Diversity of fishes collected with light traps in the oldest marine protected area in Vietnam revealed by DNA barcoding

Pham, Manh Hung; Hoàng, Huy Đức; Panfili, Jacques; Ponton, Dominique; Durand, Jean‐Dominique

doi:10.1007/s12526-022-01266-4

Cited by 6 publications

(10 citation statements)

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“…This last result underlines the need for a curated reference barcode library for the eDNA analyses. Recently, Pham et al [46] used a DNA barcoding approach to identify juveniles or small crypto-benthic species collected in Con Dao (Vietnam) and estimated that up to 30% of the OTUs recovered in the samples could not be identified precisely due to species misidentification or mislabeled sequences in BOLD (the international DNA barcode library of the iBOL consortium). The taxonomic uncertainty concerning the DNA barcodes used for species identification is well-known and has already been flagged in numerous barcoding studies [46].…”

Section: Resultsmentioning

confidence: 99%

“…Recently, Pham et al [46] used a DNA barcoding approach to identify juveniles or small crypto-benthic species collected in Con Dao (Vietnam) and estimated that up to 30% of the OTUs recovered in the samples could not be identified precisely due to species misidentification or mislabeled sequences in BOLD (the international DNA barcode library of the iBOL consortium). The taxonomic uncertainty concerning the DNA barcodes used for species identification is well-known and has already been flagged in numerous barcoding studies [46]. In this context, BOLD proposes an interim taxonomic nomenclature to circumvent these taxonomical issues, by assigning all of the DNA barcodes available in the BOLD library with a barcoding index number (BIN) that correspond to an OTU [47].…”

Section: Resultsmentioning

confidence: 99%

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Fish Diversity along the Mekong River and Delta Inferred by Environmental-DNA in a Period of Dam Building and Downstream Salinization

et al. 2022

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The Mekong River is one of the largest rivers in the world and hosts the second greatest fish diversity in the world after the Amazon. However, despite the importance of this diversity and its associated biomass for human food security and the economy, different anthropogenic pressures threaten the sustainability of the Mekong River and fish diversity, including the intense damming of the main river. Both the increase in salt-water penetration into the Mekong Delta and the disrupted connectivity of the river may have serious impacts on the numerous freshwater and migratory species. To evaluate the potential of an eDNA approach for monitoring fish diversity, water was sampled at 15 sites along the salinity gradient in the Mekong Delta and along 1500 km of the main stream, from Vietnam to Thailand and Laos. A total of 287 OTUs were recovered, of which 158 were identified to the species level using both reference sequences available in GenBank and references obtained locally. Agglomerative hierarchical clustering and PCA identified up to three main species assemblages in our samples. If the transition from brackish to freshwater conditions represents the main barrier between two of these assemblages, more surprisingly, the two other assemblages were observed in the freshwater Mekong, with a spatial disjunction that did not match any biogeographic ecoregion or the Khone falls, the latter thought to be an important fish dispersion barrier. Between 60% and 95% of the freshwater species were potamodromous. This pioneer eDNA study in the Mekong River at this geographical and ecological scale clearly confirmed the potential of this approach for ecological and diversity monitoring. It also demonstrated the need to rapidly build an exhaustive Mekong fish barcode library to enable more accurate species’ assignment. More eDNA surveys can now be expected to better describe the ecological niche of different species, which is crucial for any models aimed at predicting the impact of future damming of the Mekong.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Fish Diversity along the Mekong River and Delta Inferred by Environmental-DNA in a Period of Dam Building and Downstream Salinization

et al. 2022

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“…As documented by Doherty (1987), Thorrold (1992) and Brogan (1994) in other areas, the present study confirmed that light traps are a useful tool to attract a large number of individuals and species in different habitats of the coastal region of Con Son Island. It was shown that light traps captured not only larvae and juveniles of different species, but also adults of small‐sized species, such as Spratelloides (Pham et al, 2020) and many crypto‐benthic species belonging to different fish families including Gobiidae, Tripterygiidae and Blenniidae (Pham et al, 2022). According to Costello et al (2017), light traps are effective for investigating some understudied components of marine biodiversity because they attract organisms in their young stages, which are generally difficult to catch using conventional gear.…”

