Diversity of site-specific microbes of occlusal and proximal lesions in severe- early childhood caries (S-ECC)

Abstract

Background Severe-early childhood caries (S-ECC) a global problem of significant concern, commonly manifest on the occlusal, and proximal surfaces of affected teeth. Despite the major ecological differences between these two niches the compositional differences, if any, in the microbiota of such lesions is unknown. Methods Deep-dentine caries samples from asymptomatic primary molars of children with S-ECC (n 19) belonging to caries-code 5/6, (ICDAS classification) were evaluated. Employing two primer pools, we amplified and compared the bacterial 16S rRNA gene sequences of the seven hypervariable regions (V2—V4 and V6—V9) using NGS-based assay. Results Bray-Curtisevaluation indicated that occlusal lesions (OL) had a more homogeneous community than the proximal lesions (PL) with significant compositional differences at the species level ( p = 0.01; R- 0.513). Together, the occlusal and proximal niches harbored 263 species, of which 202 (76.8%) species were common to both , while 49 (18.6%) and 12 (4.6%) disparate species were exclusively isolated from the proximal and occlusal niches, respectively. The most commonl genera at both niches included Streptococcus, Prevotella , and Lactobacillus. S. mutans was predominant in PL ( p ≤ 0.05), and Atopobium parvulum ( p = 0.01) was predominant in OL. Conclusions Distinct differences exist between the caries microbiota of occlusal and proximal caries in S-ECC.

Role of socioeconomic factors and interkingdom crosstalk in the dental plaque microbiome in early childhood caries

“…Chen et al, 2020). From our results, N. oralis can be considered a potential biomarker for CF status, while the role of N. bacilliformis in ECC has been further emphasized by recent studies (Cherkasov et al, 2019;Fakhruddin et al, 2022;E. Lee et al, 2021).…”

Using Nanopore Sequencing to Obtain Complete Bacterial Genomes from Saliva Samples

“…7 ). In the oral cavity, L. parvulum has been associated with occlusal lesions in caries ( 48 ), or with a healthy microbiome, in the context of periodontal disease ( 49 ). In the gut, L. parvulum has been associated with colorectal cancer ( 50 ) and the onset of Crohn’s disease, due to its ability to produce H2S through the SufS cysteine desulfurase ( 51 , 52 ).…”

Using nanopore sequencing to obtain complete genomes from saliva samples

“…Compared to IPP1246, JCVI-JB-Lp32 had 178 unique gene clusters, and was missing 50 gene clusters uniquely compared to all other Atopobiaceae analyzed (Figure 7). In the oral cavity, L. parvulum has been associated with occlusal lesions in caries (Fakhruddin, Samaranayake, Hamoudi, Ngo, & Egusa, 2022), or with a healthy microbiome, in the context of periodontal disease (Kumar et al, 2003). In the gut, L. parvulum has been associated with colorectal cancer (Yachida et al, 2019) and the onset of Crohn’s disease, due to its ability to produce H2S through the SufS cysteine desulfurase (Karunakaran et al, 2022; Mottawea et al, 2016).…”

“…Atopobiaceae analyzed (Figure 7). In the oral cavity, L. parvulum has been associated with occlusal lesions in caries (Fakhruddin, Samaranayake, Hamoudi, Ngo, & Egusa, 2022), or with a healthy microbiome, in the context of periodontal disease (Kumar et al, 2003). In the gut, L.…”