DIVERSITY OF EXTRACELLULAR ENZYMES PRODUCED BY ENDOPHYTIC FUNGUS ORIGINATED FROM Centella asiatica (L.) Urban

Susilowati, Dwi Ningsih; Setiyani, Alfi Dwi; Radiastuti, Nani; Sofiana, Indah; Suryadi, Yadi

doi:10.21082/jlittri.v26n2.2020.78-91

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“…Previous researches have identified several bioactivities, such as extracellular enzymes (asparaginase, amylase, cellulase, pectin, protease, glucanase, laccase) producer, antimicrobial, and antioxidant activity produced by endophytic fungi from Asiatic pennyworth (Susilowati et al 2020). It has been found that the endophytes produce a significant amount of antioxidants, which prevent oxidative damage to cellular components.…”

Section: Introductionmentioning

confidence: 99%

Potential of Endophytic Fungi Deriving From Asiatic Pennyworth to Produce Antioxidants

Susilowati¹,

Radiastuti

Nurhasni

et al. 2021

Indones. J. Agric. Sci.

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Asiatic pennyworth is a medicinal plant that contains triterpenoids, saponin, flavonoids, and tannins which possess antioxidants. Endophytic fungi from the plant could produce a similar compound; therefore, antioxidants could be made in the laboratory if the fungi are isolated. This study aimed to evaluate the potential of endophytic fungi isolated from Asiatic pennyworth to produce antioxidants. The study used 34 endophytic fungal isolates from Asiatic pennyworth accessions of Malaysia (17 isolates) and Bengkulu, Indonesia (17 isolates) collected by the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. The fungi were propagated in a potato dextrose broth medium, then mycelia mats and filtrates were separated and then freeze-dried. The antioxidant activities were first tested with 1,1-diphenyl-2- picrylhydrazyl (DPPH) using thin layer chromatography (TLC), then UV-Vis spectrophotometry λ517 nm with five variations of concentration. Results showed all 34 fungal isolates have antioxidant activities based on a yellowish-white color change after applying 0.002% DPPH solution of the TLC method and IC50 value of the UV-Vis spectrophotometer. The highest antioxidant activity was shown by Aspergillus austroafricanus MB 1 (IC50 = 12.08 ppm) from Bengkulu accession and A. oryzae MM 13 (IC50 = 10.29 ppm) from Malaysia accession. A. austroafricanus MB 1 produced more antioxidant compounds (seven) than A. oryzae MM 13 (six). The antioxidant compounds produced by both endophytic fungi included in the group of flavonoids, fatty acids, and carboxylic acids. The research implies that A. austroafricanus MB 1 and A. oryzae MM 13 could be further developed as sources of antioxidants.

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Section: Introductionmentioning

confidence: 99%