Keywords: conservation biology, endangered weed, genetic variation, random amplified polymorphic DNA, Ranunculus kadzusensis, rice field. barium specimens in South Korea; therefore, Lee (1990) presumed it to be extinct.There is increasing interest in endangered plants that were common in the past but are rare now in arable land. Recent social demand to conserve endangered plants in arable land requires knowledge of the biological characteristics of each species. However, surprisingly little is known about the present distribution and population's genetic structure of R. kadzusensis. The purpose of the present study was to accumulate information on the distribution and habitat of R. kadzusensis (or R. trichophyllus) and to measure the genetic variation with random amplified polymorphic DNA (RAPD) analysis for its conservation.
METHODS
Field surveyThe collection sites of R. kadzusensis or R. trichophyllus that were reported in the database of plant specimens in 29 herbaria of South Korea (in Korean with a photograph of the specimen) (Korea Forest Service 2001) were surveyed from 2001 to 2002 to confirm its present distribution. Based on this survey, 11 populations at eight regions were selected for further genetic analysis (Fig. 1). Twelve plant samples were collected in the populations in 2003, but in Haechang population, only eight samples were collected due to the difficulty of sample collection in a submerged state. One individual was collected in one field lot. Several individuals were sampled in Dongmak, Baetmal, Haechang, and Yongdae, where the populations consisted of < 12 field lots.