Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.

Jiang, Shumei; Sun, Wei; Chen, Minjie; Dai, Shikun; Zhang, Long; Liu, Yonghong; Lee, Kyung Jin; Li, Xiang

doi:10.1007/s10482-007-9169-z

Cited by 102 publications

(78 citation statements)

References 57 publications

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“…This group has one cultured representative, strain 1M83 (DQ994722), isolated from the marine sponge Haliclona sp. on streptomycete medium by Jiang et al (11). This bacterium is the first known sponge isolate from the Acidobacteria, and examination of this organism may give insight into the cultivation of other Acidobacteria from sponges.…”

Section: Discussionmentioning

confidence: 98%

Sponge-Associated Bacteria Are Strictly Maintained in Two Closely Related but Geographically Distant Sponge Hosts

Montalvo

Hill

2011

Appl Environ Microbiol

110

103

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The giant barrel sponges Xestospongia muta and Xestospongia testudinaria are ubiquitous in tropical reefs of the Atlantic and Pacific Oceans, respectively. They are key species in their respective environments and are hosts to diverse assemblages of bacteria. These two closely related sponges from different oceans provide a unique opportunity to examine the evolution of sponge-associated bacterial communities. Mitochondrial cytochrome oxidase subunit I gene sequences from X. muta and X. testudinaria showed little divergence between the two species. A detailed analysis of the bacterial communities associated with these sponges, comprising over 900 full-length 16S rRNA gene sequences, revealed remarkable similarity in the bacterial communities of the two species. Both sponge-associated communities include sequences found only in the two Xestospongia species, as well as sequences found also in other sponge species and are dominated by three bacterial groups, Chloroflexi, Acidobacteria, and Actinobacteria. While these groups consistently dominate the bacterial communities revealed by 16S rRNA gene-based analysis of sponge-associated bacteria, the depth of sequencing undertaken in this study revealed clades of bacteria specifically associated with each of the two Xestospongia species, and also with the genus Xestospongia, that have not been found associated with other sponge species or other ecosystems. This study, comparing the bacterial communities associated with closely related but geographically distant sponge hosts, gives new insight into the intimate relationships between marine sponges and some of their bacterial symbionts.Marine sponges (phylum Porifera) are ancient metazoans with a fossil record dating back over 600 million years, but the duration of the sponge-bacterium association is less well documented. Sponges actively pump seawater through pores and channels to obtain food consisting mainly of microbes filtered from the surrounding seawater, but they have no true organs or circulatory, nervous, or digestive systems. Bacterial symbionts may play roles in digestion, waste removal, and chemical defense for the sponge host (38). We use the terms "symbiont" and "symbiosis" in the same sense as Taylor et al. (38) and consistent with the original definition of de Bary to refer to two or more organisms consistently found living together for a long period without any implication that the organisms benefit or harm each other.Many sponges contain chemical compounds with bioactive properties, several of which are promising as pharmaceutical leads, and sponge-associated microorganisms are of interest due to their potential role in producing some of these compounds (10). Studies of the bacteria associated with marine sponges have revealed novel bacterial groups, such as the candidate phylum "Poribacteria" (6, 16), as well as ubiquitous symbionts found in many different sponges, including the NW001-like Alphaproteobacteria (4, 46). Sponge-microbe associations provide a model for studying ancient, complex symbioses ...

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Section: Discussionmentioning

confidence: 98%

Sponge-Associated Bacteria Are Strictly Maintained in Two Closely Related but Geographically Distant Sponge Hosts

Montalvo

Hill

2011

Appl Environ Microbiol

110

103

View full text Add to dashboard Cite

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“…In classical isolation techniques, the sponge samples were directly stamped on isolation media or in the dilution series method, sponge samples are crashed with a mortar, placed into sea water and after sedimentation, plated in different dilutions on specific sponge isolation agar (Mantalvo et al 2005;Jiang et al 2007). However, there are also some modifications of this method.…”

Section: Isolation Of Marine Actinobacteriamentioning

confidence: 99%

Actinobacteria and Myxobacteria—Two of the Most Important Bacterial Resources for Novel Antibiotics

