Diversity of teleost leukocyte molecules: Role of alternative splicing

Maisey, Kevin; Imarai, Mónica

doi:10.1016/j.fsi.2010.08.001

Cited by 11 publications

(4 citation statements)

References 123 publications

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“…In catfish, the alternative splicing of IL-8-like genes [ 16 ] resulted in normal and alternatively spliced forms being expressed at different levels. This suggests that alternative splicing is an important mechanism for modulating gene expression and function of fish IL-8 genes [ 50 , 58 ], similar to the CXC genes in Nile tilapia. However, the expressions of both Nile tilapia CXC chemokine genes in this study were decreased to baseline levels at day 2.…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification and Dual Functions of Two Different CXC Chemokines in Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae and Flavobacterium columnare

Nakharuthai

Srisapoome

2020

Microorganisms

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Two CXC chemokines in Nile tilapia (On-CXC1 and On-CXC2) were identified at both the genomic and proteomic levels. A southern blot analysis and comparison searching in Ensembl confirmed the typical structure of the CXC chemokine genes and provided evidence for unusual mechanisms used to generate the two different CXC chemokine transcripts that have not been reported in other vertebrate species so far. The expression levels of On-CXC1 and On-CXC2 were analyzed by quantitative real-time PCR. These two mRNAs were detected in various tissues of normal Nile tilapia, especially in the spleen, heart, and head kidney, indicating a homeostatic function in immunosurveillance. A time-course experiment clearly demonstrated that these two transcripts were effectively enhanced in the head kidney, spleen and trunk kidney of Nile tilapia 6, 12 and 24 h after injection with Streptococcus agalactiae but were down-regulated in all tested tissues at 48 h, reflecting the fact that they have short half-lives during the crucial response to pathogens that is characteristic of CXC chemokine genes in other vertebrates. Functional analyses obviously exhibited that these two CXC chemokines at concentrations of 1–10 μg strongly inactivated S. agalactiae and Flavobacterium columnare and effectively induced phagocytosis of leukocytes in vitro.

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Section: Discussionmentioning

confidence: 99%

Molecular Identification and Dual Functions of Two Different CXC Chemokines in Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae and Flavobacterium columnare

Nakharuthai

Srisapoome

2020

Microorganisms

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“…Alternative splicing, which can lead to the loss of an Ig domain (e.g. dicp2.1 and dicp3.1), likely contributes to the complexity of the immune receptor repertoire (Maisey and Imarai 2011).…”

Section: Discussionmentioning

confidence: 99%

The identification of additional zebrafish DICP genes reveals haplotype variation and linkage to MHC class I genes

et al. 2016

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Bony fish encode multiple multi-gene families of membrane receptors that are comprised of immunoglobulin (Ig) domains and are predicted to function in innate immunity. One of these families, the diverse immunoglobulin (Ig) domain-containing protein (DICP) genes, maps to three chromosomal loci in zebrafish. Most DICPs possess one or two Ig ectodomains and include membrane-bound and secreted forms. Membrane-bound DICPs include putative inhibitory and activating receptors. Recombinant DICP Ig domains bind lipids with varying specificity, a characteristic shared with mammalian CD300 and TREM family members. Numerous DICP transcripts amplified from different lines of zebrafish did not match the zebrafish reference genome sequence suggesting polymorphic and haplotypic variation. The expression of DICPs in three different lines of zebrafish has been characterized employing PCR-based strategies. Certain DICPs exhibit restricted expression in adult tissues whereas others are expressed ubiquitously. Transcripts of a subset of DICPs can be detected during embryonic development suggesting roles in embryonic immunity or other developmental processes. Transcripts representing 11 previously uncharacterized DICP sequences were identified. The assignment of two of these sequences to an unplaced genomic scaffold resulted in the identification of an alternative DICP haplotype that is linked to a MHC class I Z lineage haplotype on zebrafish chromosome 3. The linkage of DICP and MHC class I genes also is observable in the genomes of the related grass carp (Ctenopharyngodon idellus) and common carp (Cyprinus carpio) suggesting that this is a shared character with the last common Cyprinidae ancestor.

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“…Further, IgT/Z shares many functional similarities with mammalian IgA [22]. Even if IgT/IgZ cannot serve as IgA equivalent in teleosts, we cannot neglect the "power" of alternative splicing of pre-mRNA in fish, recently summarized by Maisey and Imarai [48] and Quiniou et al [49]. Such splicing may also be responsible for differences in IgM heavy chains that can result in mucosal and systemic IgM variants [22].…”

Section: Mucosal Antibodiesmentioning

confidence: 96%

Adaptive immune responses at mucosal surfaces of teleost fish

Rombout

Gao

Kiron

2014

Fish & Shellfish Immunology

258

141

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This review describes the extant knowledge on the teleostean mucosal adaptive immune mechanisms, which is relevant for the development of oral or mucosal vaccines. In the last decade, a number of studies have shed light on the presence of new key components of mucosal immunity: a distinct immunoglobulin class (IgT or IgZ) and the polymeric Ig receptor (pIgR). In addition, intestinal T cells and their putative functions, antigen uptake mechanisms at mucosal surfaces and new mucosal vaccination strategies have been reported. New information on pIgR of Atlantic cod and common carp and comparison of natural and specific cell-mediated cytotoxicity in the gut of common carp and European seabass, is also included in this review. Based on the known facts about intestinal immunology and mucosal vaccination, suggestions are made for the advancement of fish vaccines.

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Diversity of teleost leukocyte molecules: Role of alternative splicing

Cited by 11 publications

References 123 publications

Molecular Identification and Dual Functions of Two Different CXC Chemokines in Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae and Flavobacterium columnare

Molecular Identification and Dual Functions of Two Different CXC Chemokines in Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae and Flavobacterium columnare

The identification of additional zebrafish DICP genes reveals haplotype variation and linkage to MHC class I genes

Adaptive immune responses at mucosal surfaces of teleost fish

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