Diversity of planktonic cyanobacteria and microcystin occurrence in Polish water bodies investigated using a polyphasic approach

Abstract

Microscopic measurements of fresh biomass and 16S rRNA gene sequences from clone libraries and denaturing gradient gel electrophoresis (DGGE) were used to investigate cyanobacterial diversity in Polish water bodies in 2002. In addition, measurements of microcystin (MC) concentrations were made. Thirty water samples were taken from 11 water bodies; of these samples, 18 were obtained from the Sulejow Reservoir during regular monitoring from June to October. Intraand extracellular MC concentrations in Sulejow samples were measured by high performance liquid chromatography (HPLC). The extracellular MC concentration was assessed using a protein phosphatase inhibition assay (PPIA) in additional lakes. Additionally, physicochemical parameters were measured (total nitrogen [TN], total phosphorus [TP], TN:TP ratio, chlorophyll a concentration, temperature). In Sulejow, high intracellular MC concentrations corresponded to large cyanobacterial biovolumes and to low TN:TP ratios. In the other lakes, extracellular MCs were not linked to any measured parameters. The combination of the microscopic and molecular data showed that Aphanizomenon and Microcystis were the dominant genera during the summer period in the Sulejow Reservoir. At the genetic level, there was a succession of 2 different operational taxonomic units (OTUs) belonging to the lineage Anabaena/Aphanizomenon. In the other water bodies, the most frequent populations were Aphanizomenon, Anabaena, Microcystis and Planktothrix. Small populations of Romeria, Snowella, Woronichinia, Limnothrix and Pseudanabaena were observed, and an enigmatic cluster affiliated with Prochlorothrix was genetically retrieved. Anabaena and Microcystis were presumed to be the main genera responsible for the MC production.

Cyanophages Infection of Microcystis Bloom in Lowland Dam Reservoir of Sulejów, Poland

“…Moreover, because of the specificity of the g91-gene, the present study focused exclusively on tracking the interactions between cyanophages from the family Myoviridae and their specific host, the Microcystis genus. Long-term studies have shown the predominance of the genus Microcystis, including M. aeruginosa species, in the Sulejów Reservoir in subsequent years [39,43,45,46]. Cyanophages belonging to the family Myoviridae accompanied cyanobacteria of the genus Microcystis in all surveyed seasons (2009)(2010)(2011)(2012)(2013).…”

Genetic diversity of symbiotic cyanobacteria in Cycas revoluta (Cycadaceae)

“…Primers 16S359F and 16S781R, reported by Nübel et al (1997), have been commonly used for specific amplification of the cyanobacterial 16S rDNA fragment (e.g. Boutte et al, 2006Boutte et al, , 2008. PCR was conducted in a total reaction volume of 20 lL containing 6.9 lL of autoclaved ion-exchanged water, 10 lL of Ampdirect Plus (Shimadzu, Kyoto, Japan), 0.1 lL of Ex Taq (Takara Bio, Ohtsu, Japan), 1.0 lL of each primer, and 1.0 lL of template DNA.…”

Zooplankton community structure in the presence of low levels of cyanotoxins: a case study in a high altitude tropical reservoir (Valle de Bravo, Mexico)

“…Among them are Microcystis and Anabaena which frequently dominate the phytoplankton community (Ramírez˗García et al, 2002;Gaytan˗Herrera et al, 2011). Snowella septentrionalis and Aphanizomenon yezoense were also present in this reservoir but these are often non-toxic (Boutte et al, 2008 . Cyanobacterial blooms are a common phenomenon in several water bodies in Mexico primarily due to the prevailing high nutrient and temperature regimes found in these habitats (De la Lanza and García, 2002;Berry et al, 2011;Kosten et al, 2012).…”