Diversity of Bacillus-like organisms isolated from deep-sea hypersaline anoxic sediments

Saß, Andrea; McKew, Boyd A.; Sass, Henrik; Fichtel, Jörg; Timmis, Kenneth N.; McGenity, Terry J.

doi:10.1186/1746-1448-4-8

Cited by 63 publications

(65 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…30% along the transition from the upper to the lower halocline in Discovery (Figure 4). Moreover, OTU1288 is very closely associated (Table S3) to a Clostridium--like phylotype, found previously in the L'Atalante basin (Sass et al 2008). These observations suggest that these two OTUs may represent potentially active populations in the halocline sediments.…”

Section: Methodssupporting

confidence: 72%

“…the Burkholderiales, but are mostly associated with soils. Representatives of some bacteria not frequently found in typical marine environments that have been found in other DHAB waters include sequences affiliated with the genus Bacillus from the Urania chemocline and brine water column (Sass et al 2008). Surface sediments underlying DHAB brines, at least in terms of water activity and water potential (i.e.…”

Section: Methodsmentioning

confidence: 99%

“…One study examined diversity of Bacteria and Archaea in sediments of the Medee basin (Akoumianaki et al 2012) and another study presented information on bacterial isolates generated from Mediterranean Sea DHAB sediments (Sass et al 2008). A more recent study, conducted in parallel with the investigation presented in this manuscript, focused on protist and fungal assemblages present in the sediments underlying the haloclines of Discovery, Urania and L'Atalante basins (Bernhard et al 2014).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins

et al. 2015

View full text Add to dashboard Cite

The sediment microbiota of the Mediterranean deep--sea anoxic hypersaline basins (DHABs) are understudied relative to communities in the brines and halocline waters. In this study, the active fraction of the prokaryotic community in the halocline sediments of L' Atalante, Urania and Discovery DHABs was investigated based on extracted total RNA and 454 pyrosequencing of the 16S rRNA gene. Bacterial and archaeal communities were different in the sediments underlying the halocline waters of the three habitats, reflecting the unique chemical settings of each basin. The relative abundance of unique operational taxonomic units (OTUs) was also different between deep--sea control sediments and sediments underlying DHAB haloclines, suggesting adaptation to the steep DHAB chemical gradients. Only a few OTUs were affiliated to known bacterial halophilic and/or aerobic groups. Many OTUs, including some of the dominant ones, were related to aerobic taxa. Archaea were detected only in few halocline samples, with lower OTU richness relative to Bacteria, and were dominated by taxa associated with methane cycling. This study suggests that while metabolically active prokaryotic communities appear to be present in sediments underlying the three DHABs investigated, their diversity and activity are likely to be more reduced in sediments underlying the brines.3

show abstract

Section: Methodssupporting

confidence: 72%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Though the methylamine degradation pathway in the genus Bacillus is poorly characterized, all Gram-positive methylotrophs studied to date use the methylamine oxidase pathway (Chistoserdova et al, 2009). Methylamine SIP sequences from our study clustered with the 16S rRNA gene sequences of both methylotrophic Bacillus strains (Arfman et al, 1992;Anesti et al, 2005) and strains isolated from contaminated soils (Desai et al, 2009;Stobdan et al, unpublished) and marine sediments (Dick et al, 2006;Sass et al, 2008) (Figure 3). This is the first SIP study to identify methylamine-utilizing Bacillus spp.…”

Section: Discussionmentioning

confidence: 80%

Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact

et al. 2010

View full text Add to dashboard Cite

Lonar Lake is a unique saline and alkaline ecosystem formed by meteor impact in the Deccan basalts in India around 52 000 years ago. To investigate the role of methylotrophy in the cycling of carbon in this unusual environment, stable-isotope probing (SIP) was carried out using the onecarbon compounds methane, methanol and methylamine. Denaturing gradient gel electrophoresis fingerprinting analyses performed with heavy 13 C-labelled DNA retrieved from sediment microcosms confirmed the enrichment and labelling of active methylotrophic communities. Clone libraries were constructed using PCR primers targeting 16S rRNA genes and functional genes. Methylomicrobium, Methylophaga and Bacillus spp. were identified as the predominant active methylotrophs in methane, methanol and methylamine SIP microcosms, respectively. Absence of mauA gene amplification in the methylamine SIP heavy fraction also indicated that methylamine metabolism in Lonar Lake sediments may not be mediated by the methylamine dehydrogenase enzyme pathway. Many gene sequences retrieved in this study were not affiliated with extant methanotrophs or methylotrophs. These sequences may represent hitherto uncharacterized novel methylotrophs or heterotrophic organisms that may have been cross-feeding on methylotrophic metabolites or biomass. This study represents an essential first step towards understanding the relevance of methylotrophy in the soda lake sediments of an unusual impact crater structure.

show abstract

“…Ba cil lus spe cies, iden ti fied in this study, were reported to be wide spread in sa line wa ters. B. pumilus and B. licheniformis were iso lated from sea wa ter of dif fer ent ar eas of the Pa cific Ocean (Ivanova et al, 1999), as well as from deep ocean sed i ments (Ettoumi et al, 2009) and deep sea, hypersaline anoxic brine lakes (Sass et al, 2008). Many Ba cillus spe cies, in clud ing B. licheniformis and B. methylotrophicus, were iden ti fied among halophilic and halotolerant iso lates retrieved from the brines of Karak Salt Mines in Pa ki stan.…”

Section: A1 Wetlands 99mentioning

confidence: 99%

Distribution of bacteria in the mineral waters of the Polish Lowlands

Walczak

Deja-Sikora

Kalwasińska

et al. 2016

View full text Add to dashboard Cite

The pa per pres ents the re sults of re search into the dis tri bu tion of mi cro or gan isms in brines with the to tal dis solved sol ids of 40-80 g/dm 3 , found in the Pa leo zoic plat form of the Pol ish Low lands. Wa ter sam ples were col lected from bore holes ex tracting wa ter from the Ju ras sic and Tri as sic aqui fers. The to tal num ber of mi cro or gan isms in the ex am ined wa ter sam ples ranged from 10 to 30 ´ 10 3 cells per 1 cm 3 , while their vi a bil ity ranged from 14 to 58%. The sam ples con tained heterotrophic, chemoautotrophic, aer o bic and an aer o bic bac te ria as well as bac te ria par tic i pat ing in the trans for ma tion of iron and sul phur com pounds. Mo lec u lar iden ti fi ca tion of culturable bac te ria iso lated from wa ter sam ples re vealed that they be long to six genera:

show abstract

Diversity of Bacillus-like organisms isolated from deep-sea hypersaline anoxic sediments

Cited by 63 publications

References 54 publications

Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins

Inter-comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins

Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact

Distribution of bacteria in the mineral waters of the Polish Lowlands

Contact Info

Product

Resources

About