Diversity, heavy metals, and antibiotic resistance in culturable heterotrophic bacteria isolated from former lead–silver–zinc mine heap in Tarnowskie Gory (Silesia, Poland)

Nosalova, Lea; Willner, J.; Fornalczyk, A.; Saternus, M.; Sedláková-Kaduková, Jana; Piknová, Mária; Pristaš, Peter

doi:10.1007/s00203-022-03369-9

Cited by 6 publications

(4 citation statements)

References 55 publications

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“…However, in its genome, genes for the alpha ( ureC ), beta ( ureB ) and gamma ( ureA ) subunits of the urease enzyme complex are encoded, in addition to other subsets of accessory genes such as ureEFD and ureFDG ( Hoke et al, 2021 ). On the other hand, in Paenibacillus mucilaginosus , calcium and calcite biomineralization studies were conducted in industrial soils ( Zhan et al, 2016 ) and the presence of Paenibacillus has been described in soils contaminated with arsenic, antimony tailings, lignite mine wastes, abandoned lead-silver-zinc mining waste and around mine tailings with the presence of PTEs, thus demonstrating the ability of this group of microorganisms to tolerate these contaminants ( Shagol et al, 2014 ; Xiao et al, 2019 ; Karuriya and Choudhary, 2022 ; Nosalova et al, 2022 ; Wang et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, this genus of bacteria is widely distributed in soils contaminated with different PTEs such as Cu, Pb, Cr, Zn, As and Hg. These bacteria are supposed to play a role in soil remediation as they enhance plant settlement in contaminated areas ( Domingues et al, 2020 ; Fashola et al, 2020 ; Narayanan et al, 2020 ; Nosalova et al, 2022 ; El-Imam et al, 2023 ; Rojas-Solis et al, 2023 ; Xia et al, 2023 ). Like the other selected bacteria, it presents in its genome all the genes for urease synthesis ( ureA , ureB , ureC , ureE , ureF and ureD ), but unlike P. megaterium the urease activity in this bacterium is constitutive and when induced, its urease activity increases by 50% ( Oki et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

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MICP mediated by indigenous bacteria isolated from tailings for biocementation for reduction of wind erosion

Maureira,

Zapata,

Olave

et al. 2024

Front. Bioeng. Biotechnol.

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In this study, native ureolytic bacteria were isolated from copper tailings soils to perform microbial-induced carbonate precipitation (MICP) tests and evaluate their potential for biocement formation and their contribution to reduce the dispersion of particulate matter into the environment from tailings containing potentially toxic elements. It was possible to isolate a total of 46 bacteria; among them only three showed ureolytic activity: Priestia megaterium T130-1, Paenibacillus sp. T130-13 and Staphylococcus sp. T130-14. Biocement cores were made by mixing tailings with the isolated bacteria in presence of urea, resulting similar to those obtained with Sporosarcina pasteurii and Bacillus subtilis used as positive control. Indeed, XRD analysis conducted on biocement showed the presence of microcline (B. subtilis 17%; P. megaterium 11. 9%), clinochlore (S. pasteurii, 6.9%) and magnesiumhornblende (Paenibacillus sp. 17.8%; P. megaterium 14.6%); all these compounds were not initially present in the tailings soils. Moreover the presence of calcite (control 0.828%; Paenibacillus sp. 5.4%) and hematite (control 0.989%; B. subtilis 6.4%) was also significant unlike the untreated control. The development of biofilms containing abundant amount of Ca, C, and O on microscopic soil particles was evidenced by means of FE-SEM-EDX and XRD. Wind tunnel tests were carried out to investigate the resistance of biocement samples, accounted for a mass loss five holds lower than the control, i.e., the rate of wind erosion in the control corresponded to 82 g/m2h while for the biocement treated with Paenibacillus sp. it corresponded to only 16.371 g/m2h. Finally, in compression tests, the biocement samples prepared with P. megaterium (28.578 psi) and Paenibacillus sp. (28.404 psi) showed values similar to those obtained with S. pasteurii (27.102 psi), but significantly higher if compared to the control (15.427 psi), thus improving the compression resistance capacity of the samples by 85.2% and 84.1% with respect to the control. According to the results obtained, the biocement samples generated with the native strains showed improvements in the mechanical properties of the soil supporting them as potential candidates in applications for the stabilization of mining liabilities in open environments using bioaugmentation strategies with native strains isolated from the same mine tailing.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MICP mediated by indigenous bacteria isolated from tailings for biocementation for reduction of wind erosion

Maureira,

Zapata,

Olave

et al. 2024

Front. Bioeng. Biotechnol.

