Diversity of laccase genes from basidiomycetes in a forest soil

Luis, Patricia; Walther, Grit; Kellner, Harald; Martin, Francis; Buscot, François

doi:10.1016/j.soilbio.2004.02.017

Cited by 137 publications

(109 citation statements)

References 33 publications

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“…1). Soil DNAs were extracted following the protocol of the Fast DNA Spin Kit for Soil (Qbiogene), adding 10 washes with 5.5 M guanidine thiocyanate (Luis et al, 2004). Two replicate samples of 0.5 g were extracted and pooled for amplification.…”

Section: Sampling and Dna Extractionmentioning

confidence: 99%

Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatumâtruffle-ground

Zampieri

Murat

Cagnasso

et al. 2010

FEMS Microbiology Ecology

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Truffles are hypogeous ectomycorrhizal fungi. They belong to the genus Tuber and are currently considered a hot spot in fungal biology due to their ecological and economic relevance. Among all the species, Tuber magnatum is the most appreciated because of its special taste and aroma. The aim of this work was to set up a protocol to detect T. magnatum in soil and to assess its distribution in a natural truffle-ground. We used the β-tubulin gene as a marker to identify T. magnatum in the soil. This gene allowed us to trace the distribution of the fungus over the entire truffle-ground. Tuber magnatum was found, in one case, 100 m from the productive host plant. This study highlights that T. magnatum mycelium is more widespread than can be inferred from the distribution of truffles and ectomycorrhizas. Interestingly, a new haplotype -never described from fruiting body material -was identified. The specific detection of T. magnatum in the soil will allow to unravel the ecology of this fungus, following its mycelial network. Moreover, this new tool may have practical importance in projects aimed to increase large-scale truffle production, checking for T. magnatum persistence in plantations.

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Section: Sampling and Dna Extractionmentioning

confidence: 99%

Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatumâtruffle-ground

Zampieri

Murat

Cagnasso

et al. 2010

FEMS Microbiology Ecology

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“…The saprotrophic fungi play major roles in the decomposition of dead plant material, particularly cellulose and lignin in the litter and organic soil horizons (Luis et al, 2004;O'Brien et al, 2005), and are thought to be very diverse in lowland tropical soils (Bills and Polishook, 1994). Mycorrhizal fungi are mutuallistic symbionts of plants (Malloch et al, 1980), and are especially abundant in the O and A soil horizons (Luis et al, 2004;O'Brien et al, 2005) and are now being shown to play important roles in both providing materials to plants and in organic C and N harvesting from surrounding soil regions (Talbot et al 2008). The observations from the soil analyses in www.intechopen.com our study were consistent with a more fungal-dominant soil ecosystem in the PM-L soils.…”

Section: Discussionmentioning

confidence: 99%

The Effects of the N-Fixing Tree Pentaclethra macroloba on the Above and Below Ground Communities Within a Primary Forest in the Northern Zone of Costa Rica

Eaton¹,

Shebitz²,

Niemiera³

et al. 2012

The Functioning of Ecosystems

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“…DNA was extracted from soil samples using the FastDNA Spin Kit for Soil (Qbiogene, Heidelberg, Germany) with the modifications described by Luis et al (2004 Luis, P, Walther, G, Kellner, H, Martin, F andBuscot, F. 2004. Diversity of laccase genes from basidiomycetes in a forest soil.…”

Section: Dna Extraction and Pcrmentioning

confidence: 99%

Bacterial and fungal communities associated withTuber magnatum‐productive niches

Mello

Miozzi

Vizzini

et al. 2010

Plant Biosystems - An International Journal Dealing with all As

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Truffles are hypogeous ectomycorrhizal fungi of ecological interest for forestry in soils of the northern hemisphere, and of economical relevance for food markets worldwide. The molecular mechanisms that control truffle body formation are largely unknown, as well as the environmental factors that are likely involved. Among the latter, it has been hypothesized that soil-borne communities may have an impact on truffle production. To address this question, we investigated bacterial and fungal communities resident in productive versus adjacent non-productive grounds of the white truffle Tuber magnatum by using PCR-DGGE. Although bacterial communities were generally highly similar across all samples within the grounds, profiles did cluster according to the productivity of circumscribed niches, and a Moraxella osloensis population appeared to be associated with productive sites. Fungal communities revealed several populations, yet showed no obvious patterns in relation to productivity, although Mortierella and Fusarium oxysporum appeared to be more abundant in the productive area. Our results offer a first glimpse into microbial communities thriving in truffle productive niches, and open the question as to whether microbemediated mechanisms may facilitate/inhibit truffle fruiting-body production or, vice versa, i.e. whether truffle sporocarps have an impact on the microbes living in the rhizosphere.

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Diversity of laccase genes from basidiomycetes in a forest soil

Cited by 137 publications

References 33 publications

Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatumâtruffle-ground

Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatumâtruffle-ground

The Effects of the N-Fixing Tree Pentaclethra macroloba on the Above and Below Ground Communities Within a Primary Forest in the Northern Zone of Costa Rica

Bacterial and fungal communities associated withTuber magnatum‐productive niches

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Diversity of laccase genes from basidiomycetes in a forest soil

Cited by 137 publications

References 33 publications

Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatumâtruffle-ground

Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatumâtruffle-ground

The Effects of the N-Fixing Tree Pentaclethra macroloba on the Above and Below Ground Communities Within a Primary Forest in the Northern Zone of Costa Rica

Bacterial and fungal communities associated withTuber magnatum‐productive niches

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Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatumâtruffle-ground

Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatumâtruffle-ground