Diversity of CTX-M-positive Escherichia coli recovered from animals in Canada

Whole Genome Sequencing Differentiates Presumptive Extended Spectrum Beta-Lactamase Producing Escherichia coli along Segments of the One Health Continuum

“…It may be deduced that ESBL-EC in human-sourced samples were levels of magnitude higher, possibly selected on the basis of clinical illness and/or resistant infections. Variants of bla CTX-M-1,-14,-27 were only associated with ESBL isolates from humans, a finding which is compatible with other reports of bla CTX-M-14,-15,-27 association with the global spread of bla CTX-M in human clinical isolates [1,75,76]. Although bla CTX-M-15 was more frequently detected in human-than cattle-related isolates, their occurrence in both cattle-and human-sources may possibly imply transmission between humans and animals [75,77] as ESBL-EC can be zoonotic [78].…”

“…Variants of bla CTX-M-1,-14,-27 were only associated with ESBL isolates from humans, a finding which is compatible with other reports of bla CTX-M-14,-15,-27 association with the global spread of bla CTX-M in human clinical isolates [1,75,76]. Although bla CTX-M-15 was more frequently detected in human-than cattle-related isolates, their occurrence in both cattle-and human-sources may possibly imply transmission between humans and animals [75,77] as ESBL-EC can be zoonotic [78]. In this study, bla SHV genes were rather rare in contrast to other Canadian investigations where bla SHV2 variants have been frequently reported [75,79].…”

“…Interestingly, different STs prevailed in different sources, even between CBasins and SStreams. Similar to other reports, ST244 were dominant [75] in cattle-sources, while hyperendemic ExPEC ST131 (implicated in bloodstream and urinary tract infection) [76] and, to a lesser degree, STs 38 and 648 were predominant in human-sources. E. coli ST648 exhibiting an ESBL phenotype have been reported globally in human patients [7,75].…”

“…Although bla CTX-M-15 was more frequently detected in human-than cattle-related isolates, their occurrence in both cattle-and human-sources may possibly imply transmission between humans and animals [75,77] as ESBL-EC can be zoonotic [78]. In this study, bla SHV genes were rather rare in contrast to other Canadian investigations where bla SHV2 variants have been frequently reported [75,79]. An interesting finding of the current work is that both bla SHV-11 and 12 were detected in a single isolate from CFeces.…”

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

“…Faecal (Velasova et al, 2019) Infection (Hunter et al, 2010) ND (Stokes, 2014) Czech Republic Faecal (Dolejska et al, 2011b) Sick (Dolejska et al, 2013) R eunion ND (Gay et al, 2018) Slovakia Faecal (Kme t and Buj n akov a, 2018) Sweden Faecal (Duse et al, 2015) Switzerland Faecal (Endimiani et al, 2012;Geser et al, 2012a;Zurfluh et al, 2015) Turkey Faecal (AslantaŞ et al, 2017;Pehlivanoglu et al, 2016) North America Canada ND (Cormier et al, 2019) USA Faecal (Mir et al, 2016;Mollenkopf et al, 2012;Wittum et al, 2010) Asia China Mastitis (Ali et al, 2016(Ali et al, , 2017 South Korea Mastitis (Tark et al, 2017) Indonesia Faecal (Sudarwanto et al, 2016) Japan Mastitis (Ohnishi et al, 2013b) Klebsiella pneumoniaeEurope Italy Mastitis (Locatelli et al, 2010) Klebsiella ozaenae Italy Faecal (Stefani et al, 2014) Salmonella enterica Germany ND (Rodríguez et al, 2009) CTX-M-1/61 E. coli Europe Germany Faecal (Dahms et al, 2015) CTX-M-2 E. coli South America Brazil Faecal (Palmeira et al, 2018) Europe Germany Mastitis (Eisenberger et al, 2017;Freitag et al, 2017;Michael et al, 2017) Sick (Michael et al, 2017) Netherlands Faecal (Ceccarelli et al, 2019;Heuvelink et al, 2019) North America Canada Faecal (Cormier et al, 2016) Asia Japan Faecal (Shiraki et al, 2004) Diarrheic (Ohnishi et al, 2013a) Infection (Asai et al, 2011) K. pneumoniae Japan Mastitis (Ohnishi et...…”