Diversity and Spatiotemporal Distribution of Fungal Endophytes Associated with Citrus reticulata cv. Siyahoo

Sadeghi, Fatemeh; Samsampour, Davood; Seyahooei, Majeed Askari; Bagheri, Abdoolnabi; Soltani, Jalal

doi:10.1007/s00284-019-01632-9

Cited by 52 publications

(26 citation statements)

References 49 publications

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“…The microbial endophyte community in leaves typically differed from those in stems for the two cultivars assessed (‘Royal Gala’ and ‘Braeburn’). This was consistent with previous studies (da Silva et al ; Win et al ; Sadeghi et al ). It is likely due to both ontogenic differences and the mechanisms that recruit micro‐organisms to these tissues in apples (Chi et al ; Herre et al ; Bulgarelli et al ).…”

Section: Discussionsupporting

confidence: 94%

“…This may result in a different response of plant‐associated microbiota to region variability. Season influenced the abundance of actinobacteria and proteobacteria in branches of different tree species Acer negundo (Manitoba Maple) , Ulmus pumila (Siberian Elm) and U. parvifolia (Chinese Elm) (Shen and Fulthorpe ) and bacterial and fungal endophytic colonization and/or isolation rate in other plant species related to temperature and wetness period and growth stage of the tissue (Mocali et al ; Guo et al ; Rather et al ; Sadeghi et al ). In contrast to these other studies, the smaller amount of variation according to region and season is likely to have been partially due to solely woody stems, the infection court of the pathogen, being assessed.…”

Section: Discussionmentioning

confidence: 99%

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Apple endophyte community is shaped by tissue type, cultivar and site and has members with biocontrol potential againstNeonectria ditissima

2020

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Aims This research aimed to identify factors influencing endophyte community structure in apple shoots and the bioactivity of cultured representatives against the fungal pathogen Neonectria ditissima. Methods and Results The endophyte community in leaves and stems of the apple cultivars ‘Royal Gala’ and ‘Braeburn’ were analysed by a cultivation‐independent method (PCR‐DGGE) which showed that tissue type, cultivar and site were determinant factors, with the endophyte taxa in ‘Royal Gala’ more variable than that in ‘Braeburn’, with leaf endophyte communities typically differing from stems in both cultivars. Seasonal (spring vs autumn) and regional (Nelson vs Hawke’s Bay) variations were not obvious in woody stems. A collection of 783 bacterial and 87 fungal endophytes were recovered from leaves and stems of ‘Royal Gala’, ‘Braeburn’, ‘Scilate’ and/or ‘Scifresh’ from Nelson (nine sites) and Hawke’s Bay (five sites) in spring and from Nelson (three sites) in autumn. A dual culture plating assay was used to test their ability to inhibit the mycelial growth of N. ditissima. Thirteen bacterial (mean of percent inhibition ≥20%) and 17 fungal isolates were antagonistic towards N. ditissima. These isolates belonged to the bacterial genera Bacillus and Pseudomonas, and fungal genera Chaetomium, Epicoccum, Biscogniauxia, Penicillium, Diaporthe, Phlyctema and two unidentified fungal isolates. Conclusions Endophyte communities in apple shoots were determined by tissue type, cultivar and site. Endophytic bacterial and fungal isolates inhibiting N. ditissima growth in vitro were found. Significance and Impact of the Study These results provided new evidence of factors influencing apple endophyte community in New Zealand. Endophytes with potential to reduce N. ditissima infection were identified, with the potential to be developed into a biocontrol strategy for European canker.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Apple endophyte community is shaped by tissue type, cultivar and site and has members with biocontrol potential againstNeonectria ditissima

2020

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“…Besides, the diversity of fungal endophyte communities in leaf, stem, trunk, and root tissues of C. reticulata cv. Siyahoo was observed 38 . However, a high similarity of dominant bacterial communities of Pericarpium Citri Reticulatae 'Chachiensis' obtained from different orchards in Xinhui District was observed 39 .…”

Section: Discussionmentioning

confidence: 99%

Distinct microbial communities among different tissues of citrus tree Citrus reticulata cv. Chachiensis

et al. 2020

Sci Rep

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Plant microbiota colonize all organs of a plant and play crucial roles including supplying nutrients to plants, stimulating seed germination, promoting plant growth, and defending plants against biotic and abiotic stress. Because of the economic importance, interactions between citrus and microbes have been studied relatively extensively, especially citrus-pathogen interactions. However, the spatial distribution of microbial taxa in citrus trees remains under-studied. In this study, Citrus reticulata cv. Chachiensis was examined for the spatial distribution of microbes by sequencing 16S rRNA genes. More than 2.5 million sequences were obtained from 60 samples collected from soil, roots, leaves, and phloem. The dominant microbial phyla from all samples were Proteobacteria, Actinobacteria and Acidobacteria. The composition and structure of microbial communities in different samples were analyzed by PCoA, CAP, Anosim and MRPP methods. Variation in microbial species between samples were analyzed and the indicator microbes of each sample group were identified. Our results suggested that the microbial communities from different tissues varied significantly and the microenvironments of tree tissues could affect the composition of its microbial community.www.nature.com/scientificreports www.nature.com/scientificreports/ trees 23 . Most previous investigation on citrus-associated microbiomes are analyses on huanglongbing-infected, or specially-treated samples [24][25][26][27][28][29] .Citrus reticulata cv. Chachiensis is an important citrus variety widely cultivated around the world and the orange peel of the fruit was regarded as the valuable material which could be processed as food, tea drinks, and seasoning 30 . Besides, the orange peel owned antioxidant properties and extremely high medicinal value was also used as herb-medicine 31 . In China, C. reticulata cv. Chachiensis has grown in Xinhui District, Jiangmen City, Guangdong Province was considered as one of the most important cash crops for locals and the orange peel named genuine Pericarpium Citri Reticulatae (Guang Chen Pi) was admired as the best Chen pi which possesses the excellent clinical efficacy 32 . Plant-related microorganisms could protect the host from pathogens and pests, help plants use nutrients, and improve plant stress resistance 33 , while the microbial community of C. reticulata cv. Chachiensis remains unknown. In this study, endosphere microbiomes in soils, roots, leaves, and phloem of C. reticulata cv. Chachiensis were systematically investigated by sequencing 16S rRNA genes. The composition and structure of the microbial community in different tissues were performed by PCoA, CAP, Anosim and MRPP methods. Variation in microbial compositions among citrus tissues were analyzed and indicator microbes for different samples were identified. Our results suggested that the microbial community from different tissues of C. reticulata cv. Chachiensis in south china varied significantly. Our results laid a foundation for future studies on microbial comm...

