“…In Togo, cassava occupies an important place in the food production of local populations and is one of the most cultivated crops in all regions of the country in pure culture or in association with other crops. Moreover, according to the results of a survey in cassava production areas in Togo, a total of 168 cultivated cassava varieties were identified in only 40 villages [4].…”
Section: Introductionmentioning
confidence: 99%
“…2. Electrophoretogram showing the results obtained with the SSRY69 primer pair on 18 samplesM = marker; sequence number of positive samples: 1, 2,3,4,5,6,7,8,11,12,13, 16,18; sequence number of negative samples:9,10,14,15,17…”
Cassava (Manihot esculenta Crantz), a plant of the future, is one of the main crops with high genetic potential. Therefore, the knowledge of its genetic variability would be of great importance for its effective use in genetic improvement programs. The objective of this work was to characterize the genetic diversity of cassava cultivars present in the Kara region of Togo. Thus, a total of 91 cassava leaf samples collected in the 7 prefectures of the Kara region were analyzed using 7 pairs of SSR microsatellite primers. Fifty-three alleles were detected with an average of 7.57 alleles per locus. The polymorphic information content ranged from 0.63 to 0.83 with an average of 0.74, indicating a high level of marker polymorphism. Molecular analysis of variance revealed that the majority of variability occurred within individuals and accounted for 99 % of the total variation between individuals. Weak genetic differentiation (Fst=0.011) was also observed between populations. The genetic structuring model based on the Neighbour-Joining algorithm method divided the individuals into 6 groups independently of local names. These results indicate the presence of high genetic resource variability in the studied populations. Therefore, it would be important to implement management strategies to better conserve cassava genetic resources and to facilitate the identification of successful cultivars.
“…In Togo, cassava occupies an important place in the food production of local populations and is one of the most cultivated crops in all regions of the country in pure culture or in association with other crops. Moreover, according to the results of a survey in cassava production areas in Togo, a total of 168 cultivated cassava varieties were identified in only 40 villages [4].…”
Section: Introductionmentioning
confidence: 99%
“…2. Electrophoretogram showing the results obtained with the SSRY69 primer pair on 18 samplesM = marker; sequence number of positive samples: 1, 2,3,4,5,6,7,8,11,12,13, 16,18; sequence number of negative samples:9,10,14,15,17…”
Cassava (Manihot esculenta Crantz), a plant of the future, is one of the main crops with high genetic potential. Therefore, the knowledge of its genetic variability would be of great importance for its effective use in genetic improvement programs. The objective of this work was to characterize the genetic diversity of cassava cultivars present in the Kara region of Togo. Thus, a total of 91 cassava leaf samples collected in the 7 prefectures of the Kara region were analyzed using 7 pairs of SSR microsatellite primers. Fifty-three alleles were detected with an average of 7.57 alleles per locus. The polymorphic information content ranged from 0.63 to 0.83 with an average of 0.74, indicating a high level of marker polymorphism. Molecular analysis of variance revealed that the majority of variability occurred within individuals and accounted for 99 % of the total variation between individuals. Weak genetic differentiation (Fst=0.011) was also observed between populations. The genetic structuring model based on the Neighbour-Joining algorithm method divided the individuals into 6 groups independently of local names. These results indicate the presence of high genetic resource variability in the studied populations. Therefore, it would be important to implement management strategies to better conserve cassava genetic resources and to facilitate the identification of successful cultivars.
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