Landwehr

Wolf

Wink

2016

Current Topics in Microbiology and Immunology

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“…The 16S rRNA gene was amplified using primers 27F (5'-AGA GTT TGA TCC TGG CTC AG-3 ') and1492 R (5'-GTT TAC CTT GTT ACG ACT T-3 ') (Jiang et al, 2007). Amplification of the 16S rRNA gene was performed in T100 thermal cycler (Bio-Rad) in a total volume of 50 ml as a described above.…”

Section: Pcr Amplifi Cation and Sequencing Of 16s Rrna Genesmentioning

confidence: 99%

“…Amplification of the 16S rRNA gene was performed in T100 thermal cycler (Bio-Rad) in a total volume of 50 ml as a described above. PCR program used to follow Jiang et al (2007) with some modifi cations, the PCR program included initial denaturation at 97°C for 3 min, denaturation at 95°C for 1 min, then followed by annealing at 60°C for 1 min, extension at 72°C for 3 min the whole process lasted about 30 cycles, the last cycle followed by a final extension at 72° C for 10 min and cooling stage at 4°C. The PCR product was visualized by 1% agarose electrophoresis.…”

Section: Pcr Amplifi Cation and Sequencing Of 16s Rrna Genesmentioning

confidence: 99%

“…Enzyme HaeIII restriction endonucleases are enzymes which have a tetra metric site restriction to the four bases, this enzyme cut the DNA sequences Other restriction enzymes can cut the NRPS gene and other genes in actinomycetes such as HinfI can cut NRPS genes, PKS I and II produced a diverse banding pattern (AyusoSacido et al, 2005), while the enzyme MspI and TaqI can cut NRPS gene and 16S rRNA in actinomycetes (Jiang et al, 2007). Based on the NRPS gene cutting pattern of 14 isolates of marine actinomycetes, restriction analysis with restriction endonuclease HaeIII of NRPS genes can be used to analyzed the diversity of NRPS gene sequence among 14 actinomycetes isolates quickly.…”

Section: Diversity Of Nonribosomal Peptide Synthetase Genesmentioning

confidence: 99%

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Diversity of Nonribosomal Peptide Synthetase Genes in the AnticancerProducing Actinomycetes Isolated from Marine Sediment in Indonesia

et al. 2016

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Marine actinomycetes is a group of bacteria that is highly potential in producing novel bioactive compound. It has unique characteristics and is different from other terrestrial ones. Extreme environmental condition is suspected to lead marine actinomycetes produce different types of bioactive compound found previously. The aim of this study was to explore the presence and diversity of NRPS genes in 14 anticancer-producing actinomycetes isolated from marine sediment in Indonesia. PCR amplifi cation and restriction fragment analysis of NRPS genes with HaeIII from 14 marine actinomycetes were done to assess the diversity of NRPS genes. Genome mining of one species of marine actinomycetes (strain GMY01) also was employed towards this goal. The result showed that NRPS gene sequence diversity in 14 marine actinomycetes could be divided into 4 groups based on NRPS gene restriction patterns. Analysis of 16S rRNA gene sequences of representatives from each group showed that all isolates belong to genus of Streptomyces. Genome mining result showed that strain GMY01 harboring 10 different NRPS gene clusters that encode secondary metabolites, as pure NRPS or hybrid between NRPS and other compounds. These results indicated that marine actinomycetes having a high potential to be developed as source of anticancer drugs development. Keyword IntroductionWide range of bioactive compounds metabolites were isolated and identified from soil actinomycetes. Recently, the rate of new metabolites discovery from terrestrial

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Diversity of culturable actinobacteria isolated from marine sponge Haliclona sp.

Cited by 102 publications

References 57 publications

Sponge-Associated Bacteria Are Strictly Maintained in Two Closely Related but Geographically Distant Sponge Hosts

Sponge-Associated Bacteria Are Strictly Maintained in Two Closely Related but Geographically Distant Sponge Hosts

Actinobacteria and Myxobacteria—Two of the Most Important Bacterial Resources for Novel Antibiotics

Diversity of Nonribosomal Peptide Synthetase Genes in the AnticancerProducing Actinomycetes Isolated from Marine Sediment in Indonesia

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