Diversity of Culturable Bacteria Isolated from Highland Barley Cultivation Soil in Qamdo, Tibet Autonomous Region

Pan, Hu; Zhou, Jie; Dawa, Zhuoma; Dai, Yanna; Zhang, Yifan; Yang, Hui; Wang, Chong; Liu, Huhu; Zhou, Hui; Lu, Xiangyang; Tian, Yun

doi:10.33073/pjm-2021-008

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…In the current study, the positive rate of C. perfringens from yak was lower than that of animals from other regions. The reason may be the long been living on the Tibetan plateau with high altitude, low temperature, low humidity, strong UV light, few airborne particulates and bacterial vectors, that prohibit bacteria from reproduction and transmission ( 29 ). This is in line with the microbial diversity and physicochemical characteristics of soil at high altitudes, where gram negative bacteria proliferate more as compare to gram positive bacteria ( 30 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility and multilocus sequence typing of Clostridium perfringens isolated from yaks in Qinghai-Tibet plateau, China

Luo

Gong

et al. 2022

Front. Vet. Sci.

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Clostridium perfringens (C. perfringens) is an opportunistic pathogen that cause necrotic enteritis, food poisoning and even death in animals. In this study, we explored the prevalence, antibiotic resistance and genetic diversity of Clostridium perfringens isolated from yak in the Qinghai-Tibet plateau, China. A total of 744 yak fecal samples were collected and assessed for toxin genes, antimicrobial susceptibility and multilocus sequence typing (MLST). Results indicated that 144 out of 744 (19.35%) yak fecal samples were tested to be positive for C. perfringens, 75% (n = 108, 108/144) were C. perfringens type A, 17.36% (n = 25, 25/144) were C. perfringens type C, 2.78% (n = 4, 4/144) were C. perfringens type D, and 4.86% (n = 7, 7/144) were C. perfringens type F. In addition, 2.78% (n = 4, 4/144) of the isolates were positive for cpb2 toxin gene. Antimicrobial susceptibility testing revealed that 98.61% (142/144) of the isolates showed multiple-antibiotic resistance. According to MLST and phylogenetic tree, 144 yak-derived C. perfringens isolates had an average of 12.95 alleles and could be divided into 89 sequence types (STs) and clustered in 11 clonal complexes (CCs). The most of isolates belong to type A with a considerable genetic diversity, having Simpson index up to 0.9754. MLST and phylogenetic analysis showed that the isolates under the same clade came from multiple regions. Cross-transmission among isolates and interconnectedness were observed in the genetic evolution. According to the study, the most of the isolates exhibited broad-spectrum antibacterial resistance, diverse alleles, and multiple lethal toxin genes of C. perfringens.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility and multilocus sequence typing of Clostridium perfringens isolated from yaks in Qinghai-Tibet plateau, China

Luo

Gong

et al. 2022

Front. Vet. Sci.

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“…Amplification of 16S rRNA gene was performed under the following conditions: 95°C for 10 min, followed by 94°C for 45 s, 56°C for 45 s, and 72°C for 90 s for 30 cycles with a final 10 min extension at 72°C, the PCR products were detected by agarose gel electrophoresis and then sent to GENEWIZ.lnc for sequencing. Primers used for amplification and sequencing of 16S rRNA was described by Pan et al (2021) . 16S rRNA gene was aligned using EzBioCloud.…”

Section: Methodsmentioning

confidence: 99%

Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet

Pan

Zhu

et al. 2023

Front. Microbiol.

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A bacterial strain A1-3 with iprodione-degrading capabilities was isolated from the soil for vegetable growing under greenhouses at Lhasa, Tibet. Based on phenotypic, phylogenetic, and genotypic data, strain A1-3 was considered to represent a novel species of genus Azospirillum. It was able to use iprodione as the sole source of carbon and energy for growth, 27.96 mg/L (50.80%) iprodione was reduced within 108 h at 25°C. During the degradation of iprodione by Azospirillum sp. A1-3, iprodione was firstly degraded to N-(3,5-dichlorophenyl)-2,4-dioxoimidazolidine, and then to (3,5-dichlorophenylurea) acetic acid. However, (3,5-dichlorophenylurea) acetic acid cannot be degraded to 3,5-dichloroaniline by Azospirillum sp. A1-3. A ipaH gene which has a highly similarity (98.72–99.92%) with other previously reported ipaH genes, was presented in Azospirillum sp. A1-3. Azospirillum novel strain with the ability of iprodione degradation associated with nitrogen fixation has never been reported to date, and Azospirillum sp. A1-3 might be a promising candidate for application in the bioremediation of iprodione-contaminated environments.

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“…Ampli cation of 16S rRNA gene was performed under the following conditions: 95℃ for 10 min, followed by 94℃ for 45 s, 56℃ for 45 s, and 72℃ for 90 s for 30 cycles with a nal 10 min extension at 72℃, the PCR products were detected by agarose gel electrophoresis and then sent to GENEWIZ.lnc for sequencing. Primers used for ampli cation and sequencing of 16S rRNA was as described by Pan et al (2021). 16S rRNA gene was aligned in EzBioCloud (https://eztaxon-e.ezbiocloud.net/).…”

Section: Phenotypic Characterization and 16s Rrna Gene Analysismentioning

confidence: 99%

Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet

Pan

Zhu

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

A bacterial strain A1-3 with iprodione-degrading capabilities was isolated from the soil for vegetable growing in greenhouses at Lhasa, Tibet. Based on phenotypic, phylogenetic, and genotypic data, strain A1-3 was considered to represent a novel species of genus Azospirillum. It was able to use iprodione as the sole source of carbon and energy for growth, 27.96 mg/L iprodione was reduceded within 108 h at 25℃. During the degradation of iprodione by Azospirillum sp. A1-3, iprodione was firstly degraded to N-(3,5-dichlorophenyl)-2,4-dioxoimidazolidine, and then to (3,5-dichlorophenylurea) acetic acid. However, (3,5-dichlorophenylurea) acetic acid cannot be degraded to 3,5-dichloroaniline by Azospirillum sp. A1-3. Azospirillum novel strains with the ability of iprodione degradation associated with nitrogen fixation has never been reported to date, and Azospirillum sp. A1-3 might be a promising candidate for application in the bioremediation of iprodione-contaminated environments.

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Diversity of Culturable Bacteria Isolated from Highland Barley Cultivation Soil in Qamdo, Tibet Autonomous Region

Cited by 8 publications

References 41 publications

Antimicrobial susceptibility and multilocus sequence typing of Clostridium perfringens isolated from yaks in Qinghai-Tibet plateau, China

Antimicrobial susceptibility and multilocus sequence typing of Clostridium perfringens isolated from yaks in Qinghai-Tibet plateau, China

Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet

Degradation of iprodione by a novel strain Azospirillum sp. A1-3 isolated from Tibet

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