Diversity in sea buckthorn (Hippophae rhamnoides L.) accessions with different origins based on morphological characteristics, oil traits, and microsatellite markers

Abstract

Sea buckthorn (Hippophae rhamnoides) is an ecologically and economically important species. Here, we assessed the diversity of 78 accessions cultivated in northern China using 8 agronomic characteristics, oil traits (including oil content and fatty acid composition) in seeds and fruit pulp, and SSR markers at 23 loci. The 78 accessions included 52 from ssp. mongolica, 6 from ssp. sinensis, and 20 hybrids. To assess the phenotypic diversity of these accessions, 8 agronomic fruit traits were recorded and analyzed using principal component analysis (PCA). The first two PCs accounted for approximately 78% of the variation among accessions. The oil contents were higher in pulp (3.46-38.56%) than in seeds (3.88-8.82%), especially in ssp. mongolica accessions. The polyunsaturated fatty acid (PUFA) ratio was slightly lower in the seed oil of hybrids (76.06%) than that of in ssp. mongolica (77.66%) and higher than that of in ssp. sinensis (72.22%). The monounsaturated fatty acid (MUFA) ratio in the pulp oil of ssp. sinensis (57.00%) was highest, and that in ssp. mongolica (51.00%) was equal to the ratio in the hybrids (51.20%). Using canonical correspondence analysis (CCA), we examined the correlation between agronomic traits and oil characteristics in pulp and seeds. Oil traits in pulp from different origins were correlated with morphological groupings (r = 0.8725, p = 0.0000). To assess the genotypic diversity, 23 SSR markers (including 17 loci previously reported) were used among the 78 accessions with 59 polymorphic amplified fragments obtained and an average PIC value of 0.2845. All accessions were classified into two groups based on the UPGMA method. The accessions of ssp. sinensis and ssp. mongolica were genetically distant. The hybrid accessions were close to ssp. mongolica accessions. The 8 agronomic traits, oil characteristics in seed and pulp oils, and 23 SSR markers successfully distinguished the 78 accessions. These results will be valuable for cultivar identification and genetic diversity analysis in cultivated sea buckthorn.

Sea Buckthorn Leaf Powders: The Impact of Cultivar and Drying Mode on Antioxidant, Phytochemical, and Chromatic Profile of Valuable Resource

“…The HR plants can withstand a great range of temperatures and they are resistant to drought. Moreover, the HR plants can easily develop a complex root system coupled to nitrogen fixation and be used for soil erosion prevention or be suitable for planting in degraded soils [ 3 , 6 ]. All parts of HR plants and their extracts can be used for pharmaceutical, nutraceutical, cosmetical, food, and fodder purposes, with the most well-known materials being fruits and leaves [ 4 , 5 , 7 , 8 ].…”

Genomic SSR and EST‐SSR markers for phylogenetic and pedigree reconstructions—A comparison in sea buckthorn

“…H. rhamnoides has high ecological, medicinal and economic value (Bal et al, 2011; Dhyani et al, 2011; Jadhav, 2014; Kortesniemi et al., 2017; Suryakumar & Gupta, 2011), and thus has attracted an abundance of research on breeding, systematics, biochemistry and genetics (Bartish, 2016). Previous studies have developed SSR markers for sea buckthorn from enriched genomic libraries (Islam et al, 2015; Wang et al., 2008) and transcriptome sequences (Jain et al., 2014; Li et al, 2020; Li, Ruan, et al, 2017; Li, Zeng, et al., 2017; Xie et al., 2017). Few has discussed the usage of SSR markers on sea buckthorn phylogenetic analysis and/or breeding programme.…”

Inclusion Complex of Sea Buckthorn Fruit Oil with β‐Cyclodextrin: Preparation Characterization and Antioxidant Activity

“…The POA content may differ in the extraction method and production location. [ 29 ] The 11,14,17‐eicosatrienoic acid content analyzed in this experiment was much higher than in previous literature. [ 9 ] These findings laid the foundation for subsequent development and utilization.…”