Diversity of Aerobic Methanotrophic Bacteria in a Permafrost Active Layer Soil of the Lena Delta, Siberia

Liebner, Susanne; Rublack, Katja; Stuehrmann, Torben; Wagner, Dirk

doi:10.1007/s00248-008-9411-x

Cited by 98 publications

(89 citation statements)

References 57 publications

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“…Another study on Siberia showed the same results to our study that type II methanotrophs were not detected, and similarly, only two genera, Methylobacter and Methylosarcina were presented in the study. And within these two genera, Methylobacter was dominated (Liebner et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

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Diversity of methanotrophs in Zoige wetland soils under both anaerobic and aerobic conditions

Yun

et al. 2010

Journal of Environmental Sciences

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Zoige wetland is one of the most important methane emission centers in China. The oxidation of methane in the wetland affects global warming, soil ecology and atmospheric chemistry. Despite their global significance, microorganisms that consume methane in Zoige wetland remain poorly characterized. In this study, we investigated methanotrophs diversity in soil samples from both anaerobic site and aerobic site in Zoige wetland using pmoA gene as a molecular marker. The cloning library was constructed according to the pmoA sequences detected. Four clusters of methanotrophs were detected. The phylogenetic tree showed that all four clusters detected were affiliated to type I methanotrophs. Two novel clusters (cluster 1, cluster 2) were found to relate to none of the recognized genera of methanotrophs. These clusters have no cultured representatives and reveal an ecological adaptation of particular uncultured methanotrophs in Zoige wetland. Two clusters were belonging to Methylobacter and Methylococcus separately. Denaturing gradient gel electrophoresis gel bands pattern retrieved from these two samples revealed that the community compositions of anaerobic soil and aerobic soil were different from each other while anaerobic soil showed a higher metanotrophs diversity. Real-time PCR assays of the two samples demonstrated that aerobic soil sample in Zoige wetland was 1.5 times as much copy numbers as anaerobic soil. These data illustrated that methanotrophs are a group of microorganisms influence the methane consumption in Zoige wetland.

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Section: Discussionmentioning

confidence: 99%

“…The reason to the unsuccessfully amplification of type II could be due to the bias of PCR. In the procedure of PCR, type II was artificially biased owning to the low abundance in our samples (Liebner et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Diversity of methanotrophs in Zoige wetland soils under both anaerobic and aerobic conditions

Yun

et al. 2010

Journal of Environmental Sciences

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“…Chelatococcus, classified as an aerobic methanogenic bacterium, belongs to an alphaproteobacterial family that can grow on a wide range of substrates (Liebner et al 2009). This organism may be a promising source of IL tolerant cellulases due to high microbial activity detected while in the presence of tetra butyl IL.…”

Section: Discussionmentioning

confidence: 99%

Enrichment of microbial communities tolerant to the ionic liquids tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate

Pace

Ceballos

Harrold

et al. 2016

Appl Microbiol Biotechnol

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Aims: The aims of this study were to identify thermophilic microbial communities that degrade green waste in the presence of the ionic liquids (IL) tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate and examine preservation methods for IL tolerant communities.Methods and Results: High-solids incubations with step-wise increases in IL concentration were conducted to enrich for thermophilic IL tolerant communities. 16S rRNA sequencing of enriched communities revealed microorganisms capable of tolerating high levels of IL. Cryogenic preservation of enriched communities reduced the IL tolerance of the community and decreased the relative abundance of IL tolerant organisms. The use of cryoprotectants did not have an effect on microbial activity on green waste of the stored community. Conclusions:Step-wise increases in IL facilitated the enrichment of thermophilic, IL tolerant microbial communities that decomposed green waste. Alternative community storage and revival methods are necessary for maintenance and recovery of IL-tolerant communities.Significance and Impact of Study: A successful approach was developed to enrich communities that decompose green waste in thermophilic high-solids environments in the presence of IL. The enriched communities provide a targeted source of enzymes for the bioconversion of IL-pretreated green waste for conversion to biofuels.

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“…In the same area, specific clusters of methane oxidizing Proteobacteria closely related to Methylobacter psychrophilus and to Methylobacter tundripaludum were detected indicating a micro-diverse community on the species level (Liebner et al 2008). Also, highly divergent functional gene sequences of these methanotrophs were found in soils of the high Canadian Arctic (Pacheco-Oliver et al 2002).…”

Section: Methane Oxidizing Proteobacteriamentioning

confidence: 95%

“…In contrast, the population structure of methanogenic archaea in a permafrost affected peat in Siberia remained constant over a wide temperature range (Metje and Frenzel 2007). Also, a psychrophilic and little diverse methanotrophic community as detected near the permafrost table of Siberian polygonal tundra soils (Liebner and Wagner 2007;Liebner et al 2008) will likely require more time for resilience than the diverse mesophilic-psychrotolerant methanogenic community detected in permafrost soils of the same region (Ganzert et al 2006).…”

Section: Methane-cycling Communities Under Global Climate Changementioning

confidence: 99%

Global Warming and Carbon Dynamics in Permafrost Soils: Methane Production and Oxidation

Wagner

Liebner

Soil Biology

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The Arctic plays a key role in the Earth's climate system, because global warming is predicted to be most pronounced at high latitudes, and one third of the global carbon pool is stored in ecosystems of the northern latitudes. The degradation of permafrost and the associated intensified release of methane, a climate-relevant trace gas, represent potential environmental hazards. The microorganisms driving methane production and oxidation in Arctic permafrost soils have remained poorly investigated. Their population structure and reaction to environmental change is largely unknown, which means that also an important part of the process knowledge on methane fluxes in permafrost ecosystems is far from completely understood. This hampers prediction of the effects of climate warming on arctic methane fluxes. Further research on the stability of the methane cycling communities is therefore highly important for understanding the effects of a warming Arctic on the global climate. This review first examines the methane cycle in permafrost soils and the involved microorganisms. It then describes some aspects of the potential impact of global warming on the methanogenic and methanotrophic communities.

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Diversity of Aerobic Methanotrophic Bacteria in a Permafrost Active Layer Soil of the Lena Delta, Siberia

Cited by 98 publications

References 57 publications

Diversity of methanotrophs in Zoige wetland soils under both anaerobic and aerobic conditions

Diversity of methanotrophs in Zoige wetland soils under both anaerobic and aerobic conditions

Enrichment of microbial communities tolerant to the ionic liquids tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate

Global Warming and Carbon Dynamics in Permafrost Soils: Methane Production and Oxidation

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