Rhopalaea bilobata, a new species of the class Ascidiacea, under the family Diazonidae has been described from the Andaman Islands of Andaman & Nicobar, India. The species was found in sandy bottoms, reef areas and artificial structures at a depth range of 10–40 m. This species has bilobed anal border, six lobed branchial and atrial siphons, transparent thorax with pigmented spots at the anus, between the two siphons and at the anterior end of endostyle, and ramified basal test. The species is closely related to R. idoneta, R. macrothorax and R. tenuis in several sets of anatomical and morphological features.