Diversity of DNA Sequences Among Restriction Endonucleases Producing Selenomonas ruminantium Isolates Detected by Enterobacterial Repetitive Intergenic Consensus Based Polymerase Chain Reaction (ERIC-PCR)

Evaluation of the Biofilm‐Forming Ability and Genetic Typing for Clinical Isolates of Pseudomonas aeruginosa by Enterobacterial Repetitive Intergenic Consensus‐Based PCR

“…Because the ERIC sequences are highly conserved within the eubacterial kingdom (19), they have been used as the PCR primers to characterize many bacterial strains (19,22). The results of the ERIC-based genotyping of P. aeruginosa revealed that 48 clinical isolates were divided into five groups with distinguishable ERIC-PCR patterns.…”

“…Various methods, such as pulsed-field gel electrophoresis, arbitrarily primed polymerase chain reaction, and ribotyping are currently available for genotyping of P. aeruginosa (1,7,15). On the other hand, the enterobacterial repetitive intergenic consensus (ERIC) sequences are known to be dispersed throughout the prokaryotic genome (19,22), and polymerase chain reaction (PCR) studies of eubacteria have revealed that inter-ERIC distances and patterns are highly specific to the individual group of the same bacterial species (22). In P. aeruginosa clinical isolates, correlation of the genetic group determined by ERIC-based PCR (ERIC-PCR) with the ability to form a biofilm has not been reported.…”

“…Serotyping has traditionally been used for bacterial grouping, but it cannot be applied to a mucoid bacterium such as P. aeruginosa. The molecular genotyping by ERIC-PCR is much faster and more cost-effective; furthermore, it possesses a higher discriminatory ability than other typing techniques (19,21,22). Herein, P. aeruginosa isolates, which may possess extensive genomic diversity (10,20), were clearly genotyped by ERIC-PCR.…”