Diversity and structure of bacterial communities in Fildes Peninsula, King George Island

Fan, Jing-Li; Li, Lili; Han, Junli; Ming, Hongxia; Li, Jiangyu; Na, Guangshui; Chen, Jiaying

doi:10.1007/s00300-013-1358-9

Cited by 11 publications

(8 citation statements)

References 28 publications

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Section: Different Magnitudes Of Microbiota Variability Among Sample supporting

confidence: 88%

“…The Fildes Peninsula in Antarctica remains today a remote and logistically challenging location with few description of its bacterial microbiota, especially of the shallow marine sediments (Foong et al, 2010;Fan et al, 2013). Here, Gammaproteobacteria and Bacteroidetes were dominant in the external sediment samples of both sites, thus confirming the previous findings in the literature reporting these classes as the most abundant in marine sediments of the Fildes Peninsula in Antarctica (Foong et al, 2010;Fan et al, 2013). It is also worth emphasizing that, despite the important differences in the protocol used (e.g., genomic DNA extraction, PCR primer, among others), we retrieved various OTUs (including the community-structuring ones) strictly identical to those previously identified in the marine benthic sediment of the King George Island (Vázquez et al, 2017), thus validating the robustness of our methodology.…”

Section: Different Magnitudes Of Microbiota Variability Among Sample mentioning

confidence: 99%

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Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii

et al. 2020

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Abatus agassizii is an irregular sea urchin species that inhabits shallow waters of South Georgia and South Shetlands Islands. As a deposit-feeder, A. agassizii nutrition relies on the ingestion of the surrounding sediment in which it lives barely burrowed. Despite the low complexity of its feeding habit, it harbors a long and twice-looped digestive tract suggesting that it may host a complex bacterial community. Here, we characterized the gut microbiota of specimens from two A. agassizii populations at the south of the King George Island in the West Antarctic Peninsula. Using a metabarcoding approach targeting the 16S rRNA gene, we characterized the Abatus microbiota composition and putative functional capacity, evaluating its differentiation among the gut content and the gut tissue in comparison with the external sediment. Additionally, we aimed to define a core gut microbiota between A. agassizii populations to identify potential keystone bacterial taxa. Our results show that the diversity and the composition of the microbiota, at both genetic and predicted functional levels, were mostly driven by the sample type, and to a lesser extent by the population location. Specific bacterial taxa, belonging mostly to Planctomycetacia and Spirochaetia, were differently enriched in the gut content and the gut tissue, respectively. Predictive functional profiles revealed higher abundance of specific pathways, as the sulfur cycle in the gut content and the amino acid metabolism, in the gut tissue. Further, the definition of a core microbiota allowed to obtain evidence of specific localization of bacterial taxa and the identification of potential keystone taxa assigned to the Desulfobacula and Spirochaeta genera as potentially host selected. The ecological relevance of these keystone taxa in the host metabolism is discussed.

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Section: Different Magnitudes Of Microbiota Variability Among Sample supporting

confidence: 88%

Section: Different Magnitudes Of Microbiota Variability Among Sample mentioning

confidence: 99%

Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii

et al. 2020

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“…Hitherto, diverse soil bacterial communities have been observed in Antarctic terrestrial ecosystems using traditional isolation methods (Powell et al, 2006 ; Rinnan et al, 2009 ; Fan et al, 2013 ; Zdanowski et al, 2013 ) and traditional molecular methods (e.g., PCR-DGGE, cloning sequencing, real-time PCR, microarray) (Smith et al, 2006 , 2010 ; Shravage et al, 2007 ; Yergeau et al, 2007 , 2009 ; Niederberger et al, 2008 ; Yergeau and Kowalchuk, 2008 ; Aislabie et al, 2009 ; Chong et al, 2009 , 2010 ; Soo et al, 2009 ; Newsham et al, 2010 ; Ganzert et al, 2011 ; Ma et al, 2012 ; Stomeo et al, 2012 ; Pan et al, 2013 ; Teixeira et al, 2013 ). Recently, greater phylogenetic diversity of soil bacterial communities has been found in the Antarctic soils using Roche 454 sequencing (Teixeira et al, 2010 ; Kim et al, 2012 ; Lee et al, 2012 ; Roesch et al, 2012 ; Tiao et al, 2012 ; Yergeau et al, 2012 ), which is able to identify a great number of bacterial sequences and provide an in-depth analysis of soil bacterial diversity.…”

Section: Introductionmentioning

confidence: 99%

Diversity and structure of soil bacterial communities in the Fildes Region (maritime Antarctica) as revealed by 454 pyrosequencing

Wang

Zhang

et al. 2015

Front. Microbiol.