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“…Environments continuously exposed to elevated concentrations of heavy metals are known to promote metal tolerance in bacteria ( Clausen, 2000 ; Nosalova et al, 2023 ). The toxicity and solubility of metals is also known to be influenced by several physico-chemical parameters of that particular area as shown in Table 1 , which plays an important role in developing metal tolerance in the indigenous microbial population ( Shi et al, 2013 ; Gupta et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

“…Thus this interplay of several factors could have influenced the heavy metal availability and the degree of metal toxicity in the saltern, resulting in the emergence of the multi-metal tolerant Chromohalobacter sp. Nosalova et al (2023) in their study on mine tailings from a lead, silver and zinc mining heap noticed that the bacterial counts were low but showed exceptionally high levels of resistance to heavy metals. Our study showed a similar scenario as reported earlier ( Pereira et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

A halophilic Chromohalobacter species from estuarine coastal waters as a detoxifier of manganese, as well as a novel bio-catalyst for synthesis of n-butyl acetate

Pereira¹,

Kerkar

Dias³

et al. 2023

Front. Microbiol.

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Anthropogenic pollution due to ferro-manganese ore transport by barges through the Mandovi estuary in Goa, India is a major environmental concern. In this study a manganese (Mn) tolerant, moderately halophilic Chromohalobacter sp. belonging to the family Halomonadaceae was isolated from the sediments of a solar saltern adjacent to this Mandovi estuary. Using techniques of Atomic absorption spectroscopy, Scanning electron microscopy-Energy dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy and Atomic Force Microscopy, the Chromohalobacter sp. was explored for its ability to tolerate and immobilize Mn in amended and unamended media with 20% natural salt concentration (w/v). In aqueous media supplemented with 0.1 mM Mn, the Chromohalobacter sp. was capable of sequestering up to 76% Mn with an average immobilization rate of 8 mg Mn /g /day. Growth rate kinetic analysis using Gompertz mathematical functions was found to model the experimental data well. The model inferred that the maximum growth rate of Chromohalobacter sp. was at 10% natural salt concentration (w/v). The Chromohalobacter sp. was further found to be multimetal tolerant showing high tolerance to Iron (Fe), Nickel (Ni) and Cobalt (Co), (each at 4 mM), and tolerated Manganese (Mn) up to 6 mM. Morphologically, the Chromohalobacter sp. was a non-spore forming, Gram negative motile rod (0.726 μ× 1.33 μ). The adaptative mechanism of Chromohalobacter sp. to elevated Mn concentrations (1 mM) resulted in the reduction of its cell size to 0.339 μ× 0.997 μ and the synthesis of an extracellular slime, immobilizing Mn from the liquid phase forming Manganese oxide, as confirmed by Scanning Electron Microscopy. The expression of Mnx genes for manganese oxidation further substantiated the finding. This bacterial synthesized manganese oxide also displayed catalytic activity (∼50% conversion) for the esterification of butan-1-ol with CH3COOH to yield n-butyl acetate. This Chromohalobacter sp. being indigenous to marine salterns, has adapted to high concentrations of heavy metals and high salinities and can withstand this extremely stressed environment, and thus holds a tremendous potential as an environmentally friendly “green bioremediator” of Mn from euryhaline environments. The study also adds to the limited knowledge about metal-microbe interactions in extreme environments. Further, since Chromohalobacter sp. exhibits commendable catalytic activity for the synthesis of n-butyl acetate, it would have several potential industrial applications.

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Mining-related multi-resistance genes in sulfate-reducing bacteria treatment of typical karst nonferrous metal(loid) mine tailings in China

Liu,

Yao,

Zhou

et al. 2023

Environ Sci Pollut Res

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Diversity, heavy metals, and antibiotic resistance in culturable heterotrophic bacteria isolated from former lead–silver–zinc mine heap in Tarnowskie Gory (Silesia, Poland)

Cited by 6 publications

References 55 publications

MICP mediated by indigenous bacteria isolated from tailings for biocementation for reduction of wind erosion

MICP mediated by indigenous bacteria isolated from tailings for biocementation for reduction of wind erosion

A halophilic Chromohalobacter species from estuarine coastal waters as a detoxifier of manganese, as well as a novel bio-catalyst for synthesis of n-butyl acetate

Mining-related multi-resistance genes in sulfate-reducing bacteria treatment of typical karst nonferrous metal(loid) mine tailings in China

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