Diversity and PAH growth abilities of bacterial strains isolated from a contaminated soil in Slovakia

Abstract

Different abandoned industrial areas contaminated by polycyclic aromatic hydrocarbons (PAHs) are present in Slovakia. These environmental burdens are very dangerous to the health of human and environment. The bioremediation, based on the use of hydrocarbons degrading microorganisms, is a promising strategy to sanitize these polluted sites. The aim of this investigation was to assess the bacterial diversity of a PAHs-contaminated soil and to select the potential hydrocarbonoclastic bacteria which can be used for different bioremediation approaches. The bacterial strains were isolated on minimal medium agar supplemented with a mixture of PAHs. Seventy-three isolated strains were grouped by ribosomal interspacer analysis in 15 different clusters and representatives of each cluster were identified by 16S rRNA sequencing. The PAHs degradation abilities of all bacterial isolates were estimated by the 2,6-dichlorophenol indophenol assay and by their growth on minimal broth amended with a mixture of PAHs. Different kinds of strains, members of the genus Pseudomonas, Enterobacter, Bacillus, Arthrobacter, Acinetobacter and Sphingomonas, were isolated from the contaminated soil. Four isolates (Pseudomonas putida, Arthrobacter oxydans, Sphingomonas sp. and S. paucimobilis) showed promising PAHs-degrading abilities and therefore their possible employing in bioremediation strategies.

Biosurfactant production by Pseudomonas aeruginosa DSVP20 isolated from petroleum hydrocarbon-contaminated soil and its physicochemical characterization

“…It is well documented that various bacteria from genus Pseudomonas, including P. aeruginosa strains, inhabit hydrocarbon-contaminated soils and can break a wide range of different organic compounds (Glick et al 1994;Hong et al 2005;Saikia et al 2012;Pacwa-Płociniczak et al 2014). They are the well-known bacteria that have the capability of utilizing a number of aliphatic and aromatic hydrocarbon compounds as a carbon and energy sources (Das and Chandran 2010;Kadali et al 2012;Puškárová et al 2013). Monitoring of maximum biosurfactant production and reduction in surface tension during growth of P. aeruginosa DSVP20 on pristane, eicosane, and fluoranthene at intervals of 24 h revealed that with increase in dry biomass, there was a reduction in This coincides with the maximum biosurfactant production (g/l) 6.315, 6.813, and 3.932 on pristane, eicosane, and fluoranthene, respectively ( Fig.…”

Bacterial endophytes from arid land plants regulate endogenous hormone content and promote growth in crop plants: an example of Sphingomonas sp. and Serratia marcescens

“…was found to contain genes responsible for carbazole degradation. Additionally, it can regulate certain classes of pesticides such as dibenzo-p-dioxins and remediate heavy metals (Puškárová et al 2013). It also possesses the potential to accumulate intracellular Zn 2+ , and reduction of Cd 2+ uptake enhanced the expression of the low-molecular weight cysteine-rich protein metallothionein, which sequesters heavy metals and their binding via associated proteins .…”

“…Sphingomonas sp. has recently been shown to help in the degradation of persistent metabolites in the environment (Puškárová et al 2013). Sphingomonas sp.…”

“…Sphingomonas belongs to a group of gramnegative bacteria producing yellow-pigmented colonies and is present in a diverse range of environments. The bacterium is metabolically versatile, as it can utilize a wide range of naturally occurring compounds as well as some types of environmental contaminants (Miyauchi et al 1998;Aylward et al 2013;Puškárová et al 2013). Sphingomonas sp.…”

Profiling microbial community structures across six large oilfields in China and the potential role of dominant microorganisms in bioremediation

“…Arthrobacter spp. have been reported to degrade various hydrocarbons, including aromatic hydrocarbons (Stevenson 1967) and PAHs (Puškárová et al 2013). Additionally, Arthrobacter spp.…”