Diversity of adsorption affinity and catalytic activity of fungal phosphatases adsorbed on some tropical soils

Kedi, Brice; Abadie, Josiane; Sei, Joseph; Quiquampoix, Hervé; Staunton, Siobhán

doi:10.1016/j.soilbio.2012.02.006

Cited by 22 publications

(13 citation statements)

References 37 publications

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“…These results support findings from studies of purified, single enzymes finding that a pH value that maximizes substrate–enzyme collision (i.e., K m pH optimum) is not necessarily the pH condition at which catalysis is greatest (i.e., V max pH optimum) (Hansen & Raines, 1990; Kedi et al., 2013; Wang et al., 1995). Though the apparent kinetic properties for soil enzymes represent the sum of multiple isozymes and/or pools of varying organomineral associations (Burns et al., 2013), purified enzymes have also been found to exhibit decoupled pH optima of V max and K m (Andrews & Pallavicini, 1973; Chaudhuri et al., 2013; Fernley & Walker, 1965).…”

Section: Resultssupporting

confidence: 84%

Apparent kinetic properties of soil phosphomonoesterase and β‐glucosidase are disparately influenced by pH

Margenot

2021

Soil Science Soc of Amer J

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Extracellular enzymes catalyze organic matter transformations in soils, yet the study of soil enzymes does not typically evaluate how enzyme kinetic properties that govern activity in situ may shift with environmental parameters such as pH. To test this, we quantified changes in apparent kinetic properties (maximum catalysis rate [AppVmax] and substrate concentration that produces 50% of Vmax [AppKm]) of soil phosphomonoesterase (PME) and β‐glucosidase (BG) as a function of pH by using dual gradients of buffer pH (3–12) and substrate concentrations in a selected set of diverse soils. A third kinetic property, apparent specificity constant (AppKa = AppVmax/AppKm) was also evaluated as a function of pH. Results indicated widely differing pH optima of soil PME and BG AppVmax and AppKm, decoupling of which entailed unpredictable pH optima of AppKa. Additionally, changes in soil enzyme activity with pH may not be driven by shifts in the specificity constant because pH–AppVmax relationships are largely unrelated to co‐occurring changes in AppKa. These results invalidate the assumption that Km of a soil enzyme is constant across pH and emphasize the importance of assaying soil enzyme activities at sufficiently high (5 × Km) substrate concentrations at a given pH to avoid the mischaracterizing pH optima.

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Section: Resultssupporting

confidence: 84%

Apparent kinetic properties of soil phosphomonoesterase and β‐glucosidase are disparately influenced by pH

Margenot

2021

Soil Science Soc of Amer J

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“…In addition to that, the same functional-enzyme type can be produced by different microorganisms or strains of a particular microorganism [55]. Thus, the same EEs from different sources could differ in their response to the environmental conditions or in their affinity for surfaces [24,56]. For instance, Kedi, et al [56] studied the adsorption of three phosphatases produced from two strains Hebeloma cylindrosporum and one Suillus collinitus in tropical soils.…”

Section: Potential Microbial Adaptation To Reduced Potential Enzyme Amentioning

confidence: 99%

“…Thus, the same EEs from different sources could differ in their response to the environmental conditions or in their affinity for surfaces [24,56]. For instance, Kedi, et al [56] studied the adsorption of three phosphatases produced from two strains Hebeloma cylindrosporum and one Suillus collinitus in tropical soils. Their results showed diversities in the adsorption of the two fungi phosphatases on soils with a considerable divergence between the enzymes.…”

Section: Potential Microbial Adaptation To Reduced Potential Enzyme Amentioning

confidence: 99%

Control of Soil Extracellular Enzyme Activities by Clay Minerals—Perspectives on Microbial Responses

