Diversity of Bacteria and Fungi in Aerosols During Screening in a Green Waste Composting Plant

Abstract

This article outlines a comprehensive analysis of the microbial diversity of aerosols produced during screening in a green waste composting plant using both culture and molecular techniques. Bacteria, thermophilic actinomycetes and fungi were quantified in the aerosols. The structure of the microbial community was examined using a fingerprint technique and DNA libraries. The results show: (i) the very high diversity of bacteria and fungi in aerosols produced during the composting screening stage, (ii) the low percentage of cultivability for bacteria in aerosols, (iii) the abundance of Thermoactinomyces spp. and Aspergillus spp. in compost aerosols.

Reliable Procedure for Molecular Analysis of Airborne Microflora in Three Indoor Environments: An Office and Two Different Museum Contexts

“…In fact, it has been applied elsewhere to the diversity of microbial aerosols from biogas [19] and composting sites [20]. In order to assess microbial diversity in air, a single PCR-SSCP amplification was applied to the samples obtained using the different collectors.…”

“…Some molecular fingerprinting techniques have already been applied to analyzing the community structure of bioaerosols in outdoor air [6,18], in biogas [19] and at composting sites [20], but not in enclosed spaces (houses, offices, museums, hospitals, schools, etc.). Initial descriptions of airborne microbes have revealed their broad diversity and the presence of all the domains: Bacteria, Eukarya, and Archaea [1,7,8,21].…”

Bioaerosols from composting facilities—a review

“…In two studies on aerosols collected during the turning of composting piles in the thermophilic phase (Le Goff et al, 2010) and during the screening of matured compost (Bru-Adan et al, 2009), Fimicutes and Actinobacteria were the two dominant bacterial phyla. From sequencing data present in public databases, it appears that Firmicutes , Proteobacteria , and Bacteroidetes are more dominant in compost than are Actinobacteria .…”

Enrichment of microbial communities tolerant to the ionic liquids tetrabutylphosphonium chloride and tributylethylphosphonium diethylphosphate

“…The dominant members in the final enrichment were Bacillaceae (p_Firmicutes) and Acinetobacter (p-Proteobacteria). Members of the family Bacillaceae have well documented cellulolytic activity (Eichorst et al 2013) and have been found in cellulose-rich environments including landfill refuse (Pourcher et al 2001), green waste (Bru-Adan et al 2009) and cattle waste fed biodigesters (Singh and Jain 1986). Acinetobacter belongs to the family Moraxellaceae, a proteobacteria with reported ability to degrade cellulose and hemicellulose of sugarcane (Rinke et al 2011).…”