Diversity and Role of Bacterial Integron/Gene Cassette Metagenome in Extreme Marine Environments

Elsaied, Hosam; Maruyama, Akihiko

doi:10.1002/9781118010549.ch31

Cited by 4 publications

(2 citation statements)

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“…The most obvious examples of these proteins are transferase enzymes and ion transporter proteins, which have been recorded in several marine environments (Elsaied et al ., , ; Wright et al ., ). Some specific cassette proteins, such as those related to sulfur metabolism, have been recorded and confirmed by gene expression, in a sulfur‐oxidizing deep‐sea endosymbiont (Elsaied et al ., ; Elsaied & Maruyama, ). A link between adaptive potential and integron cassettes has also been recently inferred in a Vibrio species, where it was found that deletion of cassettes can impact on the growth potential of the strain Vibrio rotiferianus DAT722 in a way that is linked to changes in cell surface properties (Rapa et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Novel integrons and gene cassettes from a Cascadian submarine gas-hydrate-bearing core

Elsaied

Stokes

Yoshioka

et al. 2013

FEMS Microbiol Ecol

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To determine whether integrons are present in a submarine gas hydrate community, metagenomic DNA was extracted from a gas-hydrate-bearing core, 150 m below the seafloor, from the Cascadian Margin. Integrons and gene cassettes were recovered by PCR from metagenomic DNA and sequenced. Thirty-seven integron integrase phylotypes were identified. The phylotypes were diverse and included members with homology to integrases from Methylomonas methanica, Desulfuromonas acetoxidans, Thermodesulfatator indicus, and marine uncultured bacteria. The gene cassette composition, 153 gene cassettes, was dominated by two types of encoded putative proteins. The first of these was predicted oxidoreductases, such as iron/sulfur cluster-binding proteins. A second type was alkyl transferases. Some cassette proteins showed homologies with those from methane-related archaea. These observations suggest that integrons may assist in the adaptation of microbial communities in this environment.

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Section: Introductionmentioning

confidence: 99%

Novel integrons and gene cassettes from a Cascadian submarine gas-hydrate-bearing core

Elsaied

Stokes

Yoshioka

et al. 2013

FEMS Microbiol Ecol

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“…At least three integron classes contribute to the spread of antibiotic resistance ( Partridge et al , 2009 ). Outside the antibiotic-resistance domain, the biological significance of environmental integrons was demonstrated in their flexibility to acquire and express adaptive genes for several environmental stresses ( Elsaied et al , 2007 ; Robinson et al , 2007 ; Koenig et al , 2009 ; Elsaied and Maruyama, 2010 ). This unique integron feature creates a system that provides for an enormous pool of adaptive genes to be mobilized, rearranged and disseminated among environmental bacteria.…”

Section: Introductionmentioning

confidence: 99%

Marine integrons containing novel integrase genes, attachment sites, attI, and associated gene cassettes in polluted sediments from Suez and Tokyo Bays

et al. 2011

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In order to understand the structure and biological significance of integrons and associated gene cassettes in marine polluted sediments, metagenomic DNAs were extracted from sites at Suez and Tokyo Bays. PCR amplicons containing new integrase genes, intI, linked with novel gene cassettes, were recovered and had sizes from 1.8 to 2.5 kb. This approach uncovered, for the first time, the structure and diversity of both marine integron attachment site, attI, and the first gene cassette, the most efficiently expressed integron-associated gene cassette. The recovered 13 and 20 intI phylotypes, from Suez and Tokyo Bay samples, respectively, showed a highly divergence, suggesting a difference in integron composition between the sampling sites. Some intI phylotypes showed similarity with that from Geobacter metallireducens, belonging to Deltaproteobacteria, the dominant class in both sampling sites, as determined by 16S rRNA gene analysis. Thirty distinct families of putative attI site, as determined by the presence of an attI-like simple site, were recovered. A total of 146 and 68 gene cassettes represented Suez and Tokyo Bay unsaturated cassette pools, respectively. Gene cassettes, including a first cassette, from both sampling sites encoded two novel families of glyoxalase/bleomycin antibiotic-resistance protein. Gene cassettes from Suez Bay encoded proteins similar to haloacid dehalogenases, protein disulfide isomerases and death-on-curing and plasmid maintenance system killer proteins. First gene cassettes from Tokyo Bay encoded a xenobiotic-degrading protein, cardiolipin synthetase, esterase and WD40-like β propeller protein. Many of the first gene cassettes encoded proteins with no ascribable function but some of them were duplicated and possessed signal functional sites, suggesting efficient adaptive functions to their bacterial sources. Thus, each sampling site had a specific profile of integrons and cassette types consistent with the hypothesis that the environment shapes the genome.

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Marine Microbial Community Adaptation and Resiliency to Anthropogenic Stresses Through Horizontal Gene Transfer

Rajan

Sobecky

2017

Microbial Ecotoxicology

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Diversity and Role of Bacterial Integron/Gene Cassette Metagenome in Extreme Marine Environments

Cited by 4 publications

References 35 publications

Novel integrons and gene cassettes from a Cascadian submarine gas-hydrate-bearing core

Novel integrons and gene cassettes from a Cascadian submarine gas-hydrate-bearing core

Marine integrons containing novel integrase genes, attachment sites, attI, and associated gene cassettes in polluted sediments from Suez and Tokyo Bays

Marine Microbial Community Adaptation and Resiliency to Anthropogenic Stresses Through Horizontal Gene Transfer

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