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“…Encouraging examples in this direction are represented by two very recent publications from Iran [48,49]. Indeed, the focus on endophytic fungi is gradually evolving from a basically descriptive phase to the analysis of factors influencing the structure and composition of microbiomes, in view of their manipulation for increasing plant protection and productivity.…”

Section: Discussionmentioning

confidence: 99%

“…Even more species have been reported for their endophytic occurrence as a result of a phylogenetic reassessment carried out in China, with eight known (D. arecae species complex, D. citri, D. citriasiana, D. citrichinensis, D. endophytica, D. eres, D. hongkongensis, and D. sojae) and seven new species (D. biconispora, D. biguttulata, D. discoidispora, D. multiguttulata, D. ovalispora, D. subclavata, and D. unshiuensis) [45]. C. limon Cameroon [36] Endophytic occurrence has also been reported for other citrus pathogens, such as the leaf-spot agents Alternaria alternata [35,[46][47][48] and Alternaria citri [49], Fusarium oxysporum [48], and Fusarium sarcochroum, known as a possible agent of dieback of twigs on mandarin and lemon [50]. The latter study also introduces new Fusarium spp.…”

Section: Endophytic Occurrence Of Citrus Pathogensmentioning

confidence: 95%

Endophytic Fungi of Citrus Plants

Nicoletti

2019

Agriculture

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Besides a diffuse research activity on drug discovery and biodiversity carried out in natural contexts, more recently, investigations concerning endophytic fungi have started considering their occurrence in crops based on the major role that these microorganisms have been recognized to play in plant protection and growth promotion. Fruit growing is particularly involved in this new wave, by reason that the pluriannual crop cycle likely implies a higher impact of these symbiotic interactions. Aspects concerning occurrence and effects of endophytic fungi associated with citrus species are revised in the present paper.Agriculture 2019, 9, 247 2 of 13 similar but fast-growing, and producing conidia embedded within a thicker mucoid sheath [4][5][6][7][8]. The latter, characterized as a different species (Phyllosticta capitalensis), are known to be ubiquitous as endophytes in woody plants, having been reported from at least 70 botanical families [6,9,10]. Guignardia endophyllicola, treated as a separate species in a work also emphasizing its widespread endophytic occurrence [11], is at present recognized as a synonym. Differences between the two sister species also concern their metagenetic cycle. In fact, it has been ascertained that P. citricarpa is heterothallic, while P. capitalensis is homothallic [8]. This consolidated taxonomic distinction supports the exclusion from quarantine measures of plant material harbouring P. capitalensis. To this purpose, several rapid PCR assays have been developed [12][13][14][15][16][17][18][19][20]. The applicative use of these assays has enabled to exclude the presence of the pathogen in New Zealand, unlike what was previously assumed [21], and has supported the hypothesis of the possible endophytic occurrence of P. citricarpa in asymptomatic Citrus spp., as pointed out by several investigations (Table 1). Moreover, the two species have been clearly differentiated on account of their enzymatic profiles, with a higher expression of amylases, endoglucanases, and pectinases in P. citricarpa, suggesting a likely involvement of these enzymes in the pathogenic aptitude of the CBS agent [22]. Differences in terms of pathogenesis-related proteins have been confirmed after the genome sequencing of the two species, disclosing a higher number of coding sequences in P. citricarpa (15,206 versus 14,797). Such a difference has been interpreted considering the presence of growth and developmental genes involved in the expression of pathogenicity [23].The issue of detection of contaminated material imported from areas where the pathogen is endemic has also prompted investigations concerning the assortment of Phyllosticta spp. able to colonize citrus plants in either symptomatic or latent courses. Several revisions have been published [17,24], and novel species characterized, which consistently enlarge the citrus-associated consortium within this widespread genus. Particularly, the pathogenic P. citriasiana from south-east Asia [25], P. citrichinaensis from China [26], P. citrimaxima from Thailand...

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Diversity and Spatiotemporal Distribution of Fungal Endophytes Associated with Citrus reticulata cv. Siyahoo

Cited by 52 publications

References 49 publications

Apple endophyte community is shaped by tissue type, cultivar and site and has members with biocontrol potential againstNeonectria ditissima

Apple endophyte community is shaped by tissue type, cultivar and site and has members with biocontrol potential againstNeonectria ditissima

Distinct microbial communities among different tissues of citrus tree Citrus reticulata cv. Chachiensis

Endophytic Fungi of Citrus Plants

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