Diversity of bacteriophages encoding Panton-Valentine leukocidin in temporally and geographically related Staphylococcus aureus

Coombs, Geoffrey W.; Baines, Sarah L.; Howden, Benjamin P; Swenson, Krister M.; O’Brien, Frances

doi:10.1371/journal.pone.0228676

Cited by 17 publications

(20 citation statements)

References 48 publications

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“…It is worth noting, however, that only 13% of the intact prophages carried lukS-PV and lukF-PV. Similar studies of temporally and geographically related S. aureus strains have found a low occurrence of PVL positive strains [51,56].…”

Section: Discussionsupporting

confidence: 64%

Examination of Staphylococcus aureus Prophages Circulating in Egypt

Ene

Miller-Ensminger

et al. 2021

Viruses

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Staphylococcus aureus infections are of growing concern given the increased incidence of antibiotic resistant strains. Egypt, like several other countries, has seen alarming increases in methicillin-resistant S. aureus (MRSA) infections. This species can rapidly acquire genes associated with resistance, as well as virulence factors, through mobile genetic elements, including phages. Recently, we sequenced 56 S. aureus genomes from Alexandria Main University Hospital in Alexandria, Egypt, complementing 17 S. aureus genomes publicly available from other sites in Egypt. In the current study, we found that the majority (73.6%) of these strains contain intact prophages, including Biseptimaviruses, Phietaviruses, and Triaviruses. Further investigation of these prophages revealed evidence of horizontal exchange of the integrase for two of the prophages. These Egyptian S. aureus prophages are predicted to encode numerous virulence factors, including genes associated with immune evasion and toxins, including the Panton–Valentine leukocidin (PVL)-associated genes lukF-PV/lukS-PV. Thus, prophages are likely to be a major contributor to the virulence of S. aureus strains in circulation in Egypt.

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Section: Discussionsupporting

confidence: 64%

Examination of Staphylococcus aureus Prophages Circulating in Egypt

Ene

Miller-Ensminger

et al. 2021

Viruses

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“…ORF63 and ORF64 encoded proteins that were nearly identical to amidase (N-acetylmuramidase), and ORF62 encoded a protein identical to holin. In the lysogeny modules, the predicted proteins of ORF02 and ORF03 were identi ed as integrase and showed highest similarity to that of S. aureus ΦSa2aw_st8 [13]. Finally, the predicted proteins of ORF65 and ORF66 showed similarity to S. aureus Φ7401PVL, with 100% identity [14].…”

Section: Discussionmentioning

confidence: 99%

Phage JS02, a putative temperate phage, a novel biofilm-degrading agent for Staphylococcus aureus

Zhang

Shahin

Soleimani‐Delfan

et al. 2022

Letters in Applied Microbiology

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Background: Staphylococcus aureus is a bio lm-producing organism that is frequently isolated from various environments worldwide. Because of the natural resistance of S. aureus bio lm to antibiotics, bacteriophages are considered as a promising alternative for its removal.Results: The bacteriophage vB_SauS_JS02 was isolated from livestock wastewater and showed activity against multidrug-resistant (MDR) S. aureus. The phage vB_SauS_JS02 was morphologically classi ed as Siphoviridae; it had a broad host range (45 out of 81 strains, 55.6%) and high burst size (52 plaqueforming unit (PFU)/infected cell) and could survive in a pH range of 4 to 11 and a temperature range of 40 ºC to 50 ºC. Bioinformatics analysis showed that the phage genome contained a long double-stranded linear DNA genome of 46,435 base pairs with a G C content of 33.1% and had 66 putative open reading frames (ORFs). The predicted protein products of the ORFs were clustered functionally into ve groups as follows: replication/regulation, DNA packaging, structure/morphogenesis, lysis, and lysogeny. The phage vB_SauS_JS02 was a temperate phage with a higher inhibiting and degrading activity against planktonic cells (~86% reduction) and S. aureus bio lm (∼68% reduction in bio lm formation). Moreover, the removal activity of the phage vB_SauS_JS02 against both planktonic cells and S. aureus bio lms was even better than that of the antibiotic (ceftazidime). Conclusion:In summary, the present study introduced the phage vB_SauS_JS02 as a potential biocontrol agent against bio lm-producing S. aureus.

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“…Genome analysis of the phage revealed 66 predicted ORFs; however, most of the annotated genes (39/66 ORFs) were (N-acetylmuramidase), and ORF62 encoded a protein identical to holin. In the lysogeny modules, the predicted proteins of ORF02 and ORF03 were identi ed as integrase and showed highest similarity to that of S. aureus ΦSa2aw_st8 [13]. Finally, the predicted proteins of ORF65 and ORF66 showed similarity to S. aureus Φ7401PVL, with 100% identity [14].…”

Section: Discussionmentioning

confidence: 99%

Phage JS02, A Putative Temperate Phage, A Novel Biofilm-Degrading Agent for Staphylococcus Aureus

Zhang

Ding

Shahin

et al. 2020

Preprint

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Background: Staphylococcus aureus is a biofilm-producing organism that is frequently isolated from various environments worldwide. Because of the natural resistance of S. aureus biofilm to antibiotics, bacteriophages are considered as a promising alternative for its removal. Results: The bacteriophage vB_SauS_JS02 was isolated from livestock wastewater and showed activity against multidrug-resistant (MDR) S. aureus. The phage vB_SauS_JS02 was morphologically classified as Siphoviridae; it had a broad host range (45 out of 81 strains, 55.6%) and high burst size (52 plaque-forming unit (PFU)/infected cell) and could survive in a pH range of 4 to 11 and a temperature range of 40 ºC to 50 ºC. Bioinformatics analysis showed that the phage genome contained a long double-stranded linear DNA genome of 46,435 base pairs with a G＋C content of 33.1% and had 66 putative open reading frames (ORFs). The predicted protein products of the ORFs were clustered functionally into five groups as follows: replication/regulation, DNA packaging, structure/morphogenesis, lysis, and lysogeny. The phage vB_SauS_JS02 was a temperate phage with a higher inhibiting and degrading activity against planktonic cells (~86% reduction) and S. aureus biofilm (∼68% reduction in biofilm formation). Moreover, the removal activity of the phage vB_SauS_JS02 against both planktonic cells and S. aureus biofilms was even better than that of the antibiotic (ceftazidime). Conclusion: In summary, the present study introduced the phage vB_SauS_JS02 as a potential biocontrol agent against biofilm-producing S. aureus.

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Diversity of bacteriophages encoding Panton-Valentine leukocidin in temporally and geographically related Staphylococcus aureus

Cited by 17 publications

References 48 publications

Examination of Staphylococcus aureus Prophages Circulating in Egypt

Examination of Staphylococcus aureus Prophages Circulating in Egypt

Phage JS02, a putative temperate phage, a novel biofilm-degrading agent for Staphylococcus aureus

Phage JS02, A Putative Temperate Phage, A Novel Biofilm-Degrading Agent for Staphylococcus Aureus

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