Diversity and pathogenicity of Botryosphaeriaceae species associated with black root rot and stem cutting dry rot in Manihot esculenta in Brazil

Brito, A. C. Q.; Mello, J. F. De; Câmara, Marcos; Vieira, Janiele Cássia Barbosa; Michereff, Sami Jorge; Souza-Motta, Cristina Maria de; Machado, Alexandre Reis

doi:10.1007/s10658-020-02024-7

Cited by 26 publications

(27 citation statements)

References 40 publications

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“…For puri cation, the isolates were transferred to 3% Water Agar (WA) medium. After three days of growth a hyphal tip was collected and transferred onto PDA and maintained at 25°C for seven days in the dark (Brito et al 2020). After this period, those isolates were stored in tubes with sterile distilled water (Castellani 1967).…”

Section: Methodsmentioning

confidence: 99%

Richness of Nigrospora spp. (Apiosporaceae) in Manihot esculenta Cranz in Brazil and the description of three new species

Brito

Mello

Souza

et al. 2022

Preprint

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Manihot esculenta Crantz, commonly known as cassava, is an important staple food in developing countries in Africa, Asia and Latin America. Despite its relevance, few studies in search of endophytic fungi have been carried out on this plant. Nigrospora spp. has a widespread range of hosts and can be found as saprobes, endophytes, and pathogens. Nigrospora has already been isolated from cassava plants in previous studies. However, its identification was made only at the genus level. In this study, healthy cassava stems were collected in the northeast region of Brazil from where specimens of this genus were obtained. The isolates were identified based on morphological features and phylogenetic analyses of internal transcriber space (ITS), part of the translation elongation factor 1 alpha (TEF1- α), and β-tubulin fragment (TUB2) genic regions. As a result, we report two new geographic occurrences of N. pyriformis, and N. vesicularifera, four new records N. hainanensis, N. lacticolonia, N. pyriformis, and N. vesicularifera associated with cassava, and the description of three new species named N. endophytica, N. manihoticola and N. pernambucoensis.

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Section: Methodsmentioning

confidence: 99%

Richness of Nigrospora spp. (Apiosporaceae) in Manihot esculenta Cranz in Brazil and the description of three new species

Brito

Mello

Souza

et al. 2022

Preprint

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“…The distribution of this genus has extended to all continents. Neoscytalidium genus can influence the different parts of plants by causing diseases that show different symptoms on aerial and underground parts of hosts including dieback, elongated canker 8 , brown spot 9 , collar and root rot 10 , fruit internal brown rot 11 , stem and fruit canker 12 , leaf blight 13 , canker, shoot blight and fruit rot 14 , root rot 15 , black canker and root rot 16 , defoliation, root rot, inner stem necrosis, and plant death 2 , shoot and needle blight 17 , tuber rot 18 , black and dry root rot, and stem rot 19 , shoot blight 20 .…”

Section: Introductionmentioning

confidence: 99%

Neoscytalidium novaehollandiae causes dieback on Pinus eldarica and its potential for infection of urban forest trees

Alizadeh

Safaie

Shams‐Bakhsh

et al. 2022

Sci Rep

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Neoscytalidium novaehollandiae is one of the most important pathogens on woody plants which has increasingly been reported as a pathogen in different hosts in recent years. The pine trees are widely cultured in many cities of Iran. In recent years, dieback symptoms were observed on Pinus eldarica trees in Tehran and Qazvin provinces. The aim of this study was to investigate the dieback causal agent on P. eldarica trees in Iran. The branches and cones of P. eldarica trees were sampled for fungal isolation. The morphological and molecular characterizations (ITS, LSU, and TEF1-α regions) identified N. novaehollandiae as a dieback causal agent. This is the first report of N. novaehollandiae disease of P. eldarica trees in Iran. Furthermore, disease severity was assayed on 19 urban forest trees under three different temperature and relative humidity (RHs) regimes. C regime (29 °C and 15% RH) displayed more disease severity on detached branches than B (24 °C and 80% RH) and A (19 °C and 35% RH) ones. This study presents the host range of this pathogen, and showed that these potential hosts are prone to this pathogen under high temperature and low humidity which urban forest trees experienced in recent decades.

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“…Black rot is mainly caused by species from the Botryosphareacea family, such as Lasiodiplodia spp. and Neoscytalidium dimidiatum [ 11 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, other integrated disease management practices remain incipient, such as the use of biological or chemical controls and even the induction of soil suppressiveness through the addition of organic matter [ 22 ]. However, the presence of different plant pathogen genera in the same crop area leads to the need to develop cassava varieties that are multi-resistant to the root rot complex [ 10 , 16 , 18 , 19 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits

et al. 2022

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Cassava root rot disease is caused by a complex of soil-borne pathogens and has high economic impacts because it directly affects the tuberous roots, which are the main commercial product. This study aimed to evaluate cassava genotypes for resistance to root rot disease in a field with a previous history of high disease incidence. It also aimed to identify possible genomic regions associated with field resistance based on genome-wide association studies. A total of 148 genotypes from Embrapa Mandioca and Fruticultura were evaluated over two years, including improved materials and curated germplasms. Analysis of phenotypic data was conducted, as well as a genomic association analysis, based on the general linear model, mixed linear model, and fixed and random model circulating probability unification. The observed high disease index (ω) was directly correlated with genotype survival, affecting plant height, shoot yield, and fresh root yield. The genotypes were grouped into five clusters, which were classified according to level of root rot resistance (i.e., extremely susceptible, susceptible, moderately susceptible, moderately resistant, and resistant). The 10 genotypes with the best performance in the field were selected as potential progenitors for the development of segregating progenies. Estimates of genomic kinship between these genotypes ranged from -0.183 to 0.671. The genotypes BGM-1171 and BGM-1190 showed the lowest degree of kinship with the other selected sources of resistance. The genotypes BGM-0209, BGM-0398, and BGM-0659 showed negative kinship values with most elite varieties, while BGM-0659 presented negative kinship with all landraces. A genome-wide association analysis detected five significant single nucleotide polymorphisms related to defense mechanisms against biotic and abiotic stresses, with putative association with fresh root yield in soil infested with root rot pathogens. These findings can be utilized to develop molecular selection for root rot resistance in cassava.

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Diversity and pathogenicity of Botryosphaeriaceae species associated with black root rot and stem cutting dry rot in Manihot esculenta in Brazil

Cited by 26 publications

References 40 publications

Richness of Nigrospora spp. (Apiosporaceae) in Manihot esculenta Cranz in Brazil and the description of three new species

Richness of Nigrospora spp. (Apiosporaceae) in Manihot esculenta Cranz in Brazil and the description of three new species

Neoscytalidium novaehollandiae causes dieback on Pinus eldarica and its potential for infection of urban forest trees

Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits

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