Diversity and pathogenecity of <i>Vibrio</i> species in cultured bivalve molluscs

Beaz-Hidalgo, Roxana; Balboa, Sabela; Romalde, Jesús L.; Figueras, María José

doi:10.1111/j.1758-2229.2010.00135.x

Cited by 142 publications

(88 citation statements)

References 86 publications

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“…Furthermore, environmental characteristics, such as water temperature and salinity, are also known to influence the diversity of Vibrio spp. in the environment (Beaz-Hidalgo et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Population dynamics of Vibrio spp. associated with marine sponge microcosms

et al. 2010

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Vibrio is a diverse genus of marine-associated bacteria with at least 74 species and more expected as additional marine ecospheres are interrogated. This report describes a phylogenetic reconstruction of Vibrio isolates derived from one such unique ecosystem, marine sponges (Phylum Porifera) collected from depths of 150 to 1242 feet. 16S rRNA gene sequencing along with molecular typing of 16S-23S rRNA intergenic spacer regions clustered many sponge-associated Vibrio (spp) with current known species. That is, several benthic Vibrio species commensal with Porifera sponges seemed genetically linked to vibrios associated with coastal or shallow-water communities, signalling a panmictic population structure among seemingly ecologically disparate strains. Conversely, phylogenetic analysis provided evidence for at least two novel Vibrio speciation events within this specific sponge microcosm. Collectively, these findings earmark this still relatively unknown environment as a bastion of taxonomic and phylogenetic variability for the genus and probably other bacterial taxa.

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“…Furthermore, environmental characteristics, such as water temperature and salinity, are also known to influence the diversity of Vibrio spp. in the environment (Beaz-Hidalgo et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Population dynamics of Vibrio spp. associated with marine sponge microcosms

et al. 2010

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“…The question of why larvae become 'desperate' is interesting: the older oyster larvae which we observed were in good condition and well fed, so what triggers their desperation to settle? If larvae choose to remain in the water column, they are exposed to its dangers: larvae in late stages remain vulnerable to predators [168,169] and infection [170], as well as transport away from coastal settlement sites [171]. Further, patchiness of nutrients [172,173] makes a continued food supply uncertain.…”

Section: Discussionmentioning

confidence: 99%

Behavioral responses of invertebrate larvae to water column cues

Wheeler¹

2016

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Many benthic marine invertebrates have two-phase life histories, relying on planktonic larval stages for dispersal and exchange of individuals between adult populations. Historically, larvae were considered passive drifters in prevailing ocean currents. More recently, however, the paradigm has shifted toward active larval behavior mediating transport in the water column. Larvae in the plankton encounter a variety of physical, chemical, and biological cues, and their behavioral responses to these cues may directly impact transport, survival, settlement, and successful recruitment.In this thesis, I investigated the effects of turbulence, light, and conspecific adult exudates on larval swimming behavior. I focused on two invertebrate species of distinct morphologies: the purple urchin Arbacia punctulata, which was studied in pre-settlement planktonic stages, and the Eastern oyster Crassostrea virginica, which was studied in the competent-to-settle larval stage. From this work, I developed a conceptual framework within which larval behavior is understood as being driven simultaneously by external environmental cues and by larval age.As no a priori theory for larval behavior is derivable from first principles, it is only through experimental work that we are able to access behaviors and tie them back to specific environmental triggers. In this work, I studied the behavioral responses of larvae at the individual level, but those dynamics are likely playing out at larger scales in the ocean, impacting population connectivity, community structure, and resilience. In this way, my work represents progress in understanding how the ocean environment and larval behavior couple to influence marine ecological processes.

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“…Vibrio bacteria are a major cause of disease occurring in the marine environment (Demírcan and Candan, 2006) causing high mortality in some bivalves (Beaz-Hidalgo et al, 2010). Although the pathologies caused by Vibrio in bivalves have been described since the 1960s (Paillard et al, 2004) to this day, some of these species, such as V. alginolyticus, V. splendidus, V. anguillarum, are still being reported in case studies.…”

Section: E Martins Et Al: Immune Gene Expression Differences Inducementioning

confidence: 99%

Finding immune gene expression differences induced by marine bacterial pathogens in the Deep-sea hydrothermal vent mussel <i>Bathymodiolus azoricus</i>

et al. 2013

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Abstract. The deep-sea hydrothermal vent mussel Bathymodiolus azoricus lives in a natural environment characterised by extreme conditions of hydrostatic pressure, temperature, pH, high concentrations of heavy metals, methane and hydrogen sulphide. The deep-sea vent biological systems represent thus the opportunity to study and provide new insights into the basic physiological principles that govern the defense mechanisms in vent animals and to understand how they cope with microbial infections. Hence, the importance of understanding this animal's innate defense mechanisms, by examining its differential immune gene expressions toward different pathogenic agents. In the present study, B. azoricus mussels were infected with single suspensions of marine bacterial pathogens, consisting of Vibrio splendidus, Vibrio alginolyticus, or Vibrio anguillarum, and a pool of these Vibrio bacteria. Flavobacterium suspensions were also used as a non-pathogenic bacterium. Gene expression analyses were carried out using gill samples from infected animals by means of quantitative-Polymerase Chain Reaction aimed at targeting several immune genes. We also performed SDS-PAGE protein analyses from the same gill tissues.We concluded that there are different levels of immune gene expression between the 12 h to 24 h exposure times to various bacterial suspensions. Our results from qPCR demonstrated a general pattern of gene expression, decreasing from 12 h over 24 h post-infection. Among the bacteria tested, Flavobacterium is the bacterium inducing the highest gene expression level in 12 h post-infections animals. The 24 h infected animals revealed, however, greater gene expression levels, using V. splendidus as the infectious agent. The SDS-PAGE analysis also pointed at protein profile differences between 12 h and 24 h, particularly evident for proteins of 18-20 KDa molecular mass, where most dissimilarity was found. Multivariate analyses demonstrated that immune genes, as well as experimental infections, clustered in discrete groups in accordance with the gene expression patterns induced by bacterial pathogens.

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Diversity and pathogenecity of Vibrio species in cultured bivalve molluscs

Cited by 142 publications

References 86 publications

Population dynamics of Vibrio spp. associated with marine sponge microcosms

Population dynamics of Vibrio spp. associated with marine sponge microcosms

Behavioral responses of invertebrate larvae to water column cues

Finding immune gene expression differences induced by marine bacterial pathogens in the Deep-sea hydrothermal vent mussel <i>Bathymodiolus azoricus</i>

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Diversity and pathogenecity of Vibrio species in cultured bivalve molluscs

Cited by 142 publications

References 86 publications

Population dynamics of Vibrio spp. associated with marine sponge microcosms

Population dynamics of Vibrio spp. associated with marine sponge microcosms

Behavioral responses of invertebrate larvae to water column cues

Finding immune gene expression differences induced by marine bacterial pathogens in the Deep-sea hydrothermal vent mussel &lt;i&gt;Bathymodiolus azoricus&lt;/i&gt;

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Finding immune gene expression differences induced by marine bacterial pathogens in the Deep-sea hydrothermal vent mussel <i>Bathymodiolus azoricus</i>