Diversity and phylogeny of plant growth‐promoting bacilli from moderately acidic soil

Abstract

The molecular diversity of aerobic endospore-forming bacteria, typically Bacillus and its derived genera, has been investigated in various environments. However, there have been few investigations concerning Bacillus in acidic soils. In this study, the genotypic diversity and phylogenetic relationships among plant growth-promoting (PGP) bacilli isolated from the rice rhizosphere growing in acidic soils of Kerala (pH varying from 6.3 to 6.8) were investigated. For assessing their biocontrol potential and PGP attributes, 115 isolates were randomly selected and 49 isolates that were positive for multiple traits were selected. Metabolic characterization of representative strains, using the Biolog GP2 (Gram Positive) MicroPlate(TM) , revealed a large versatility with respect to carbohydrate utilization. Amplified ribosomal DNA restriction analysis revealed 13 clusters at 65% similarity level, which consisted of 1-21 strains. 16S rDNA partial sequencing assigned all the isolates, except for one, to the Bacillus genus, with close relatedness to Bacillus humi, B. megaterium, B. drentensis, B. pocheonensis, B. aestuarii, B. arbutinivorans, B. niacini, and Brevibacterium casei. The Bacillus species with different metabolic capabilities, PGP abilities, and genetic diversity found in this study are likely to have ecological relevance.

Evaluating the Diversity of Culturable Thermotolerant Bacteria from Four Hot Springs of India

“…B3 was able to utilize only 35.71% of the substrates tested. Strains of Bacillus pumilus generally show high resistance to environmental stresses, including UV light exposure, desiccation, and the presence of oxidizers such as hydrogen peroxide and produce compounds antagonist to fungal and bacterial pathogens [17]. In the present investigation, this strain was able to show growth on 45% of the substrates and chemical agents tested, besides being uniquely able to use D-Arabitol, and uniquely exhibiting resistance to guanidine and sodium butyrate (Table 3).…”

“…At 90% similarity level, thermotolerant isolates from Bakreshwar, Balrampur, Chumathang and Vashist thermal springs grouped into 14, 14, 11 and 9 clusters respectively. ARDRA has been effectively utilized in the past for both culture dependent and culture independent diversity studies as a tool to group the isolates and clones into phylotypes [17][18][19].…”

“…Amplified ribosomal DNA restriction analysis (ARDRA) represents a further improvement in the analysis of 16S rRNA genes and involves amplification of 16S rRNA gene followed by digestion of amplified product using selected restriction enzymes and generation of restriction patterns. Restriction patterns can then be compared [17] and analysed using bioinformatic tools. The utility of ARDRA in genotypic characterization of bacteria [18] and screening clone libraries [19] for grouping them into phylogenetic clusters is undisputed.…”

Variations in the structure of airborne bacterial communities in a downwind area during an Asian dust (Kosa) event

“…In contrast, many Bacillus strains have been exploited for biotechnological applications (Hou et al, 2005) and have been shown to contribute to the promotion of plant growth (Yadav et al, 2011). Thus, the transport of airborne bacteria provides a mechanism for the dispersal of bacterial populations that can have both beneficial and negative influences on human health and plant growth.…”

Identification of Water Bacteria from Nickel Post Mining in East Halmahera

“…diduga menjadi dominan pada kondisi lingkungan yang ekstrim. Yadav et al (2011) menyebutkan bahwa genus Bacillus mempunyai rentang keragaman spesies yang dominan pada berbagai kondisi lingkungan termasuk pada kondisi lingkungan yang ekstrim. Tidak hanya pada kondisi tanah yang ekstrim, Bacillus juga menjadi jenis yang dominan ditemukan pada makanan yang dibuat dengan proses fermentasi dengan kondisi asam yang tinggi (Kim et al, 2014).…”