Diversity of Catechin in Northeast Indian Tea Cultivars

Sabhapondit, Santanu; Karak, Tanmoy; Bhuyan, Lakshi Prasad; Goswami, Bhabesh C.; Hazarika, Manjil

doi:10.1100/2012/485193

Cited by 48 publications

(32 citation statements)

References 23 publications

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“…Catechins are water soluble flavanols that constitute ;25% of the dry weight of green tea, and epigallocatechin gallate (EGCG) accounts for approximately half of the tea catechins (Sabhapondit et al, 2012). Previous work on the interactions of SULTs and dietary chemicals revealed that EGCG is a potent inhibitor of SULT1A1 (K i = 42 nM).…”

Section: Resultsmentioning

confidence: 99%

The Allosteric Binding Sites of Sulfotransferase 1A1

et al. 2014

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Human sulfotransferases (SULTs) comprise a small, 13-member enzyme family that regulates the activities of thousands of compoundsendogenous metabolites, drugs, and other xenobiotics. SULTs transfer the sulfuryl-moiety (-SO 3 ) from a nucleotide donor, PAPS (39-phosphoadenosine 59-phosphosulfate), to the hydroxyls and primary amines of acceptors. SULT1A1, a progenitor of the family, has evolved to sulfonate compounds that are remarkably structurally diverse. SULT1A1, which is found in many tissues, is the predominant SULT in liver, where it is a major component of phase II metabolism. Early work demonstrated that catechins and nonsteroidal antiinflammatory drugs inhibit SULT1A1 and suggested that the inhibition was not competitive versus substrates. Here, the mechanism of inhibition of a single, high affinity representative from each class [epigallocatechin gallate (EGCG) and mefenamic acid] is determined using initial-rate and equilibrium-binding studies. The findings reveal that the inhibitors bind at sites separate from those of substrates, and at saturation turnover of the enzyme is reduced to a nonzero value. Further, the EGCG inhibition patterns suggest a molecular explanation for its isozyme specificity. Remarkably, the inhibitors bind at sites that are separate from one another, and binding at one site does not affect affinity at the other. For the first time, it is clear that SULT1A1 is allosterically regulated, and that it contains at least two, functionally distinct allosteric sites, each of which responds to a different class of compounds.

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Section: Resultsmentioning

confidence: 99%

The Allosteric Binding Sites of Sulfotransferase 1A1

et al. 2014

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“…The variations of EGCG and EGC were the most prominent among the different tea varieties. High EGC content was found to be a characteristic of the Assam variety which was further corroborated through multivariate analysis (Sabhapondit et al 2012). Although accuracy was higher than morphological markers, accumulations of chemicals were subjected to post-transcriptional modification which often restricted their utility as markers (Staub et al 1982).…”

Section: Biochemical Markersmentioning

confidence: 93%

Molecular Markers

Mondal

2014

Breeding and Biotechnology of Tea and Its Wild Species

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“…However, no attempts have been made in systematic characterization of tea germplasm using biochemical parameters of Sri Lankan tea genetic resources. On the contrary, some other tea producing countries such as China, Japan, India and Kenya have used biochemical constituents like total catechins and their fractions, total polyphenols, chlorophylls, carotenoids and caffeine in the fresh leaf as discriminative markers for characterizing their tea germplasms to evaluate diversity and genetic potential warehoused in the germplasm (Magoma et al, 2003;Chen & Zhou, 2005;Lopez et al, 2005;Saravanan et al, 2005;Gulati et al, 2009;Sabhapondit et al, 2012).…”

Section: December 2013mentioning

confidence: 99%

Use of biochemical compounds in tea germplasm characterization and its implications in tea breeding in Sri Lanka

Kottawa-Arachchi¹,

Gunasekare²,

Ranatunga³

et al. 2013

J. Natn. Sci. Foundation Sri Lanka

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Thirty five tea germplasm accessions selected to represent the germpalsm collection of Sri Lanka was used for biochemical characterization based on the biochemical compounds present in the fresh tea leaf. Rate of fermentation, crude fibre content, total polyphenols, total catechins, chlorophyll-a, chlorophyll-b and total carotenoids were analysed. Principal component analysis (PCA) using 7 biochemical parameters and clustering on first three principal components accounted for 87 % of the total variation and delineated the 35 accessions into 4 clusters. Biochemical parameters such as fermentation rate, total polyphenols, total catechins and plant pigments in green leaf were important in explaining the biochemical variation. The selected 35 accessions could also be categorized into 4 groups based on the fermentation rate. Estate selections (PK2, N2, DUN7, S106, DT1, TC9, WT26 and NAY3) and some introductions (TRI777, INTRI6 and VHMOR) recorded the highest total polyphenols and catechin content. Besides, ASM4/10, TRI2016, TRI2025 and TRI2043 showed the highest content of chlorophylls and total carotenoids. The significant variation of the selected biochemical compounds detected in the present study indicated the high genetic diversity of tea germplasm in Sri Lanka. Results suggests that accessions PLLG2, VHMOR, DUN7 and WT26 possess unique characteristics such as rapid fermentation, high amount of polyphenols and catechins, and these could be used as potential parents for future tea breeding programmes after analyzing other complementary traits. The present study provides useful guidelines on the use of fresh leaf biochemical parameters such as the rate of fermentation, total polyphenols, total catechins, chlorophylls and carotenoid content in characterizing the Sri Lankan tea germplasm.

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Diversity of Catechin in Northeast Indian Tea Cultivars

Cited by 48 publications

References 23 publications

The Allosteric Binding Sites of Sulfotransferase 1A1

The Allosteric Binding Sites of Sulfotransferase 1A1

Molecular Markers

Use of biochemical compounds in tea germplasm characterization and its implications in tea breeding in Sri Lanka

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