Diversity of Odorant-binding Proteins and Chemosensory Proteins in Insects

Pelosi, Paolo

doi:10.1093/chemse/bjh229

Cited by 206 publications

(183 citation statements)

References 20 publications

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“…Insect OBPs are small, water soluble molecules expressed in both olfactory and gustatory sensilla, as well as in other specialised tissues (Pelosi et al, 2005). OBPs have also been found in non-olfactory tissue, suggesting that their roles may be related to general carrier capabilities with broad specificity for lipophilic compounds (Forêt and Maleszka, 2006).…”

Section: Odorant-binding Protein Familymentioning

confidence: 99%

An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas’ disease

Assumpção

Francischetti

Andersen

et al. 2008

Insect Biochemistry and Molecular Biology

117

132

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Triatoma infestans is a hemiptera, vector of Chagas' disease, that feeds exclusively on vertebrate blood in all life stages. Hematophagous insects' salivary glands (SG) produce potent pharmacological compounds that counteract host hemostasis, including anti-clotting, anti-platelet, and vasodilatory molecules. To obtain a further insight into the salivary biochemical and pharmacological complexity of this insect, a cDNA library from its salivary glands was randomly sequenced. Also, salivary proteins were submitted to two dimentional gel (2D-gel) electrophoresis followed by MS analysis. We present the analysis of a set of 1,534 (SG) cDNA sequences, 645 of which coded for proteins of a putative secretory nature. Most salivary proteins described as lipocalins matched peptide sequences obtained from proteomic results.

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Section: Odorant-binding Protein Familymentioning

confidence: 99%

An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas’ disease

Assumpção

Francischetti

Andersen

et al. 2008

Insect Biochemistry and Molecular Biology

117

132

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“…Moderate structural similarity to the odorant-binding protein (OBP) family and histochemical localization to the sensillum lymph of some species led to the suggestion that, like OBPs, CSPs function in olfaction and gustation by transporting hydrophobic ligands in the sensillum lymph (Pelosi et al 2005;. However, CSPs tend to be broadly expressed in tissues that lack sensilla, and their function remains uncertain.…”

Section: Introductionmentioning

confidence: 99%

RNAi-induced phenotypes suggest a novel role for a chemosensory protein CSP5 in the development of embryonic integument in the honeybee (Apis mellifera)

et al. 2007

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Small chemosensory proteins (CSPs) belong to a conserved, but poorly understood, protein family found in insects and other arthropods. They exhibit both broad and restricted expression patterns during development. In this paper, we used a combination of genome annotation, transcriptional profiling and RNA interference to unravel the functional significance of a honeybee gene (csp5) belonging to the CSP family. We show that csp5 expression resembles the maternal-zygotic pattern that is characterized by the initiation of transcription in the ovary and the replacement of maternal mRNA with embryonic mRNA. Blocking the embryonic expression of csp5 with double-stranded RNA causes abnormalities in all body parts where csp5 is highly expressed. The treated embryos show a "diffuse", often grotesque morphology, and the head skeleton appears to be severely affected. They are 'unable-to-hatch' and cannot progress to the larval stages. Our findings reveal a novel, essential role for this gene family and suggest that csp5 (unable-to-hatch) is an ectodermal gene involved in embryonic integument formation. Our study confirms the utility of an RNAi approach to functional characterization of novel developmental genes uncovered by the honeybee genome project and provides a starting point for further studies on embryonic integument formation in this insect.

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“…5B). OBPs and SAPs are soluble proteins that facilitate the activation of olfactory receptors by transporting odor molecules through the mucous layer to the receptors in the olfactory membrane (24,75,76). These data suggest that changes in OBP levels may modify the sensitivity of the olfactory sensilla, located primarily in the antennae, maxillary palps, and proboscis.…”

mentioning

confidence: 96%

Genome-wide profiling of diel and circadian gene expression in the malaria vector Anopheles gambiae

Rund

Hou

Ward

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

174

269

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Anopheles gambiae, the primary African vector of malaria parasites, exhibits numerous rhythmic behaviors including flight activity, swarming, mating, host seeking, egg laying, and sugar feeding. However, little work has been performed to elucidate the molecular basis for these daily rhythms. To study how gene expression is regulated globally by diel and circadian mechanisms, we have undertaken a DNA microarray analysis of An. gambiae under light/ dark cycle (LD) and constant dark (DD) conditions. Adult mated, non-blood-fed female mosquitoes were collected every 4 h for 48 h, and samples were processed with DNA microarrays. Using a cosine wave-fitting algorithm, we identified 1,293 and 600 rhythmic genes with a period length of 20-28 h in the head and body, respectively, under LD conditions, representing 9.7 and 4.5% of the An. gambiae gene set. A majority of these genes was specific to heads or bodies. Examination of mosquitoes under DD conditions revealed that rhythmic programming of the transcriptome is dependent on an interaction between the endogenous clock and extrinsic regulation by the LD cycle. A subset of genes, including the canonical clock components, was expressed rhythmically under both environmental conditions. A majority of genes had peak expression clustered around the day/night transitions, anticipating dawn and dusk. Genes cover diverse biological processes such as transcription/translation, metabolism, detoxification, olfaction, vision, cuticle regulation, and immunity, and include rate-limiting steps in the pathways. This study highlights the fundamental roles that both the circadian clock and light play in the physiology of this important insect vector and suggests targets for intervention.

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Diversity of Odorant-binding Proteins and Chemosensory Proteins in Insects

Cited by 206 publications

References 20 publications

An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas’ disease

An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas’ disease

RNAi-induced phenotypes suggest a novel role for a chemosensory protein CSP5 in the development of embryonic integument in the honeybee (Apis mellifera)

Genome-wide profiling of diel and circadian gene expression in the malaria vector Anopheles gambiae

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