al, 2019). The maximum biomass‐specific substrate conversion rate ( q s max ) is calculated using µ max and Y x ,s using the Herbert‐Pirt equation (Pirt, 1965) and neglecting maintenance.…”

Section: Methodsmentioning

confidence: 99%

“…Samples from the reactors were immediately filtered on 0.45 µm polyvinylidene fluoride membranes (Millipore) and stored at −20°C until analysis. Volatile fatty acids (VFAs; formate to valerate), lactate, succinate and glucose were analysed using high-performance liquid chromatography (HPLC) method described previously (Rombouts, Mos, Weissbrodt, Kleerebezem, & van Loosdrecht, 2019). Ethanol was analysed using a gas chromatography (GC) method described previously (Rombouts et al, 2019 200°C) with N 2 as a carrier gas (2 ml min −1 ).…”

Section: Methodsmentioning

confidence: 99%

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Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures

Rombouts

Kranendonk

Regueira

et al. 2020

Biotech & Bioengineering

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Lactic acid‐producing bacteria are important in many fermentations, such as the production of biobased plastics. Insight in the competitive advantage of lactic acid bacteria over other fermentative bacteria in a mixed culture enables ecology‐based process design and can aid the development of sustainable and energy‐efficient bioprocesses. Here we demonstrate the enrichment of lactic acid bacteria in a controlled sequencing batch bioreactor environment using a glucose‐based medium supplemented with peptides and B vitamins. A mineral medium enrichment operated in parallel was dominated by Ethanoligenens species and fermented glucose to acetate, butyrate and hydrogen. The complex medium enrichment was populated by Lactococcus, Lactobacillus and Megasphaera species and showed a product spectrum of acetate, ethanol, propionate, butyrate and valerate. An intermediate peak of lactate was observed, showing the simultaneous production and consumption of lactate, which is of concern for lactic acid production purposes. This study underlines that the competitive advantage for lactic acid‐producing bacteria primarily lies in their ability to attain a high biomass specific uptake rate of glucose, which was two times higher for the complex medium enrichment when compared to the mineral medium enrichment. The competitive advantage of lactic acid production in rich media can be explained using a resource allocation theory for microbial growth processes.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures

Rombouts

Kranendonk

Regueira

et al. 2020

Biotech & Bioengineering

Self Cite

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show abstract

“…This script is based on determining the last moment of base dosage in a cycle, which indicates the end of the fermentation, and thus the growth. This method is explained in detail in the supplementary information section published previously (Rombouts et al, 2019). The maximum biomass-specific substrate conversion rate (q s max ) is calculated using µ max and Y x,s using the Herbert-Pirt equation (Pirt, 1965) and neglecting maintenance.…”

Section: Estimation Of µ Max From On-line Data Collected From Biorementioning

confidence: 99%

Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures

et al.

Self Cite

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Lactic acid-producing bacteria are important in many fermentations, such as the production of biobased plastics. Insight in the competitive advantage of lactic acid bacteria over other fermentative bacteria in a mixed culture enables ecology-based process design and can aid the development of sustainable and energy-efficient bioprocesses. Here we demonstrate the enrichment of lactic acid bacteria in a controlled sequencing batch bioreactor environment using a glucose-based medium supplemented with peptides and B vitamins. A mineral medium enrichment operated in parallel was dominated by Ethanoligenens species and fermented glucose to acetate, butyrate and hydrogen. The complex medium enrichment was populated by Lactococcus, Lactobacillus and Megasphaera species and showed a product spectrum of acetate, ethanol, propionate, butyrate and valerate. An intermediate peak of lactate was observed, showing the simultaneous production and consumption of lactate, which is of concern for lactic acid production purposes. This study underlines that the competitive advantage for lactic acid-producing bacteria primarily lies in their ability to attain a high biomass specific uptake rate of glucose, which was two times higher for the complex medium enrichment when compared to the mineral medium enrichment. The competitive advantage of lactic acid production in rich media can be explained using a resource allocation theory for microbial growth processes.

