Diversity of plant heat shock factors: regulation, interactions, and functions

Andrási, Norbert; Pettkó‐Szandtner, Aladár; Szabados, László

doi:10.1093/jxb/eraa576

Cited by 131 publications

(124 citation statements)

References 180 publications

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“…Low temperature stress can increase the expression of six SAUR genes (PtSAUR12, PtSAUR34, PtSAUR54, PtSAUR67, PtSAUR91, and PtSAUR97) in poplar trees (Hu et al, 2018). HSF (Heat shock transcription factor) is an important factor in the regulation of plant stress resistance, and plays a key role in plant cold stress response, heat resistance, drought tolerance, and salt stress (Andrási et al, 2021). The overexpressed HbHsfA1 and HbHsfB1 may be candidates to improve cold stress tolerance of rubber trees (Deng et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

The Distribution and Origins of Pyrus hopeiensis-“Wild Plant With Tiny Population” Using Whole Genome Resequencing

Zhang

Wang

et al. 2021

Front. Plant Sci.

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Pyrus hopeiensis is a valuable but endangered wild resource in the genus Pyrus. It has been listed as one of the 120 wild species with tiny population in China. The specie has been little studied. A preliminary study of propagation modes in P. hopeiensis was performed through seed propagation, hybridization, self-crossing trials, bud grafting, branch grafting, and investigations of natural growth. The results showed that the population size of P. hopeiensis was very small, the distribution range was limited, and the habitat was extremely degraded. In the wild population, natural hybridization and root tiller production were the major modes of propagation. Whole genome re-sequencing of the 23 wild and cultivated accessions from Pyrus species collected was performed using an Illumina HiSeq sequencing platform. The sequencing depth range was 26.56x−44.85x and the average sequencing depth was 32x. Phylogenetic tree and principal component analyses (PCA) based on SNPs showed that the wild Pyrus species, such as PWH06, PWH07, PWH09, PWH10, PWH13, and PWH17, were closely related to both P. hopeiensis HB-1 and P. hopeiensis HB-2. Using these results in combination with morphological characteristics, it speculated that P. hopeiensis populations may form a natural hybrid group with frequent gene exchanges between and within groups. A selective elimination analysis on the P. hopeiensis population were performed using Fst and π radio and a total of 381 overlapping genes including SAUR72, IAA20, HSFA2, and RKP genes were obtained. These genes were analyzed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) function enrichment. And four KEGG pathways, including lysine degradation, sphingolipid metabolism, other glycan degradation, and betaine biosynthesis were significantly enriched in the P. hopeiensis population. Our study provided information on genetic variation, evolutionary relationships, and gene enrichment in P. hopeiensis population. These data will help reveal the evolutionary history and origin of P. hopeiensis and provide guidelines for subsequent research on the locations of functional genes.

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Section: Discussionmentioning

confidence: 99%

The Distribution and Origins of Pyrus hopeiensis-“Wild Plant With Tiny Population” Using Whole Genome Resequencing

Zhang

Wang

et al. 2021

Front. Plant Sci.

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“…HSFs have been reported to play a major role not only under stressful conditions but also in plant growth and development [16,72]. Therefore, the regulatory functions of maize HSFs were predicted through GO annotation investigation based on the biological process (BP), molecular function (MF), and cellular component (CC) classes (Figure 11; Table S6).…”

Section: Functional Annotation Of Maize Hsfsmentioning

confidence: 99%

“…Several TFs families including heat shock transcription factors (HSFs), WRKY (named due to conserved WRKYGQK motif), v-myb avian myeloblastosis viral oncogene homolog (MYB), Petunia NAM, Arabidopsis ATAF1/2 and CUC2 (NAC), and dehydration responsive-element binding transcriptional activator (DREB), etc., have been shown to positively regulate HS-responsive gene expression and improve plant HS tolerance [14,15]. Among them, the most comprehensively analyzed family is HSFs, the members of which are considered the master regulators of plant HS-response (HSR) and are also involved in regulating plant responses to other abiotic and biotic stress conditions [12,16]. Since the first HSF gene was identified in yeast [17], the HSF gene family has been characterized in several plant species [18,19].…”

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confidence: 99%

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Genome-wide Investigation and Expression Profiles of Heat Shock Transcription Factor (HSF) Gene Family in Maize (<em>Zea mays</em> L.) under Different Growth Stages and Abiotic Stress Conditions

