Diversity of actinomycetes isolated from Challenger Deep sediment (10,898 m) from the Mariana Trench

Abstract

Thirty-eight actinomycetes were isolated from sediment collected from the Mariana Trench (10,898 m) using marine agar and media selective for actinomycetes, notably raffinose-histidine agar. The isolates were assigned to the class Actinobacteria using primers specific for members of this taxon. The phylogenetic analysis based on 16S rRNA gene sequencing showed that the isolates belonged to the genera Dermacoccus, Kocuria, Micromonospora, Streptomyces, Tsukamurella and Williamsia. All of the isolates were screened for genes encoding nonribosomal peptide and polyketide synthetases. Nonribosomal peptide synthetase sequences were detected in more than half of the isolates and polyketide synthases type I (PKS-I) were identified in five out of 38 strains. The Streptomyces isolates produced several unusual secondary metabolites, including a PKS-I associated product. In initial testing for piezotolerance, the Dermacoccus strain MT1.1 grew at elevated hydrostatic pressures.

In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus

“…Due to their widespread distribution and remarkable capacity to produce a wide range of bioactive compounds [16], Streptomyces species have attracted considerable commercial interest. Soil streptomycetes are major producers of bioactive secondary metabolites [17][18][19][20].…”

Characterization of cytotoxic compound from marine sediment derived actinomycete Streptomyces avidinii strain SU4

“…Streptomyces species are distributed widely in marine and terrestrial habitats [12] and are of commercial interest due to their unique capacity to produce novel metabolites.…”

Actinomycetes of Orthosipon stamineus rhizosphere as producer of antibacterial compound against multidrug resistant bacteria

“…Beside, this bacteria is also the main microbia in micro-soil ecosystem [4] and it is the source of bioactive compound which is widely distributed in marine and terrestrial habitate [5]. Actinomycetes is abundant surrounds the high level plant rooting [6] and in rhizosphere soil approaching 40% in the total of soil microflora.…”