Diversity in Endophyte Populations Reveals Functional and Taxonomic Diversity between Wild and Domesticated Grapevines

Campisano, A.; Pancher, Michael; Puopolo, Gerardo; Puddu, Antonio; López-Fernández, Sebastián; Biagini, B.; Yousaf, Sohail; Pertot, I.

doi:10.5344/ajev.2014.14046

Cited by 47 publications

(48 citation statements)

References 37 publications

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“…Our data also confirm the existence of a metabolic signature associated with the inoculation of grapevine with strain Enterobacter ludwigii EnVs6. Previously, this strain was characterized in terms of plant growth promotion properties, antibiotic resistance, quorum sensing activity, enzyme production and biocontrol against grapevine pathogens, performing very well as compared to a collection of endophytic bacteria (Campisano et al 2014b). We chose strain EnVs6 for our experiments since analysis of plant protection properties have shown a remarkable performance as a biocontrol agent and as a plant growth promoter.…”

Section: Discussionmentioning

confidence: 99%

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Grapevine colonization by endophytic bacteria shifts secondary metabolism and suggests activation of defense pathways

et al. 2015

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Aims The colonization pattern of three grapevine endop h y t e s ( f a m i l i e s S p h i n g o m o n a d a c e a e a n d Enterobacteriaceae) and their putative metabolic signature in plants were analyzed on Vitis vinifera L. cv. Pinot noir to determine the behavior of endophytic strains inside plants as well as how plants respond to such microsymbionts. Methods Strains Enterobacter ludwigii EnVs6, Pantoea vagans PaVv7 and Sphingomonas phyllosphaerae SpVs6, were root inoculated on micropropagated grapevine plantlets and colonization was determined by double labeling of oligonucleotide probes-fluorescence in situ hybridization (DOPE-FISH) coupled with confocal microscopy. After inoculation, the metabolic signature in plants colonized by Enterobacter ludwigii EnVs6 was further studied using UPLC//tandem mass spectrometry analysis.Results E. ludwigii EnVs6 and P. vagans PaVv7 colonized the plantlets and were both observed on the root surfaces and as endophytes in the cortex and inside the central cylinder up to xylem vessels, but not in the systemic plant parts. Strain SpVs6 also efficiently colonized the root surface, but not the endorhiza and was therefore not detected as an endophyte. A metabolic signature in plants inoculated with E. ludwigii EnVs6 was depicted, resulting in a significant increase in vanillic acid and a decrease in the concentration of catechin, esculin, arbutin, astringin, pallidol, ampelopsin, D-quadrangularin and isohopeaphenol. Changes in the concentration of epicatechin, procyanidin 1, taxifolin and the sum of quercetin-3-glucoside and quercetin-3-galactoside, in roots and stems were also detected, showing that the effect of colonization of plants is most prominent in the stems. Conclusions Colonization patterns in endophytes are divergent according to the strains used. A metabolic signature suggests the activation of pathways involved in plant defense but also modulation of the production of metabolites that are keys for colonization.

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Section: Discussionmentioning

confidence: 99%

“…sylvestris (EnVs6 and SpVs6) and V. vinifera L. cv. vinifera (PaVv7) and characterized previously (Campisano et al 2014b). Bacteria were grown in LB broth at 28°C with shaking (180 r.p.m.)…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Grapevine colonization by endophytic bacteria shifts secondary metabolism and suggests activation of defense pathways

et al. 2015

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“…Furthermore, Pseudomonas sp. (72,73), Enterobacteriaceae sp., and many other endophytic grapevine bacteria (26) are able to produce IAA. Interactions of nonpathogenic with pathogenic bacteria are known for tumors of olive trees, induced by Pseudomonas savastanoi (pv.…”

Section: Discussionmentioning

confidence: 99%

“…Techniques such as isolation of cultivable bacteria (26), in combination with analysis of fluorescently labeled terminal-restriction fragment analysis (27) and taxon-or genus-specific real-time PCR (28), have been used. The 16S rRNA gene amplicon high-throughput sequencing technique provides a detailed overview of the microbiota and has been employed to resolve bacterial communities to the species level (29).…”

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Grapevine (Vitis vinifera) Crown Galls Host Distinct Microbiota