Haider¹,

Rehman²,

Ahmad³

et al. 2021

Preprint

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Heat shock transcription factors (HSFs) participate in regulating many environmental stress responses and biological processes in plants. Maize (Zea mays L.) is a major cash crop that is grown worldwide. However, the growth and yield of maize are affected by several adverse environmental inputs. Therefore, investigating the factors that regulate maize growth and development and resistance to abiotic stress is an essential task for developing stress-resilient maize varieties. Thus, a comprehensive genome-wide identification analysis was performed to identify HSFs in the maize genome. The current study identified 25 ZmHSFs, randomly distributed throughout the maize genome. Phylogenetic analysis revealed that ZmHSFs are divided into three classes and 13 sub-classes. Gene structure and protein motif analysis supported the results obtained through the phylogenetic analysis. Domain analysis showed the DNA-binding domain to be the most conserved region of ZmHSFs. Segmental duplication is shown to be responsible for the expansion of ZmHSFs. Most of the ZmHSFs are localized inside the nucleus, and the ZmHSFs which belong to the same group show similar physio-chemical properties. The 3D structures revealed comparable conserved ZmHSFs protein structures. RNA-seq analysis revealed a major role of class A HSFs including, ZmHSFA-1a and ZmHSFA-2a in all the maize growth stages, i.e., seed, vegetative, and reproductive development. Furthermore, ZmHSFs displayed an obvious spatiotemporal expression. Under abiotic stress conditions (heat, drought, cold, UV, and salinity), members of class A and B ZmHSFs are induced. Gene ontology (GO) annotation analysis indicated a major role of ZmHSFs in resistance to environmental stress and regulation of primary metabolism. Further, the protein-protein interaction analysis showed that ZmHSFs interact with several molecular chaperons and major stress-responsive proteins. To summarize, this study provides novel insights for functional studies on the ZmHSFs in maize breeding programs.

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“…HSR involves a multitude of adaptive molecular processes, including activation of signalling components and transcription factors (Singh et al 2021). Heat shock transcription factors (Hsfs) serve as pivotal players in HSR by functioning as direct transcriptional regulators of heat shock proteins (Hsps), other stress‐related genes and transcription factors (Andrási et al 2021; Ding et al 2020; Ohama et al 2017). Most Hsps function as molecular chaperones in maintaining cellular protein homeostasis.…”

Section: Introductionmentioning

confidence: 99%

Hsp70, sHsps and ubiquitin proteins modulate HsfA6a‐mediated Hsp101 transcript expression in rice (Oryza sativa L.)

Singh

Sarkar

Grover

2021

Physiologia Plantarum

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Hsp100 chaperones disaggregate the aggregated proteins and are vital for maintenance of protein homeostasis. The level of Hsp100 synthesised in the cells has a bearing on the survival of plants under heat stress. The Hsp100 transcription machinery is activated within minutes of the onset of heat stress. The heat shock factor HsfA6a plays a major role in the transcriptional regulation of the Hsp101 gene in rice plants. Through yeast‐2‐hybrid library screening, we identified small heat shock proteins (sHSPs), Hsp70 and ubiquitin as HsfA6a interacting proteins (HIPs). The bimolecular fluorescence complementation assays showed the physical interaction of HsfA6a with Hsp16.9A‐CI and Hsp18.0‐CII in the cytosolic region and with cHsp70‐1 in the nucleus. With the Hsp101 promoter: reporter gene assays, using yeast cells and rice protoplasts, we show that CI‐sHsps and CII‐sHsps are negative regulators and Hsp70 positive regulator of the HsfA6a activity in modulation of Hsp101 transcription. We also noted that the HsfA6a interactors, Hsp70 and CI‐sHsps and CII‐sHsps, physically interact with each other. We noted that HsfA6a binds with the CI‐sHsp and Hsp70 promoters, implying that HsfA6a has a role in transcriptional regulation of its interacting proteins. Furthermore, we noted that the mutation of the ubiquitin/sumoylation acceptor site lysine 10 to alanine (K10A) of HsfA6a enhanced its DNA binding potential on the Hsp101 promoter, implying that these modifiers are possibly involved in modulation of HsfA6a activity. Our work shows that Hsp70, CI‐sHsps and CII‐sHsp, and ubiquitin proteins coordinate with HsfA6a in mediating the Hsp101 transcription process in rice.

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Diversity of plant heat shock factors: regulation, interactions, and functions

Cited by 131 publications

References 180 publications

The Distribution and Origins of Pyrus hopeiensis-“Wild Plant With Tiny Population” Using Whole Genome Resequencing

The Distribution and Origins of Pyrus hopeiensis-“Wild Plant With Tiny Population” Using Whole Genome Resequencing

Genome-wide Investigation and Expression Profiles of Heat Shock Transcription Factor (HSF) Gene Family in Maize (<em>Zea mays</em> L.) under Different Growth Stages and Abiotic Stress Conditions

Hsp70, sHsps and ubiquitin proteins modulate HsfA6a‐mediated Hsp101 transcript expression in rice (Oryza sativa L.)

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