Diversity in aconitine alkaloid profile of Aconitum plants in Hokkaido contrasts with their genetic similarity

Kakiuchi, Nobuko; Atsumi, Toshiyuki; Higuchi, Mari; Kamikawa, Shohei; Miyako, Haruka; Wakita, Yuriko; Ohtsuka, Isao; Hayashi, Shigeki; Hishida, Atsuyuki; Kawahara, Nobuo; Nishizawa, Makoto; Yamagishi, Takashi; Kadota, Yasuhiro

doi:10.1007/s11418-014-0867-3

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…Aconite is used in Kampo medicine because of the deep influence by Shanghang Lun. Due to its toxicity, scientists analyzed the alkaloid content, nuclear DNA region, and internal transcribed spacer (ITS) from 107 Aconitum plants in Japan [67] and set the standard analytical method in the Japanese pharmacopeia [68]. The pharmacokinetics and toxicology of Aconitum preparations or powders were also studied [69,70].…”

Section: Aconite Preparations In Kampo Medicinementioning

confidence: 99%

Clinical Aspects of Aconitum Preparations

Tai

El-Shazly

et al. 2015

Planta Med

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Aconitum, also known as monkshood, wolfʼs bane, or devilʼs helmet, has been widely used in folk medicine in China, India, and certain parts of Europe [1-4]. The genus Aconitum (Ranunculaceae) comprises 300 species distributed all over the world [5]. The most common species are Aconitum carmichaelii Deb. and Aconitum kusnezoffii Rchb. in China, Aconitum japonicum Thunb. in Japan, Aconitum napellus L. in Europe, Aconitum ferox Wall. ex Ser. in India, and Aconitum noveboracense A. Gray ex Coville in the United States [5]. Several classes of secondary metabolites, especially alkaloids, have been isolated from different Aconitum sp. [6]. The type of the isolated major alkaloids may vary depending on the species such as aconitine, hypaconitine, and mesaconitine from A. carmichaelii, aconitine from A. napellus, hypaconitine from A. septentrionale Koelle, mesaconitine from A. kusnezoffii, bikhaconitine from A. ferox, talatisamine from A. kongboense Lauener, atisine from A. anthora L., and A. heterophyllum Wall. ex Royle, and lycaconitine from A. vulparia Rchb. [6]. Several isoquinoline alkaloids and phenethylamine derivatives have also been isolated, such as higenamine from A. japonicum, magnoflorine from A. vulparia and A. napellus, coryneine from A. carmichaelii, and N-methyl adrenaline from A. nasutum Fisch. ex Rchb. [6]. Lipo-alkaloids including lipoaconitines, lipomesaconitines, lipodeoxyaconitines, and lipohypaconitines were also isolated [7, 8]. The efficacy of Aconitum sp. in resolving critical clinical conditions has been proven by doctors practicing traditional Chinese medicine (TCM) and Ayurvedic medicine for centuries. However, the long history of Aconitum sp. misuse in homicide cases has shaken the faith in the potential safe application of this herb in therapy [9, 10]. The recent developments in analytical techniques which can identify and determine the concentrations of toxic compounds in herbal products with impressive accuracy and reliability have rekindled the interest in Aconitum preparations [1, 5, 6, 11, 12].

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Section: Aconite Preparations In Kampo Medicinementioning

confidence: 99%

Clinical Aspects of Aconitum Preparations

Tai

El-Shazly

et al. 2015

Planta Med

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“…In the case of accidental ingestion of toxic plants, rapid identification is important for emergency medical treatment. Aconitum plants, which belong to the family Ranunculaceae, comprise over 200 species worldwide, and approximately 20 species grow naturally in Japan [1–5]. The leaves of Aconitum plants are similar to those of edible plants, such as Anemone flaccida (Ranunculaceae) and Parasenecio delphiniifolius (Compositae), and often grow in the same habitat; therefore, accidental ingestion of Aconitum plants could occur [6].…”

Section: Introductionmentioning

confidence: 99%

Rapid identification of Aconitum plants based on loop-mediated isothermal amplification assay

et al. 2019

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Objective Aconitum plants (Ranunculaceae) exhibit toxicity, and accidental ingestion of the plants has been reported in Japan. Identifying the cause of poisoning is important for emergency medical treatment, and a rapid and simple detection technique is required for the identification of poisoning cause. In the present study, we developed a rapid and simple method for detecting Aconitum plant DNA using a loop-mediated isothermal amplification (LAMP) assay. Results Specific LAMP primers for Aconitum plants were designed based on the trnL – trnF intergenic spacer region. Using the LAMP primers, the LAMP assay included an initiation reaction of 10 min followed by amplification for 20 min at the isothermal reaction temperature of 65 °C. The LAMP reaction was demonstrated to be specific and highly sensitive to Aconitum plants, given that the assay can be used for 1 pg of purified DNA. Using raw extracted DNA as template, the entire detection procedure from DNA extraction to final detection required only 30 min. Moreover, the protocol identified samples containing approximately 5 mg of Aconitum plants cooked and digested with artificial gastric juice. The currently proposed protocol exhibits good potential as a screening method of Aconitum plant poisoning for emergency medical care. Electronic supplementary material The online version of this article (10.1186/s13104-019-4463-1) contains supplementary material, which is available to authorized users.

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