Section: Discussionmentioning

confidence: 99%

“…(Bregmacerotidae) could be cryptobenthic fish that are difficult to catch with fishing gear or by visual surveys (Goatley & Brandl, 2017). The use of light traps captured the diversity and abundance of coastal fish species in Con Dao better (Pham et al, 2022), particularly the families Gobiidae, Pomacentridae, Apogonidae, Blenniidae and Tripterygiidae, which were previously not considered to be predominant families in Con Dao using classical nets (Nguyen & Nguyen, 1997; Nguyen & Mai, 2020). Similarly, the families Clupeidae and Blenniidae turned out to be the most abundant coastal fish in the different habitats when collected using light traps.…”

Section: Discussionmentioning

confidence: 99%

“…which has not been adequately explored to date (Collet et al, 2017;Pham et al, 2022). Fish larvae were identified solely based on their morphology but since fish undergo rapid changes throughout their early life stages, it was difficult to ensure identification was accurate (Victor et al, 2009;Matarese et al, 2011;Ko et al, 2013;Leis, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Spatial and temporal diversity of fish captured by light traps in various habitats in coastal waters in the oldest marine protected area in Vietnam and its implications for conservation

Pham,

Panfili,

Simier

et al. 2023

Aquatic Conservation

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Vietnam's oldest marine protected area, the Con Dao Archipelago, hosts a high diversity of fishes, but they are not taken into account in its management. Knowledge of the spatial and temporal diversity of fishes is critical to identify the ecological functions of protected habitats, including as nurseries and feeding grounds. One way to assess diversity is using light traps that target young fish stages. Fish were collected monthly from June 2016 to May 2017 using light traps in three main habitats: seagrass beds, coral reefs and the harbour. Morphometry and DNA barcoding were used to identify the species caught. A total of 11,293 fish were collected, of which 1,248 were barcoded and assigned to 158 species belonging to 81 genera and 34 families, evidence that coral reefs and seagrass beds in Con Dao marine protected area (MPA) are home to a highly diverse and abundant community of fish species. Species richness and Shannon diversity indices did not differ significantly among sampling sites, but both species composition and diversity indices differed in the wet and dry seasons. The abundance of fish was related to sea water temperatures, which varied significantly depending on the month. During the dry season, fish diversity peaked in April, when the water temperature was highest. Conserving fish in Con Dao MPA is important not only for the characterization of the trophic network in coral reefs and seagrass beds but also for preservation of coral reef ecosystems and endangered species. Ensuring connectivity between coral reefs and seagrass beds in Con Dao MPA would facilitate fish conservation but, at present, the MPA only focuses on protecting fragmented habitats that are home to a number of endangered mammal species. Local authorities should establish a continuum of protected areas along coastal habitats and seasonal controls on fishing as part of sustainable management of MPAs.

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Cusk-eel confusion: revisions of larval Luciobrotula and Pycnocraspedum and re-descriptions of two bythitid larvae (Ophidiiformes)

et al. 2023

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Diversity of fishes collected with light traps in the oldest marine protected area in Vietnam revealed by DNA barcoding

Cited by 6 publications

References 84 publications

Fish Diversity along the Mekong River and Delta Inferred by Environmental-DNA in a Period of Dam Building and Downstream Salinization

Fish Diversity along the Mekong River and Delta Inferred by Environmental-DNA in a Period of Dam Building and Downstream Salinization

Spatial and temporal diversity of fish captured by light traps in various habitats in coastal waters in the oldest marine protected area in Vietnam and its implications for conservation

Cusk-eel confusion: revisions of larval Luciobrotula and Pycnocraspedum and re-descriptions of two bythitid larvae (Ophidiiformes)

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