Diversity of nodular bacteria ofScorpiurus muricatusin western Algeria and their impact on plant growth

Abstract

A total of 51 bacterial strains were isolated from root nodules of Scorpiurus muricatus sampled from 6 regions of western Algeria. Strain diversity was assessed by rep-PCR amplification fingerprinting, which grouped the isolates into 28 different clusters. Partial nucleotide sequencing of the 16S rRNA gene and BLAST analysis revealed that root nodules of S. muricatus were colonized by different species close to Rhizobium vignae, Rhizobium radiobacter, Rhizobium leguminosarum, Phyllobacterium ifriqiyense, Phyllobacterium endophyticum, Starkeya sp., and Pseudomonas sp. However, none of these strains was able to form nodules on its host plant; even nodC was present in a single strain (SMT8a). The inoculation test showed a great improvement in the growth of inoculated plants compared with noninoculated control plants. A significant amount of indole acetic acid was produced by some strains, but only 2 strains could solubilize phosphate. In this report we described for the first time the diversity of bacteria isolated from root nodules of S. muricatus growing in different regions in western Algeria and demonstrated their potential use in promoting plant growth.

Mecanismos de inducción de rizobios para reducir el estrés por sequía en las leguminosas

“…La producción del AIA por rizobios asociados a leguminosas tolerantes a condiciones de sequía fue informada por varios autores como Bouchiba et al (2017), quienes señalaron a Rhizobium vignae SMB23 aislados de nódulos de Scorpiurus muricatus, productor de 135 g/mL, del AIA, Defez et al (2017) mencionaron la eficiencia de Ensifer meliloti (Ms-RD64) sobreproductora de AIA, en la mejora del estrés hídrico en Medicago sativa. Asimismo, Ullah et al (2017), indicaron a Rhizobium leguminosarum y Mesorhizobium ciceri productores del AIA, con capacidad de inducir tolerancia a la sequía en plantas de trigo.…”

Phylogenomic Review of Root Nitrogen-Fixing Symbiont Population Nodulating Northwestern African Wild Legumes

“…Rejili et al [51] reported that Lotus creticus microsymbiotes are closely related to R. huautlense in the arid areas of Tunisia. Bacterial strains isolated from root nodules of Scorpiurus muricatus sampled from different regions of western Algeria are affiliated to R. vignae, R. radiobacter, and R. leguminosarum [60]. For the Trifolieae tribe, R. galegae species was isolated, in Tunisia and Algeria, from Medicago marima and M. truncatula [31].…”