2019

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Knowledge of how interactions of clay minerals and extracellular enzymes (EEs) influence organic matter turnover in soils are still under discussion. We studied the effect of different montmorillonite contents on EE activities, using two experiments—(1) an adsorption experiment with a commercially available enzyme (α-glucosidase) and (2) an incubation experiment (10 days) where microorganisms were stimulated to produce enzymes through organic carbon (OC) addition (starch and cellulose). Soil mixtures with different montmorillonite contents were created in four levels to a sandy soil: +0% (control), +0.1%, +1%, and +10%. The potential enzyme activity (pEA) of four enzymes, α-glucosidase, β-glucosidase, cellobiohydrolase, and aminopeptidase, involved in the soil carbon and nitrogen cycle were analysed. The adsorption experiment revealed a reduction in the catalytic activity of α-glucosidase by up to 76% with increasing montmorillonite contents. However, the incubation experiment showed an inhibitory effect on pEA only directly after the stimulation of in-situ EE production by OC addition. At later incubation stages, higher pEA was found in soils with higher montmorillonite contents. This mismatch between both experiments, with a transient reduction in catalytic activity for the incubation experiments, points to the continuous production of enzymes by soil microorganisms. It is conceivable that microbial adaptation is characterized by higher investment in EEs production induced by increasing clay contents and a stabilisation of the EEs by clay minerals. Our results point to the need to better understand EE-clay mineral-OC interactions regarding potential microbial adaptations and EE stabilisation with potentially prolonged activities.

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“…The negative correlation between available P content and MAP likely originates from the increasing P sorption by Fe and Al oxyhydroxides resulting from higher weathering rates and silica leaching (Schaefer et al, 2008). Also, the decomposition of fresh and complex organic matters is carried out by extracellular enzymes whose activities strongly depend positively or negatively on pH, especially in Malagasy Ferralsols (Kedi et al, 2013). And pH in highly weathered soil tends also to increase to values close to the zero-point charge of Fe and Al oxides (between 6 and 7; Schaefer et al, 2008).…”

Section: Climatic Determinants Of C Mineralization Activitiesmentioning

confidence: 99%

Soil microbial diversity drives the priming effect along climate gradients: a case study in Madagascar

Razanamalala¹,

Razafimbelo²,

Maron³

et al. 2017

The ISME Journal

128

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The priming effect in soil is proposed to be generated by two distinct mechanisms: 'stoichiometric decomposition' and/or 'nutrient mining' theories. Each mechanism has its own dynamics, involves its own microbial actors, and targets different soil organic matter (SOM) pools. The present study aims to evaluate how climatic parameters drive the intensity of each priming effect generation mechanism via the modification of soil microbial and physicochemical properties. Soils were sampled in the center of Madagascar, along climatic gradients designed to distinguish temperature from rainfall effects. Abiotic and biotic soil descriptors were characterized including bacterial and fungal phylogenetic composition. Potential organic matter mineralization and PE were assessed 7 and 42 days after the beginning of incubation with C-enriched wheat straw. Both priming mechanisms were mainly driven by the mean annual temperature but in opposite directions. The priming effect generated by stoichiometric decomposition was fostered under colder climates, because of soil enrichment in less developed organic matter, as well as in fast-growing populations. Conversely, the priming effect generated by nutrient mining was enhanced under warmer climates, probably because of the lack of competition between slow-growing populations mining SOM and fast-growing populations for the energy-rich residue entering the soil. Our study leads to hypotheses about the consequences of climate change on both PE generation mechanisms and associated consequences on soil carbon sequestration.

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Diversity of adsorption affinity and catalytic activity of fungal phosphatases adsorbed on some tropical soils

Cited by 22 publications

References 37 publications

Apparent kinetic properties of soil phosphomonoesterase and β‐glucosidase are disparately influenced by pH

Apparent kinetic properties of soil phosphomonoesterase and β‐glucosidase are disparately influenced by pH

Control of Soil Extracellular Enzyme Activities by Clay Minerals—Perspectives on Microbial Responses

Soil microbial diversity drives the priming effect along climate gradients: a case study in Madagascar

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