Diversity ofBacillus cereusgroup strains is reflected in their broad range of pathogenicity and diverse ecological lifestyles

Ceuppens, Siele; Boon, Nico; Uyttendaele, Mieke

doi:10.1111/1574-6941.12110

Cited by 175 publications

(146 citation statements)

References 171 publications

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“…B. cereus is an aerobic spore-forming Gram-positive bacterium normally disseminated in the environment. It is usually isolated from the soil, plant materials, raw meat and processed meat products (Carlin F et al, 2010;Ceuppens et al, 2013). Schedule identification of B. cereus is generally comprised isolation on selective media, revealing of motility, hemolysis prototype on blood agar, and acidification of glucose (Stenfors Arnesen et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and antimicrobial resistance of Bacillus cereus isolated from beef products in Egypt

Shawish¹,

Tarabees²

2017

Open Vet J.

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Foodborne pathogens have the main concern in public health and food safety. Bacillus cereus food poisoning is one of the most important foodborne pathogens worldwide. In the present study, a total of 200 random beef product samples were collected from different supermarkets located at Menofia and Cairo governorates were examined for the presence of B. cereus. In addition, the presence of some virulence encoding genes was evaluated using Multiplex PCR. Finally, the antibiogram testing was conveyed to illustrate the resistance pattern of the confirmed B. cereus. The data showed that B. cereus was recovered from 22.5%, 30%, 25%, 37.5% and 15% of the minced meat, burger, sausage, kofta, and luncheon respectively. Among the 20 examined isolates 18/20 (90%) were harbor hblC enterotoxin encoding gene compared with 20/20 (100) were have cytK enterotoxin encoding gene. The isolated strains of B. cereus were resistant to penicillin G and sensitive to oxacillin, clindamycin, vancomycin, erythromycin, gentamicin, ciprofloxacin, and ceftriaxone. In all, the obtained data showed the importance of emerging B. cereus in disease control and prevention programs, and in regular clinical and food quality control laboratories in Egypt.

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Section: Introductionmentioning

confidence: 99%

Prevalence and antimicrobial resistance of Bacillus cereus isolated from beef products in Egypt

Shawish¹,

Tarabees²

2017

Open Vet J.

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“…Bacillus cereus is a facultative anaerobic, motile, grampositive, catalase positive, does not ferment mannitol, rod-shape, beta-hemolytic and spore-forming bacterium; that is widely distributed in the environment due to its ability to resist hostile conditions (Ceuppens et al 2013) and growth temperature ranging from 10 to 48°C with optimal growth between 28°C and 35°C, pH values of 4.9 to 9.3 and water activities of 0.92 to 1.0 (OSPBH 2005).…”

Section: Introductionmentioning

confidence: 99%

Bacillus Cereus isolation and load from raw cow milk sold in Markets of Haramaya District, eastern Ethiopia

et al. 2017

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Background: Bacillus cereus is a spore-forming bacterium that is widely distributed in the environment and can be detected in a variety of raw milk and milk products especially in vegetative form, which exposed directly in contact with the soil and produces toxins which caused food borne illness and considered as a significant public health hazard. A cross-sectional study was carried out to determine the prevalence and load of Bacillus cereus vegetative cells in raw bulk milk sold in market places at Bate, Haramaya and Aweday Towns, Eastern Ethiopia as well as to assess the in-vitro antimicrobial susceptibility of isolates. A total of 103 raw milk samples were collected and examined for the presence of Bacillus cereus by using Bacillus cereus selective agar media. The isolated presumptive colonies were further analyzed using standard microbiological methods to confirm the presence of Bacillus cereus. Spreading plate method was employed to count the bacteria from milk samples based on 10 fold serial dilutions. For antimicrobial susceptibility test, the disc diffusion test was employed using commercially available 10 antimicrobial discs. Results: The overall prevalence's of Bacillus cereus in raw milk samples was 38.8%. Milk collected from Aweday market showed higher occurrence (46.9%) than from Bate (40%) and Haramaya town (31.7%). Analysis of bacterial count showed that, 60% of Bacillus cereus positive milk samples were with bacterial load above recommended limit (>10

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“…Bacillus cereus is broadly disseminated in the environment because of its ability to withstand adversarial conditions; it is a facultative anaerobic, Gram-positive, spore-forming bacteria (Carlin et al, 2010;Ceuppens et al, 2013). B. cereus is a prevailing food contaminant; it can be found in various types of raw food such as rice, meat, vegetables, raw milk, and dairy products in addition to cooked meals (Eglezos et al, 2010;Samapundo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Detection of B. Cereus and Some of Its Virulence Genes in Some Dairy Desserts and Children Diarrhea

El‐Zamkan¹,

Mubarak²

2017

AJVS

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Key words: B. cereus, Dairy desserts, Diarrhea and virulence genesB. cereus is an opportunistic foodborne pathogen that is found in most of the cases to be responsible for food-borne outbreaks. This study aimed to detect and quantify B. cereus in dairy desserts that locally manufactured and sold in different localities in Qena city, also to take an image about the role that is may be played by these products in causing foodborne illness by detection of B. cereus in diarrheal samples collected from the same regions from which the food samples are obtained. A total of 120 samples of ice-cream, mahallbia, rice and milk and diarrhea (30 samples each) were examined for presence of B. cereus. The results obtained in this study revealed detection of B. cereus in 55.6 and 40% of dairy dessert and diarrhea samples, respectively. B. cereus could be counted in 30, 60 and 76.7% of ice-cream, mahallbia and rice and milk samples with mean values of 3.5× 10 4 ±1.5× 10 4 , 6.3×10 5 ±1.9×10 5 and 1.4×10 7 ±6.6× 10 6 CFU/g, respectively. The possibility of involvement of theses dairy dessert samples in food poisoning cases was reinforced especially after detection the virulence genes of B. cereus isolates. It was found that 80, 44 and 24% of B. cereus isolates recovered from food samples harbored nhe, hbl and cytK genes, receptively including 55.6, 33.3 and 0% for ice-cream isolates; 83.3, 50 and 44.4% for mahallbia isolates and 86.9, 43.5 and 17.4% for rice and milk isolates. While for diarrhea samples, 75% of B. cereus isolates obtained from diarrhea samples carried nhe and cytK, respectively and 50% were found to be positive for hbl gene. Neither of the isolates that obtained from dairy dessert nor those obtained from diarrhea samples was positive for presence of ces gene.

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Diversity ofBacillus cereusgroup strains is reflected in their broad range of pathogenicity and diverse ecological lifestyles

Cited by 175 publications

References 171 publications

Prevalence and antimicrobial resistance of <i>Bacillus cereus</i> isolated from beef products in Egypt

Prevalence and antimicrobial resistance of <i>Bacillus cereus</i> isolated from beef products in Egypt

Bacillus Cereus isolation and load from raw cow milk sold in Markets of Haramaya District, eastern Ethiopia

Detection of B. Cereus and Some of Its Virulence Genes in Some Dairy Desserts and Children Diarrhea

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