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This study assessed the diversity and composition of bacterial communities in four different soils (human-, penguin-, seal-colony impacted soils and pristine soil) in the Fildes Region (King George Island, Antarctica) using 454 pyrosequencing with bacterial-specific primers targeting the 16S rRNA gene. Proteobacteria, Actinobacteria, Acidobacteria, and Verrucomicrobia were abundant phyla in almost all the soil samples. The four types of soils were significantly different in geochemical properties and bacterial community structure. Thermotogae, Cyanobacteria, Fibrobacteres, Deinococcus-Thermus, and Chlorobi obviously varied in their abundance among the 4 soil types. Considering all the samples together, members of the genera Gaiella, Chloracidobacterium, Nitrospira, Polaromonas, Gemmatimonas, Sphingomonas, and Chthoniobacter were found to predominate, whereas members of the genera Chamaesiphon, Herbaspirillum, Hirschia, Nevskia, Nitrosococcus, Rhodococcus, Rhodomicrobium, and Xanthomonas varied obviously in their abundance among the four soil types. Distance-based redundancy analysis revealed that pH (p < 0.01), phosphate phosphorus (p < 0.01), organic carbon (p < 0.05), and organic nitrogen (p < 0.05) were the most significant factors that correlated with the community distribution of soil bacteria. To our knowledge, this is the first study to explore the soil bacterial communities in human-, penguin-, and seal- colony impacted soils from ice-free areas in maritime Antarctica using high-throughput pyrosequencing.

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“…The positive correlations to each other within this module were possibly contributed to their common requirement of anaerobic niches. Besides obligate psychrophiles, such as Psychroserpens, Psychroflexus, Psychrobacter , some members of Marinobacter, Halomonas, Gillisia, Pseudomonas , and Flavobacteriaceae were also reported to show adaptive characteristics to low temperature, such as production of exopolysaccharides, cold active enzymes and pigments, and changes in membrane lipid composition and fluidity (Fan et al, 2013; Kumbhare et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, Antarctica

Han

Zhang

et al. 2019

Front. Microbiol.

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The benthic bacterial community in Antarctic continental shelf ecosystems are not well-documented. We collected 13 surface sediments from the Ross Sea, a biological hotspot in high-latitude maritime Antarctica undergoing rapid climate change and possible microflora shift, and aimed to study the diversity, structure and assembly mechanism of benthic bacterial community using both culture-dependent and -independent approaches. High-throughput sequencing of 16S rRNA gene amplicons revealed 370 OTUs distributed in 21 phyla and 284 genera. The bacterial community was dominated by Bacteroidetes, Gamma- and Alphaproteobacteria, and constituted by a compact, conserved and positively-correlated group of anaerobes and other competitive aerobic chemoheterotrophs. Null-model test based on βNTI and RCBray indicated that stochastic processes, including dispersal limitation and undominated fractions, were the main forces driving community assembly. On the other hand, environmental factors, mainly temperature, organic matter and chlorophyll, were significantly correlated with bacterial richness, diversity and community structure. Moreover, metabolic and physiological features of the prokaryotic taxa were mapped to evaluate the adaptive mechanisms and functional composition of the benthic bacterial community. Our study is helpful to understand the structural and functional aspects, as well as the ecological and biogeochemical role of the benthic bacterial community in the Ross Sea.

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Diversity and structure of bacterial communities in Fildes Peninsula, King George Island

Cited by 11 publications

References 28 publications

Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii

Characterization of the Gut Microbiota of the Antarctic Heart Urchin (Spatangoida) Abatus agassizii

Diversity and structure of soil bacterial communities in the Fildes Region (maritime Antarctica) as revealed by 454 pyrosequencing

Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, Antarctica

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