To estimate an activity of hydrolases of test-cultures of microscopic fungi that is applied for tests from funginertness of technical wares and materials. Methods. Enzymatic activities (of complexes of cellulases, amylases, lipases and proteases) determined in accordance with the methods, based on reactions between dye, that bring into medium or on a colony and the product of curriculum of substrate and expected enzymatic index. Results. It has been edused that all investigational isolates were characterized by the presence at least of one enzymatic activity (cellulase, amylase, lipase or protease) Conclusions. It has been established that enzymatic activities of isolates, except for C. globosum of F-16714, did not depend on a their expiration and source of selection date.. It has been educed that