Diversity of Endophytic Fungi Isolated from Korean Ginseng Leaves

Eo, Ju-Kyeong; Choi, Minseok; Eom, Ahn-Heum

doi:10.5941/myco.2014.42.2.147

Cited by 24 publications

(15 citation statements)

References 20 publications

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“…It is known that an endophyte in one plant may act as a pathogen in another plant, depending on the balance between the pathogenicity and endophytism of the microorganism in different hosts. The fungi isolated during this study have previously been isolated as endophytes from a wide range of different host plants such as Pinus tabulaeformis ( 31 ), Avicennia marina ( 32 ), ginseng leaves ( 33 ), Thymus decussatus ( 34 ), and C. procera ( 13 ). Endophytic fungi were tested for antifungal activity using a dual culture method.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Analysis of Antimicrobial Activity of Endophytic Fungi From Medicinal Plants in Saudi Arabia

Gashgari

Gherbawy

Ameen

et al. 2016

Jundishapur J Microbiol

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Background:Endophytic fungi, which have been reported in numerous plant species, are important components of the forest community and contribute significantly to the diversity of natural ecosystems.Objectives:The current study aimed to evaluate and characterize, at the molecular level, the diversity and antimicrobial activities of endophytic fungi from medicinal plants in Saudi Arabia.Materials and Methods:Fungi growing on plant segments were isolated and identified based on morphological and molecular characteristics. The isolates were grouped into 35 distinct operational taxonomic units, based on the sequence of the internal transcribed spacer regions in the rRNA gene. The colonization frequency and the dominant fungi percentage of these endophytic fungi were calculated. A dual culture technique was adopted to investigate the antifungal activity of these endophytes.Results:Tamarix nilotica showed the highest endophytic diversity with a relative frequency of 27.27%, followed by Cressa cretica with a relative frequency of 19.27%. The most frequently isolated species was Penicillium chrysogenum with an overall colonization rate of 98.57%. Seven out of 35 endophytic fungi exhibited strong antifungal activity to all plant fungal pathogens tested. P. chrysogenum, Fusarium oxysporum, and F. nygamai exhibited the highest inhibition against the human pathogenic bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Aspergillus sydowii, P. chrysogenum, and Eupenicillium crustaceum showed strong antimicrobial activity against Enterococcus faecalis.Conclusions:The antimicrobial activity of these endophytic microorganisms could be exploited in biotechnology, medicine, and agriculture.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Analysis of Antimicrobial Activity of Endophytic Fungi From Medicinal Plants in Saudi Arabia

Gashgari

Gherbawy

Ameen

et al. 2016

Jundishapur J Microbiol

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“…The reported pathogens include Alternaria panax , Alternaria tenuissima , Cylindrocarpon destructans , Cylindrocarpon didynum , Fusarium oxysporum , Fusarium solani , Phytophthora cactorum , Phoma herbarum , and Rhizoctonia solani [22] . Fungal pathogens, endophytes, and rhizosphere microbes of Panax ginseng and Panax quinquefolius have been reported [23] , [24] , [25] , [26] , [27] . Investigation on fungi associated with P. notoginseng has been focused on pathogens, and few reports were about the diversity of fungal community in the rhizosphere soil of P. notoginseng.…”

Section: Introductionmentioning

confidence: 99%

Rhizospheric fungi of Panax notoginseng: diversity and antagonism to host phytopathogens

Miao

Qiao

et al. 2016

Journal of Ginseng Research

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BackgroundRhizospheric fungi play an essential role in the plant–soil ecosystem, affecting plant growth and health. In this study, we evaluated the fungal diversity in the rhizosphere soil of 2-yr-old healthy Panax notoginseng cultivated in Wenshan, China.MethodsCulture-independent Illumina MiSeq and culture-dependent techniques, combining molecular and morphological characteristics, were used to analyze the rhizospheric fungal diversity. A diffusion test was used to challenge the phytopathogens of P. notoginseng.ResultsA total of 16,130 paired-end reads of the nuclear ribosomal internal transcribed spacer 2 were generated and clustered into 860 operational taxonomic units at 97% sequence similarity. All the operational taxonomic units were assigned to five phyla and 79 genera. Zygomycota (46.2%) and Ascomycota (37.8%) were the dominant taxa; Mortierella and unclassified Mortierellales accounted for a large proportion (44.9%) at genus level. The relative abundance of Fusarium and Phoma sequences was high, accounting for 12.9% and 5.5%, respectively. In total, 113 fungal isolates were isolated from rhizosphere soil. They were assigned to five classes, eight orders (except for an Incertae sedis), 26 genera, and 43 species based on morphological characteristics and phylogenetic analysis of the internal transcribed spacer. Fusarium was the most isolated genus with six species (24 isolates, 21.2%). The abundance of Phoma was also relatively high (8.0%). Thirteen isolates displayed antimicrobial activity against at least one test fungus.ConclusionOur results suggest that diverse fungi including potential pathogenic ones exist in the rhizosphere soil of 2-yr-old P. notoginseng and that antagonistic isolates may be useful for biological control of pathogens.

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“…The plant is thought to provide nutrients to the endophytic fungi, while the microbe may produce factors that protect the host plant from attack by animals, insects or other microbes 12 . It has been proven that endophytic fungi are a source of providing secondary metabolites diverse in structure and biologic activity 13,14 . Thus, the endophytic fungi in C. acuminata plant are expected to be a potential source for CPT or new natural bioactive agents.…”

Section: Introductionmentioning

confidence: 99%

Characterization and antitumor activity of camptothecin from endophytic fungus <i>Fusarium solani</i> isolated from <i>Camptotheca acuminate</i>

Ran

Zhang

et al. 2017

Afr H. Sci.

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Background: Camptothecin (CPT) is a potent drug against cancers, originally from plants. The endophytic fungi could produce the secondary metabolite same as the host and is used as medicine. Objectives:The aim of this paper was to investigate an endophytic fungal CPT with anti-neoplastic activity. Methods: Endophytic fungi were isolated from Camptotheca acuminata in China. CPT from strain S-019 was characterized by TLC, HPLC and EI-MS analysis. Anti-tumor activity of fungal CPT was detected by MTT and fluorescent dye methods using Vero and PC-3 cells. Results: A total of 94 endophytic fungi strains were isolated from tissues of C. acuminata and 16 fungi strains displayed cytotoxic activity on Vero or PC3 cells. Of which, the fungal strain S-019, classified as Fusarium solani, displayed impressive cytotoxic activity on cancer cells and was found to produce CPT by analysis of TLC, HPLC and EI-MS methods. Bioassay studies confirmed that the fungi CPT had potent cytotoxicity on Vero cells and induced apoptosis of Vero cells. Conclusion:The endophytic fungi from camptotheca trees are a reliable source for natural anticancer compounds. The endophytic fungi could produce CPT same as plant. The fungal CPT exhibited effective activity at inhibiting cell growth and inducing apoptosis on Vero cells.

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Diversity of Endophytic Fungi Isolated from Korean Ginseng Leaves

Cited by 24 publications

References 20 publications

Molecular Characterization and Analysis of Antimicrobial Activity of Endophytic Fungi From Medicinal Plants in Saudi Arabia

Molecular Characterization and Analysis of Antimicrobial Activity of Endophytic Fungi From Medicinal Plants in Saudi Arabia

Rhizospheric fungi of Panax notoginseng: diversity and antagonism to host phytopathogens

Characterization and antitumor activity of camptothecin from endophytic fungus <i>Fusarium solani</i> isolated from <i>Camptotheca acuminate</i>

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