Diversity of Micromonospora strains isolated from nitrogen fixing nodules and rhizosphere of Pisum sativum analyzed by multilocus sequence analysis

Three actinobacterial strains, CR30T, CR36 and CR38T, were isolated from rhizosphere soil of Pisum sativum plants collected in Spain. The strains were filamentous, Gram-stain-positive and produced single spores. Phylogenetic, chemotaxonomic and morphological analyses confirmed that the three strains belonged to the genus Micromonospora . 16S rRNA gene sequence analysis of strains CR30T and CR36 showed a close relationship to Micromonospora coriariae NAR01T (99.3 % similarity) while strain CR38T had a similarity of 99.0 % with Micromonospora saelicesensis Lupac 09T. In addition, gyrB gene phylogeny clearly differentiated the novel isolates from recognized Micromonospora species. DNA–DNA hybridization, BOX-PCR and ARDRA profiles confirmed that these strains represent novel genomic species. The cell-wall peptidoglycan of strains CR30T and CR38T contained meso-diaminopimelic acid. Both strains had MK-10(H4) as the main menaquinone and a phospholipid type II pattern. An array of physiological tests also differentiated the isolates from their closest neighbours. Considering all the data obtained, it is proposed that strains CR30T and CR36 represent a novel species under the name Micromonospora cremea sp. nov. (type strain CR30T = CECT 7891T = DSM 45599T), whereas CR38T represents a second novel species, for which the name Micromonospora zamorensis sp. nov. is proposed, with CR38T ( = CECT 7892T = DSM 45600T) as the type strain.

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“…T and CR36 were more closely related to M. coriariae NAR01 T than to M. chokoriensis 2-19 (6) T (Carro et al, 2012).…”

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confidence: 84%

Micromonospora cremea sp. nov. and Micromonospora zamorensis sp. nov., isolated from the rhizosphere of Pisum sativum

Carro

Pukall

Spröer

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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“…To analyse the diversity of Micromonospora strains isolated from Pisum sativum nodules, the multilocus sequence analysis (MLSA) technique was used in a previous study (Carro et al, 2012b). From the MLSA data, several strains were underpinned as potential representatives of novel species.…”

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confidence: 99%

“…The almost complete 16S rRNA gene sequences obtained previously (Carro et al, 2012b) were compared with those of species with validly published names using the Ez-Taxon server (Kim et al, 2012). Strains GUI23 T , GUI43 T and GUI63 T were closely related to Micromonospora saelicesensis Lupac 09 T , showing 99.6, 99.2 and 99.4 % sequence similarity respectively.…”

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confidence: 99%

“…In a previous study, Carro et al (2012b) carried out DNA-DNA hybridization analyses (DDH) between the novel isolates and their closest phylogenetic neighbour, M. saelicesensis Lupac 09 T . The strains showed DDH values below 70 %, namely 56.9, 62.8 and 57.9 % for GUI23 T , GUI43 T and GUI63 T , respectively.…”

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confidence: 99%

“…The strains showed DDH values below 70 %, namely 56.9, 62.8 and 57.9 % for GUI23 T , GUI43 T and GUI63 T , respectively. In addition, isolate GUI63 T was hybridized with M. zamorensis CR38 T (16S rRNA gene sequence similarity 99.3 %) as this strain appeared closer on the 16S rRNA gene phylogenetic tree; the resultant value was 46.6 % (Carro et al, 2012b). Similarly, the DNA relatedness values among the three isolates were below the threshold value for assigning strains to the same species (Wayne et al, 1987).…”

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confidence: 99%

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Micromonospora ureilytica sp. nov., Micromonospora noduli sp. nov. and Micromonospora vinacea sp. nov., isolated from Pisum sativum nodules

Carro

Riesco

Spröer

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Actinobacteria and Their Role as Plant Probiotics

Menéndez

Carro

2019

Soil Biology

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Actinobacteria is one of the largest phyla within the domain Bacteria. This phylum comprises more than 400 genera heterogeneously distributed in up to 50 families, 20 orders and 6 classes, being composed with very diverse groups of microorganisms. Members included within this phylum were recovered from a wide range of aquatic and terrestrial environments and also from a huge number of higher organisms, including plants. Actinobacteria inhabiting soils and plants are well known as producers of bioactive molecules and as biocontrol agents, possessing antimicrobial activities mostly against pathogenic fungi and/or bacteria. Moreover, some of them have the capacity to exert beneficial effects on plant growth and development via different plant growth-promoting mechanisms, i.e., phytohormones biosynthesis, siderophore production, and phosphate solubilization, among others. The available genomic data revealed that members belonging to this phylum have a huge potential as Plant Probiotic Actinobacteria. A plethora of studies reported the isolation and identification of plant endophytic actinobacteria possessing those features and also their performance under controlled conditions. However, few studies show the effects of the inoculation of these actinobacteria on real field conditions. In this chapter, we will provide an overview of the available data on the Actinobacteria displaying plant growth-promoting features, particularly in the ones that already had applications in agriculture. Together with a correct taxonomic classification, we will present evidence that the Plant Probiotic Actinobacteria should be considered as a source of bacterial candidates that will be important for a future sustainable agriculture.

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Diversity of Micromonospora strains isolated from nitrogen fixing nodules and rhizosphere of Pisum sativum analyzed by multilocus sequence analysis

Cited by 75 publications

References 30 publications

Micromonospora cremea sp. nov. and Micromonospora zamorensis sp. nov., isolated from the rhizosphere of Pisum sativum

Micromonospora cremea sp. nov. and Micromonospora zamorensis sp. nov., isolated from the rhizosphere of Pisum sativum

Micromonospora ureilytica sp. nov., Micromonospora noduli sp. nov. and Micromonospora vinacea sp. nov., isolated from Pisum sativum nodules

Actinobacteria and Their Role as Plant Probiotics

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