Diversity of Botryosphaeriaceae Species Associated with Grapevine Trunk Diseases in the Czech Republic

Abstract

During a study of Botryosphaeriaceae species associated with grapevine trunk diseases in the Czech Republic, a collection of 22 Botryosphaeriaceae-like strains were isolated from four cultivars (Blaufränkisch, Pálava, Pinot Noir, and Welschriesling) in four distinct vineyards. Based on morphology and DNA sequence data (ITS, tub2, and tef), four species were identified: Botryosphaeria dothidea, Diplodia mutila, D. seriata, and Neofusicoccum parvum. These species are reported for the first time from grapevine in the Czech Republic. Relationships between vascular lesions and particular species were highlighted in this study. Diplodia seriata was the most frequently isolated species, present in all four sampled cultivars, while D. mutila was the least frequent, present only in ‘Pálava’. The cultivar Pinot Noir was the most tolerant host for Botryosphaeriaceae fungi.

Characterization of the RNA Mycovirome Associated with Grapevine Fungal Pathogens: Analysis of Mycovirus Distribution and Their Genetic Variability within a Collection of Botryosphaeriaceae Isolates

“…More than 22 species of Botryosphaeriaceae have been recorded on grapevine, with different distributions depending on the country and climate [ 6 , 7 , 8 ]. Among the Botryosphaeriaceae genera and species described in vineyards, Diplodia seriata is the most abundant species, along with Neofusicoccum parvum , Lasiodiplodia theobromae , and Botryosphaeria dothidea [ 4 , 9 ].…”