Diversity in mRNA expression of the serine-type carboxypeptidase <i>ocpG</i> in <i>Aspergillus oryzae</i> through intron retention

Ishida, Ken; Kuboshima, Megumi; Morita, Hiroto; Maéda, Hiroshi; Okamoto, Ayako; Takeuchi, Michio; Yamagata, Yuriko

doi:10.1080/09168451.2014.923291

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…An increasing research has demonstrated that intron retention is widely found in animals, plants and fungi, serving as one of the effective strategies for post transcriptional regulation in eukaryotes [ 5 ]. In fungi, IR is reported to be involved in fungal cell complexity, pathogenicity [ 7 ], heat shock response [ 8 ], the nutrient sensing, such as glucose [ 9 ], and nitrogen source [ 10 ]. However, the function of IR in fungi has not been well studied, as compared to the extensive study of IR in animals and plants.…”

Section: Introductionmentioning

confidence: 99%

Intron retention coupled with nonsense-mediated decay is involved in cellulase biosynthesis in cellulolytic fungi

Gao

Pang

et al. 2022

Biotechnol Biofuels

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Background Knowledge on regulatory networks associated with cellulase biosynthesis is prerequisite for exploitation of such regulatory systems in enhancing cellulase production with low cost. The biological functions of intron retention (IR) and nonsense-mediated mRNA decay (NMD) in filamentous fungi is lack of study, let alone their roles in cellulase biosynthesis. Results We found that major cellulase genes (cel7a, cel7b, and cel3a) exhibited concomitant decrease in IR rates and increase in their gene expression in T. reesei under cellulase-producing condition (cellulose and lactose) that was accompanied with a more active NMD pathway, as compared to cellulase non-producing condition (glucose). In the presence of the NMD pathway inhibitor that successfully repressed the NMD pathway, the mRNA levels of cellulase genes were sharply down-regulated, but the rates of IR in these genes were significantly up-regulated. Consistently, the cellulase activities were severely inhibited. In addition, the NMD pathway inhibitor caused the downregulated mRNA levels of two important genes of the target of rapamycin (TOR) pathway, trfkbp12 and trTOR1. The absence of gene trfkbp12 made the cellulase production in T. reesei more sensitive to the NMD pathway inhibitor. Conclusions All these findings suggest that the IR of cellulase genes regulates their own gene expression by coupling with the NMD pathway, which might involve the TOR pathway. Our results provide better understanding on intron retention, the NMD pathway, and cellulase production mechanism in filamentous fungi.

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Section: Introductionmentioning

confidence: 99%

Intron retention coupled with nonsense-mediated decay is involved in cellulase biosynthesis in cellulolytic fungi

Gao

Pang

et al. 2022

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…An increasing research has demonstrated that intron retention is widely found in animals, plants and fungi, serving as one of the effective strategies for post transcriptional regulation in eukaryotes [5]. In fungi, IR is reported to be involved in fungal cell complexity, pathogenicity [7], heat shock response [8], the nutrient sensing such as glucose [9], and nitrogen source [10]. However, the function of IR in fungi has not been well studied, as compared to the extensive study of IR in animals and plants.…”

Section: Introductionmentioning

confidence: 99%

Intron Retention Coupled with Nonsense-Mediated Decay is Involved in Cellulase Biosynthesis in Cellulolytic Fungi

Gao

Pang

et al. 2022

Preprint

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Background Knowledge on regulatory networks associated with cellulase biosynthesis is prerequisite for exploitation of such regulatory systems in ehancing cellulase production with low cost. The biological functions of intron retention (IR) and nonsense-mediated mRNA decay (NMD) in filamentous fungi is lack of study, let alone their roles in cellulase biosynthesis. Result We found that major cellulase genes (cel7a, cel7b, and cel3a) exhibited concomitant decrease in IR rates and increase in their gene expression in T. reesei under cellulase-producing condition (cellulose and lactose) that was accompanied with a more active NMD pathway, as compared to non cellulase-producing condition (glucose). In the presence of the NMD pathway inhibitor that successfully repressed the NMD pathway, the mRNA levels of cellulase genes were sharply down-regulated, but the rates of IR in these genes were significantly up-regulated. Consistently, the cellulase activities were severely inhibited. In addition, the NMD pathway inhibitor caused the downregulated mRNA levels of two important genes of the target of rapamycin (TOR) pathway, trfkbp12 and trTOR1. The absence of gene trfkbp12 made the cellulase production in T. reesei more sensitive to the NMD pathway inhibitor. Conclusion All these findings suggest that the IR of cellulase genes regulates their own gene expression by coupling with the NMD pathway, which might involve the TOR pathway. Our results provide better understanding on intron retention, the NMD pathway, and cellulase production mechanism in filamentous fungi.

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Three extracellular dipeptidyl peptidases found in Aspergillus oryzae show varying substrate specificities

Maéda

Sakai

Kobayashi

et al. 2016

Appl Microbiol Biotechnol

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Three extracellular dipeptidyl peptidase genes, dppB, dppE, and dppF, were unveiled by sequence analysis of the Aspergillus oryzae genome. We investigated their differential enzymatic profiles, in order to gain an understanding of the diversity of these genes. The three dipeptidyl peptidases were expressed using Aspergillus nidulans as the host. Each recombinant enzyme was purified and subsequently characterized. The enzymes displayed similar optimum pH values, but optimum temperatures, pH stabilities, and substrate specificities varied. DppB was identified as a Xaa-Prolyl dipeptidyl peptidase, while DppE scissile substrates were similar to the substrates for Aspergillus fumigatus DPPV (AfDPPV). DppF was found to be a novel enzyme that could digest both substrates for A. fumigatus DPPIV and AfDPPV. Semi-quantitative PCR revealed that the transcription of dppB in A. oryzae was induced by protein substrates and repressed by the addition of an inorganic nitrogen source, despite the presence of protein substrates. The transcription of dppE depended on its growth time, while the transcription of dppF was not affected by the type of the nitrogen source in the medium, and it started during the early stage of the fungal growth. Based on these results, we conclude that these enzymes may represent the nutrition acquisition enzymes. Additionally, DppF may be one of the sensor peptidases responsible for the detection of the protein substrates in A. oryzae environment. DppB may be involved in nitrogen assimilation control, since the transcription of dppB was repressed by NaNO3, despite the presence of protein substrates.

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Diversity in mRNA expression of the serine-type carboxypeptidase ocpG in Aspergillus oryzae through intron retention

Abstract: (2014) Diversity in mRNA expression of the serine-type carboxypeptidase ocpG in Aspergillusoryzae through intron retention, Bioscience, Biotechnology, and Biochemistry, 78:8, 1328-1336,

Cited by 5 publications

References 35 publications

Intron retention coupled with nonsense-mediated decay is involved in cellulase biosynthesis in cellulolytic fungi

Intron retention coupled with nonsense-mediated decay is involved in cellulase biosynthesis in cellulolytic fungi

Intron Retention Coupled with Nonsense-Mediated Decay is Involved in Cellulase Biosynthesis in Cellulolytic Fungi

Three extracellular dipeptidyl peptidases found in Aspergillus oryzae show varying substrate specificities

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