Faist¹,

Keller

Hentschel³

et al. 2016

Appl Environ Microbiol

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Crown gall disease of grapevine is caused by virulent Agrobacterium strains and establishes a suitable habitat for agrobacteria and, potentially, other bacteria. The microbial community associated with grapevine plants has not been investigated with respect to this disease, which frequently results in monetary losses. This study compares the endophytic microbiota of organs from grapevine plants with or without crown gall disease and the surrounding vineyard soil over the growing seasons of 1 year. Amplicon-based community profiling revealed that the dominating factor causing differences between the grapevine microbiota is the sample site, not the crown gall disease. The soil showed the highest microbial diversity, which decreased with the distance from the soil over the root and the graft union of the trunk to the cane. Only the graft union microbiota was significantly affected by crown gall disease. The bacterial community of graft unions without a crown gall hosted transient microbiota, with the three most abundant bacterial species changing from season to season. In contrast, graft unions with a crown gall had a higher species richness, which in every season was dominated by the same three bacteria (Pseudomonas sp., Enterobacteriaceae sp., and Agrobacterium vitis). For in vitro-cultivated grapevine plantlets, A. vitis infection alone was sufficient to cause crown gall disease. Our data show that microbiota in crown galls is more stable over time than microbiota in healthy graft unions and that the microbial community is not essential for crown gall disease outbreak. IMPORTANCEThe characterization of bacterial populations in animal and human diseases using high-throughput deep-sequencing technologies, such as 16S amplicon sequencing, will ideally result in the identification of disease-specific microbiota. We analyzed the microbiota of the crown gall disease of grapevine, which is caused by infection with the bacterial pathogen Agrobacterium vitis. All other Agrobacterium species were found to be avirulent, even though they lived together with A. vitis in the same crown gall tumor. As has been reported for human cancer, the crown gall tumor also hosted opportunistic bacteria that are adapted to the tumor microenvironment. Characterization of the microbiota in various diseases using amplicon sequencing may help in early diagnosis, to serve as a preventative measure of disease in the future.

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“…Endophytes are microorganisms living, at least a part of their life cycle, within plant tissues without causing disease (Hardoim et al, 2015). In the grapevine they respond to plant physiology and the environment, including the cropping system, and they may play a role in plant resilience or as source of producers of bioactive metabolites (Pancher et al, 2012;Campisano et al, 2014Campisano et al, , 2015. Although at the moment the employment of the grapevine microbiome is at its real infancy, great promises are posed in the use of the most advanced technologies for the discovery of the next generation of microbial biofungicides, as for example new microbial antagonists of pathogens, bioactive compounds produced by endophytes or approaches to improve the efficacy of the biocontrol microorganisms (Massart et al, 2015).…”

Section: Leaf and Bunch Diseasementioning

confidence: 99%

A critical review of plant protection tools for reducing pesticide use on grapevine and new perspectives for the implementation of IPM in viticulture

Pertot¹,

et al. 2017

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a b s t r a c tSeveral pests and diseases have grapevine as their favourite host and the vineyard as preferred environment, so an intensive pesticide schedule is usually required to meet qualitative and quantitative production standards. The need to prevent the negative impact of synthetic chemical pesticides on human health and the environment and the consumer expectations in term of chemical residues in food stimulated the research of innovative tools and methods for sustainable pest management. The research project PURE (www.pure-ipm.eu) was a Europe-wide framework, which demonstrated that several solutions are now available for the growers and evaluated several new alternatives that are under development or almost ready for being applied in practice. Although the use of resistant/tolerant varieties is not yet feasible in several traditional grape growing areas, at least part of the synthetic chemical pesticides can be substituted with biocontrol agents to control pests and pathogens and/or pheromone mating disruption, or the number of treatments can be reduced by the use of decision support systems, which identify the optimal timing for the applications. This review presents the state of the art and the perspectives in the field of grapevine protection tools and strategies.

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Diversity in Endophyte Populations Reveals Functional and Taxonomic Diversity between Wild and Domesticated Grapevines

Cited by 47 publications

References 37 publications

Grapevine colonization by endophytic bacteria shifts secondary metabolism and suggests activation of defense pathways

Grapevine colonization by endophytic bacteria shifts secondary metabolism and suggests activation of defense pathways

Grapevine (Vitis vinifera) Crown Galls Host Distinct Microbiota

A critical review of plant protection tools for reducing pesticide use on grapevine and new perspectives for the implementation of IPM in viticulture

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