Diversity of total, nitrogen‐fixing and denitrifying bacteria in an acid forest soil

Rösch, Christopher; Bothe, Hermann

doi:10.1111/j.1365-2389.2009.01167.x

Cited by 24 publications

(11 citation statements)

References 77 publications

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“…The CF amendments significantly decreased the soil pH, which was a negative factor regarding the copy numbers of denitrifying genes and could counteract the positive effects of higher SOC induced by CFs (Hallin et al, 2009). In addition, the effect of CFs on denitrifier abundance is likely to be related to the soil C:N ratio, which was reduced by the N fertilizer amendments, changing the population of denitrifiers (Rösch and Bothe, 2009).…”

Section: Temporal Changes In the Abundance Of Denitrifiers Under Diffmentioning

confidence: 99%

不同肥料处理下茶园土壤细菌和古菌群落的时间变化研究

Wang

Yang

et al. 2014

J. Zhejiang Univ. Sci. B

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Abstract:It is important to understand the effects of temporal changes in microbial communities in the acidic soils of tea orchards with different fertilizers. A field experiment involving organic fertilizer (OF), chemical fertilizer (CF), and unfertilized control (CK) treatments was arranged to analyze the temporal changes in the bacterial and archaeal communities at bimonthly intervals based on the 16S ribosomal RNA (rRNA) gene using terminal restriction fragment length polymorphism (T-RFLP) profiling. The abundances of total bacteria, total archaea, and selected functional genes (bacterial and archaeal amoA, bacterial narG, nirK, nirS, and nosZ) were determined by quantitative polymerase chain reaction (qPCR). The results indicate that the structures of bacterial and archaeal communities varied significantly with time and fertilization based on changes in the relative abundance of dominant T-RFs. The abundancy of the detected genes changed with time. The total bacteria, total archaea, and archaeal amoA were less abundant in July. The bacterial amoA and denitrifying genes were less abundant in September, except the nirK gene. The OF treatment increased the abundance of the observed genes, while the CF treatment had little influence on them. The soil temperature significantly affected the bacterial and archaeal community structures. The soil moisture was significantly correlated with the abundance of denitrifying genes. Of the soil chemical properties, soil organic carbon was the most important factor and was significantly correlated with the abundance of the detected genes, except the nirK gene. Overall, this study demonstrated the effects of both temporal alteration and organic fertilizer on the structures of microbial communities and the abundance of genes involved in the nitrogen cycle.

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Section: Temporal Changes In the Abundance Of Denitrifiers Under Diffmentioning

confidence: 99%

不同肥料处理下茶园土壤细菌和古菌群落的时间变化研究

Wang

Yang

et al. 2014

J. Zhejiang Univ. Sci. B

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“…In addition, as actinobacteria are important organic matter decomposers, rich actinobacterial communities can be expected in ecosystems that vary regarding the quantity and quality of organic matter. Since biodiversity is regarded as proportional to the number of available niches (Hutchinson, 1961;Torsvik et al, 2002;Rosch & Bothe, 2009) and soil is generally a highly structured environment (Ranjard & Richaume, 2001), we inferred that the exploration of sites with variable environmental factors may reveal new actinobacteria diversity (Lazzirini et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Actinobacterial community dominated by a distinct clade in acidic soil of a waterlogged deciduous forest

Kopecký

Kyselková

Omelka

et al. 2011

FEMS Microbiology Ecology

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Members of the Actinobacteria are among the most important litter decomposers in soil. The site of a waterlogged deciduous forest with acidic soil was explored for actinobacteria because seasonality of litter inputs, temperature, and precipitation provided contrasting environmental conditions, particularly variation of organic matter quantity and quality. We hypothesized that these factors, which are known to influence decomposition, were also likely to affect actinobacterial community composition. The relationship between the actinobacterial community, soil moisture and organic matter content was assessed in two soil horizons in the summer and winter seasons using a 16S rRNA taxonomic microarray and cloning-sequencing of 16S rRNA genes. Both approaches showed that the community differed significantly between horizons and seasons, paralleling the changes in soil moisture and organic matter content. The microarray analysis further indicated that the actinobacterial community of the upper horizon was characterized by high incidence of the genus Mycobacterium. In both horizons and seasons, the actinobacterial clone libraries were dominated (by 80%) by sequences of a separate clade sharing an ancestral node with Streptosporangineae. This relatedness is supported also by some common adaptations, for example, to soil acidity and periodic oxygen deprivation or dryness.

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“…As mentioned above, nifH is very much conserved during evolution, and probing with nifH sequences should allow one to detect all N 2 -fixing microorganisms in environmental samples. Recent studies showed that most of the bacterial DNA sequences from soil (for nifH as well as for nosZ in denitrification and for the 16S rRNA gene for total bacteria) could be detected with the short DNA probes available, but the gene sequences in total were entirely new (60,180).…”

Section: Nitrogen Fixation In Nonheterocystous Cyanobacteriamentioning

confidence: 99%

Nitrogen Fixation and Hydrogen Metabolism in Cyanobacteria

Bothe

Schmitz²,

Yates

et al. 2010

Microbiol Mol Biol Rev

365

222

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SUMMARY This review summarizes recent aspects of (di)nitrogen fixation and (di)hydrogen metabolism, with emphasis on cyanobacteria. These organisms possess several types of the enzyme complexes catalyzing N2 fixation and/or H2 formation or oxidation, namely, two Mo nitrogenases, a V nitrogenase, and two hydrogenases. The two cyanobacterial Ni hydrogenases are differentiated as either uptake or bidirectional hydrogenases. The different forms of both the nitrogenases and hydrogenases are encoded by different sets of genes, and their organization on the chromosome can vary from one cyanobacterium to another. Factors regulating the expression of these genes are emerging from recent studies. New ideas on the potential physiological and ecological roles of nitrogenases and hydrogenases are presented. There is a renewed interest in exploiting cyanobacteria in solar energy conversion programs to generate H2 as a source of combustible energy. To enhance the rates of H2 production, the emphasis perhaps needs not to be on more efficient hydrogenases and nitrogenases or on the transfer of foreign enzymes into cyanobacteria. A likely better strategy is to exploit the use of radiant solar energy by the photosynthetic electron transport system to enhance the rates of H2 formation and so improve the chances of utilizing cyanobacteria as a source for the generation of clean energy.

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Diversity of total, nitrogen‐fixing and denitrifying bacteria in an acid forest soil

Cited by 24 publications

References 77 publications

不同肥料处理下茶园土壤细菌和古菌群落的时间变化研究

不同肥料处理下茶园土壤细菌和古菌群落的时间变化研究

Actinobacterial community dominated by a distinct clade in acidic soil of a waterlogged deciduous forest

Nitrogen Fixation and Hydrogen Metabolism in Cyanobacteria

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