Diversity of lactic acid bacteria in two Flemish artisan raw milk Gouda-type cheeses

Multifactorial diversity sustains microbial community stability

“…Similarly, milk can accommodate numerous microbial species (Delbes et al, 2007). However, strong selective pressures like heat treatment (Van Hoorde et al, 2008), dairy processing conditions (Randazzo et al, 2002), intrinsic properties of lactic acid fermentation and antagonistic interactions (Lindgren and Dobrogosz 1990), enrich the microbiota toward a few lactic acid bacteria species.…”

PCR-DGGE for the profiling of cheese bacterial communities: strengths and weaknesses of a poorly explored combined approach

“…In the following years, the double PCR-DGGE approach was applied in a few investigations aimed at identifying the microbial diversity of raw milk cheeses, namely, Stilton (Ercolini et al 2003), water buffalo Mozzarella ), Goudatype cheeses (Van Hoorde et al 2008, 2010, an artisanal fresh sheep cheese produced in a limited area of eastern Croatia (Pogačić et al 2011) and traditional Italian semihard cheeses (Aquilanti et al 2013;Ricciardi et al 2014). However, in all these investigations, the bulk approach consisted in the sole analysis of a few selected plates, namely, the countable plates (Ercolini et al 2003Pogačić et al 2011), the plates inoculated with the sole lowest dilutions (−1) (Van Hoorde et al 2008) and the plates inoculated with both the lowest and the highest dilutions (Aquilanti et al 2013;Ricciardi et al 2014). …”

Application of PCR-Based Methods to Dairy Products and to Non-Dairy Probiotic Products

“…Denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE) and temporal temperature gradient gel electrophoresis (TTGE) are widely used to study cheese microbial communities Ercolini et al, 2001;Ogier et al, 2002;Randazzo et al, 2002;Ercolini et al, 2003;Mauriello et al, 2003;Andrighetto et al, 2004;Cocolin et al, 2004;Ercolini et al, 2004;Henri-Dubernet et al, 2004;Lafarge et al, 2004;Ogier et al, 2004;Rantsiou et al, 2004;Le Bourhis et al, 2005;Flórez and Mayo, 2006;Randazzo et al, 2006;Cocolin et al, 2007;El-Baradei et al, 2007;Le Bourhis et al, 2007;Parayre et al, 2007;Abriouel et al, 2008;Bonetta et al, 2008;Ercolini et al, 2008;Gala et al, 2008;Henri-Dubernet et al, 2008;Nikolic et al, 2008;Rantsiou et al, 2008b;Van Hoorde et al, 2008;Alegría et al, 2009;Casalta et al, 2009;Dolci et al, 2009;Giannino et al, 2009;Serhan et al, 2009;Dolci et al, 2010;Fontana et al, 2010;Fuka et al, 2010;Randazzo et al, 2010;Van Hoorde et al, 2010;Masoud et al, 2011). Target sequences from rRNA or housek...…”

“…Most methods described in the literature involve prior separation of the cells (Allmann et al, 1995;Herman et al, 1997;Serpe et al, 1999;Torriani et al, 1999;McKillip et al, 2000;Coppola et al, 2001;Ogier et al, 2002;Randazzo et al, 2002;Ercolini et al, 2003;Furet et al, 2004;Ogier et al, 2004;Baruzzi et al, 2005;Rudi et al, 2005;Rademaker et al, 2006;ElBaradei et al, 2007;Lopez-Enriquez et al, 2007;Parayre et al, 2007;Rossmanith et al, 2007;Trmcic et al, 2008;Van Hoorde et al, 2008;Alegría et al, 2009;Dolci et al, 2009;Zago et al, 2009;Le Dréan et al, 2010;Mounier et al, 2010). The recovery of cells from milks or fermented milks is easier to perform than from cheeses.…”

“…The hypervariable regions are flanked by conserved sequences, which can serve for amplification with "universal" primers (Baker et al, 2003). The variable V1 Bonetta et al, 2008), V3 Ercolini et al, 2001;Ogier et al, 2002;Duthoit et al, 2003;Ercolini et al, 2003;Mauriello et al, 2003;Andrighetto et al, 2004;Ercolini et al, 2004;Feurer et al, 2004a;Feurer et al, 2004b;Lafarge et al, 2004;Ogier et al, 2004;Duthoit et al, 2005;Flórez and Mayo, 2006;Delbes et al, 2007;El-Baradei et al, 2007;Parayre et al, 2007;Abriouel et al, 2008;Ercolini et al, 2008;Gala et al, 2008;Van Hoorde et al, 2008;Alegría et al, 2009;Casalta et al, 2009;Dolci et al, 2009;Giannino et al, 2009;Mounier et al, 2009;Serhan et al, 2009;Dolci et al, 2010;Fontana et al, 2010;Van Hoorde et al, 2010;Masoud et al, 2011), V2 (Duthoit et al, 2003;Delbes and Montel, 2005;Saubusse et al, 2007), V4-V5 , V1-V3 (Randazzo et al, 2002), V4-V8 (Randazzo et al, 2006), V5-V6 (Le Bourhis et al, 2005;Le Bourhis et al, 2007) and V6-V8 …”