Diversity of detoxification pathways of ingested ecdysteroids among phytophagous insects

Rharrabe, Kacem; Alla, Salah; Maria, Annick; Sayah, Fouad; Lafont, René

doi:10.1002/arch.20191

Cited by 34 publications

(23 citation statements)

References 42 publications

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“…Inactivation involves the conversion of active ecdysteroids to more polar metabolites and/or formation of conjugates. In decapods, the two major organs responsible for removing ecdysteroids from the hemolymph are the antennal gland and hepatopancreas [72]. In general, the antennal gland and peripheral tissues are responsible for the conversion of ecdysteroids to inactive polar metabolites, which are then excreted in the urine.…”

Section: Resultsmentioning

confidence: 99%

Insights into Hepatopancreatic Functions for Nutrition Metabolism and Ovarian Development in the Crab Portunus trituberculatus: Gene Discovery in the Comparative Transcriptome of Different Hepatopancreas Stages

et al. 2014

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The crustacean hepatopancreas has different functions including absorption, storage of nutrients and vitellogenesis during growth, and ovarian development. However, genetic information on the biological functions of the crustacean hepatopancreas during such processes is limited. The swimming crab, Portunus trituberculatus, is a commercially important species for both aquaculture and fisheries in the Asia-Pacific region. This study compared the transcriptome in the hepatopancreas of female P. trituberculatus during the growth and ovarian maturation stages by 454 high-throughput pyrosequencing and bioinformatics. The goal was to discover genes in the hepatopancreas involved in food digestion, nutrition metabolism and ovarian development, and to identify patterns of gene expression during growth and ovarian maturation. Our transcriptome produced 303,450 reads with an average length of 351 bp, and the high quality reads were assembled into 21,635 contigs and 31,844 singlets. Based on BLASTP searches of the deduced protein sequences, there were 7,762 contigs and 4,098 singlets with functional annotation. Further analysis revealed 33,427 unigenes with ORFs, including 17,388 contigs and 16,039 singlets in the hepatopancreas, while only 7,954 unigenes (5,691 contigs and 2,263 singlets) with the predicted protein sequences were annotated with biological functions. The deduced protein sequences were assigned to 3,734 GO terms, 25 COG categories and 294 specific pathways. Furthermore, there were 14, 534, and 22 identified unigenes involved in food digestion, nutrition metabolism and ovarian development, respectively. 212 differentially expressed genes (DEGs) were found between the growth and endogenous stage of the hepatopancreas, while there were 382 DEGs between the endogenous and exogenous stage hepatopancreas. Our results not only enhance the understanding of crustacean hepatopancreatic functions during growth and ovarian development, but also represent a basis for further research on new genes and functional genomics of P. trituberculatus or closely related species.

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Section: Resultsmentioning

confidence: 99%

Insights into Hepatopancreatic Functions for Nutrition Metabolism and Ovarian Development in the Crab Portunus trituberculatus: Gene Discovery in the Comparative Transcriptome of Different Hepatopancreas Stages

et al. 2014

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“…PEs are generally toxic to insects because they interfere with ecdysone signaling (Dinan 2001). Although ECB larvae can detoxify PEs by producing fatty acyl ester conjugates (Rharrabe et al 2007), we observed that larvae avoid feeding on a diet that contains PEs. Since ECB larvae and adults avoid PEs, we wondered if ECB larvae would be deterred by odorants emitted by spinach.…”

Section: Introductionmentioning

confidence: 91%

Pulsed Odors from Maize or Spinach Elicit Orientation in European Corn Borer Neonate Larvae

Piesik¹,

Rochat²,

Pers³

et al. 2009

J Chem Ecol

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Lepidoptera larvae are capable of orienting towards or away from plants by using odors as cues but whether this attraction is innate or secondarily acquired remains unknown. We tested the hypothesis that European corn borer (ECB) neonate larvae express an innate attraction towards odors released from maize, and avoidance towards odors from spinach. Neonate larvae were placed on a locomotion compensator within a constant stream of humidified air that was loaded intermittently with airborne odors drawn from potted plants. The odor stream was delivered continuously or pulsed (1 to 10 sec pulses) at 40 ml/min. ECB larvae oriented toward maize odors pulsed at 2 to 6 sec but walked away from maize odors delivered at lower frequencies (9 and 10 sec pulses or to continuous ones). They consistently walked away from spinach odors, irrespective of the pulsing regime except at 1 sec pulses that did not elicit orientation. We further explored odor intensity on orientation towards maize odors by adjusting the odor stream intensity. At higher intensity (60 ml/min), the direction reversal started at the 6 sec half period, while at lower intensity (20 ml/min), it showed up only for the continuous stimulus. ECB larvae exhibit a striking ability to lock on to a direction, which they maintained despite gaps of up to 10 sec in the odor stream. Our results demonstrate that ECB neonate larvae express innate orientation preferences towards natural odors from plants. These reactions correlate well with the biological value of these plants for ECB: maize generally is accepted by ECB larvae and adults, while spinach represents a poor host because it produces (non-volatile) phytoecdysteroids that are toxic and deterrent.

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“…Thus, the two major organs responsible for removing ecdysteroids from the hemolymph are the antennal gland and hepatopancreas (midgut gland) in decapods. The diversity of inactivation pathways in crustaceans is reminiscent to those used by insects to detoxify ecdysteroids ingested from plants (see [98] for review). The primary site for excretion of ecdysteroids is the antennal gland.…”

Section: Ecdysteroid Metabolism and Excretionmentioning

confidence: 99%

Ecdysteroid metabolism in crustaceans

Mykles

2011

The Journal of Steroid Biochemistry and Molecular Biology

178

121

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Diversity of detoxification pathways of ingested ecdysteroids among phytophagous insects

Cited by 34 publications

References 42 publications

Insights into Hepatopancreatic Functions for Nutrition Metabolism and Ovarian Development in the Crab Portunus trituberculatus: Gene Discovery in the Comparative Transcriptome of Different Hepatopancreas Stages

Insights into Hepatopancreatic Functions for Nutrition Metabolism and Ovarian Development in the Crab Portunus trituberculatus: Gene Discovery in the Comparative Transcriptome of Different Hepatopancreas Stages

Pulsed Odors from Maize or Spinach Elicit Orientation in European Corn Borer Neonate Larvae

Ecdysteroid metabolism in crustaceans

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