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“…Populations of Rhodobacter have displayed a higher 644 maximum growth rate (1.8-2.2 d -1 in an enrichment and 2.3-3.8 d -1 with isolates) about 2.6 645 times faster than Rhodopseudomonas on VFA (Alloul et al, 2019). Batch regimes primarily 646 select on growth rate: organisms deploy their maximum growth rate across most of a batch 647 period during which substrate concentrations are mostly not limiting (Rombouts et al, 2019). 648…”

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confidence: 99%

Enriching and aggregating purple non-sulfur bacteria in an anaerobic sequencing-batch photobioreactor for nutrient capture from wastewater

Cerruti

Stevens

Ebrahimi

et al. 2020

Preprint

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25Purple non-sulfur bacteria (PNSB), a guild of anoxygenic photomixotrophic organisms, rise 26 interest to capture nutrients from wastewater in mixed-culture bioprocesses. One challenge 27 targets the aggregation of PNSB biomass through gravitational separation from the treated 28 water to facilitate its retention and accumulation, while avoiding the need for membranes. We 29 aimed to produce an enriched, concentrated, well-settling, nutrient-removing PNSB biomass 30 using sequencing batch regimes (SBR) in an anaerobic photobioreactor. The stirred tank was 31 fed with a synthetic influent mimicking loaded municipal wastewater (430-860 mg CODAc LInf -32 1 , COD:N:P ratio of 100:36:4-100:11:2 m/m/m), operated at 30°C and pH 7, and continuously 33 irradiated with infrared (IR) light (>700 nm) at 375 W m -2 . After inoculation with activated 34 sludge at 0.1 g VSS L -1 , PNSB were rapidly enriched in a first batch of 24 h: the genus 35Rhodobacter reached 54% of amplicon sequencing read counts. SBR operations at volume 36 exchange ratio of 50% with decreasing hydraulic retention times (48 to 16 h; 1 to 3 cycles d -1 ) 37 and increasing volumetric organic loading rates (0.2 to 1.3 kg COD m -3 d -1 ) stimulated the 38 aggregation (compact granules of 50-150 μm), settling (sedimentation G-flux of 4.7 kg h -1 m -39 2 ), and accumulation (as high as 3.8 g VSS L -1 ) of biomass. The sludge retention time (SRT) 40 increased freely from 2.5 to 11 d without controlled sludge wasting. Acetate, ammonium, and 41 orthophosphate were removed simultaneously (up to 96% at a rate of 1.1 kg COD m -3 d -1 , 77% 42 at 113 g N m -3 d -1 , and 73% at 15 g P m -3 d -1 ) with a COD:N:P assimilation ratio of 100:6.7:0.9 43 (m/m/m). Competition for substrate and photons occurred in the PNSB guild. SBR regime shifts 44 sequentially selected for Rhodobacter (90%) under shorter SRT and non-limiting acetate 45 concentrations during reaction phases, Rhodopseudomonas (70%) under longer SRT and 46 acetate limitation, and Blastochloris (10%) under higher biomass concentrations. We 47 Highlights 56• PNSB were highly enriched (90%) in an anaerobic stirred-tank photobioreactor. 57• The mixed-culture SBR process fostered PNSB biomass aggregation and accumulation. 58• PNSB sludge reached 3.8 g VSS L -1 and a sedimentation G-flux of 4.7 kg h -1 m -2 . 59

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Diversity and metabolism of xylose and glucose fermenting microbial communities in sequencing batch or continuous culturing

Abstract: Diversity and metabolism of xylose and glucose fermenting microbial communities in sequencing batch or continuous culturing. FEMS Microbiology Ecology, 95(2), [fiy233].

Cited by 34 publications

References 53 publications

Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures

Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures

Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures

Enriching and aggregating purple non-sulfur bacteria in an anaerobic sequencing-batch photobioreactor for nutrient capture from wastewater

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