Diversity of <i>Claviceps paspali</i> reveals unknown lineages and unique alkaloid genotypes

Oberti, H; Rizza, Marco Dalla; Reyno, Rafael; Murchio, S.; Altier, Nora; Abreo, Eduardo

doi:10.1080/00275514.2019.1694827

Cited by 11 publications

(13 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…C. paspali ILB388 isolated from P. plicatulum [ 52 ] was obtained from the “Laboratorio de Bioproducción” at INIA Las Brujas Fungal Collection (ILB). Vegetative mycelium was harvested form cultures kept in Claviceps medium [ 95 ] for 1 wk.…”

Section: Methodsmentioning

confidence: 99%

“…Here, we used in silico prediction to identify possible secreted proteins and pathogenicity factor-like proteins present in the genomes of three isolates of C. paspali belonging to different lineages [ 52 , 53 ] within the section Paspalorum [ 1 ]. Then, we used in planta RNA-seq during the early stages of infection of a P. dilatatum susceptible cultivar by C. paspali to pinpoint the expressed secretome.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide analysis of Claviceps paspali: insights into the secretome of the main species causing ergot disease in Paspalum spp

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background The phytopatogen Claviceps paspali is the causal agent of Ergot disease in Paspalum spp., which includes highly productive forage grasses such as P. dilatatum. This disease impacts dairy and beef production by affecting seed quality and producing mycotoxins that can affect performance in feeding animals. The molecular basis of pathogenicity of C. paspali remains unknown, which makes it more difficult to find solutions for this problem. Secreted proteins are related to fungi virulence and can manipulate plant immunity acting on different subcellular localizations. Therefore, identifying and characterizing secreted proteins in phytopathogenic fungi will provide a better understanding of how they overcome host defense and cause disease. The aim of this work is to analyze the whole genome sequences of three C. paspali isolates to obtain a comparative genome characterization based on possible secreted proteins and pathogenicity factors present in their genome. In planta RNA-seq analysis at an early stage of the interaction of C. paspali with P. dilatatum stigmas was also conducted in order to determine possible secreted proteins expressed in the infection process. Results C. paspali isolates had compact genomes and secretome which accounted for 4.6–4.9% of the predicted proteomes. More than 50% of the predicted secretome had no homology to known proteins. RNA-Seq revealed that three protein-coding genes predicted as secreted have mayor expression changes during 1 dpi vs 4 dpi. Also, three of the first 10 highly expressed genes in both time points were predicted as effector-like. CAZyme-like proteins were found in the predicted secretome and the most abundant family could be associated to pectine degradation. Based on this, pectine could be a main component affected by the cell wall degrading enzymes of C. paspali. Conclusions Based on predictions from DNA sequence and RNA-seq, unique probable secreted proteins and probable pathogenicity factors were identified in C. paspali isolates. This information opens new avenues in the study of the biology of this fungus and how it modulates the interaction with its host. Knowledge of the diversity of the secretome and putative pathogenicity genes should facilitate future research in disease management of Claviceps spp.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of Claviceps paspali: insights into the secretome of the main species causing ergot disease in Paspalum spp

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…C. paspali ILB432 was isolated from sclerotia obtained from Paspalum urvillei -infected inflorescences collected near Portezuelo, Maldonado, Uruguay (global positioning system coordinates 34.888591S, 55.030316W), using a previously reported C. paspali isolation procedure ( 5 ). ILB432 was cultured at 26°C on Claviceps medium containing 36 g/liter potato dextrose agar, 2 g/liter yeast extract, 10 g/liter malt extract, 10 g/liter sucrose, and 5 g/liter agar ( 6 ).…”

Section: Announcementmentioning

confidence: 99%

“…ILB432 was cultured at 26°C on Claviceps medium containing 36 g/liter potato dextrose agar, 2 g/liter yeast extract, 10 g/liter malt extract, 10 g/liter sucrose, and 5 g/liter agar (6). Phylogenetic analysis based on the partial gene coding for the second largest subunit of RNA polymerase subunit II (RPB2) showed that ILB432, like reference isolate RRC-1481, belongs to the most frequent lineage of C. paspali (5).…”

mentioning

confidence: 99%

New Draft Genome Sequence of the Ergot Disease Fungus Claviceps paspali

Oberti

Abreo

Reyno

et al. 2020

Microbiol Resour Announc

Self Cite

View full text Add to dashboard Cite

show abstract

“…Most countries have implemented regulations to limit the concentration of mycotoxins in food and feed products due to their implications for commerce and public health. National information is reported for almost every agriculturally important mycotoxin, including deoxynivalenol, trichothecenes, zearalenone, fumonisin, aflatoxin, ochratoxin and alkaloids (19)(20)(21)(22)(23)(24) (25) .…”

Section: One Health From the Agricultural Sciences Perspectivementioning

confidence: 99%

One health: considerations in the International Year of Plant Health

Altier

Abreo

2020

Agrociencia

View full text Add to dashboard Cite

The concept of “one health” is gaining strength at a global scale. The term summarizes a notion that has been known for more than a century: human and animal health are interdependent and linked to the ecosystems in which they coexist. In the International Year of Plant Health (2020), it is crucial that the agricultural sciences call attention for the need to consider the health of plants and their microbiome as a whole within the “one health” concept, closely linked to the health of the soil, the environment, animals and humans. In the context of agroecological production based on the sustainable management of natural resources, integrated pest management has been promoted to reduce the use of pesticides and mitigate negative effects on human and environmental health. The combination of genetic resistance with cultural and biological control have contributed to achieving this goal. The management of the microbiome offers also the possibility of addressing plant nutritional issues and the continuum between environmental, plant, animal and human health. With its strategic plan and long-term vision, INIA plays an essential role in the generation of knowledge and technologies, acting in accordance to the one health approach.

show abstract

Diversity of Claviceps paspali reveals unknown lineages and unique alkaloid genotypes

Cited by 11 publications

References 43 publications

Genome-wide analysis of Claviceps paspali: insights into the secretome of the main species causing ergot disease in Paspalum spp

Genome-wide analysis of Claviceps paspali: insights into the secretome of the main species causing ergot disease in Paspalum spp

New Draft Genome Sequence of the Ergot Disease Fungus Claviceps paspali

One health: considerations in the International Year of Plant Health

Contact Info

Product

Resources

About