Diversity of Trypanosomatids in Cockroaches and the Description of<i>Herpetomonas tarakana</i>sp. n.

Yurchenko, Vyacheslav; Kostygov, Alexei Y.; Havlová, J.; Grybchuk-Ieremenko, Anastasiia; Ševčíková, Tereza; Lukeš, Julius; Ševčı́k, Jan; Votýpka, Jan

doi:10.1111/jeu.12268

Cited by 47 publications

(43 citation statements)

References 51 publications

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“…The genus Phytomonas was polyphyletic in our analyses, differing from previous studies in which its monophyly was recovered based on molecular data (e.g. Hughes and Piontkivska, ; Teixeira et al., ; Votýpka et al., , ; Yurchenko et al., , ; Jaskowska et al., ; Zanetti et al., ). Although such a result may relate to discrepancies in taxon sampling, the affinity of Phytomonas to Herpetomonas , and the closer affinity of Phytomonas macgheei to P. serpens than to Phytomonas francai , partially corroborate the literature (e.g.…”

Section: Discussioncontrasting

confidence: 99%

Evaluating the role of morphological characters in the phylogeny of some trypanosomatid genera (Excavata, Kinetoplastea, Trypanosomatida)

Silva-Júnior

Paiva

2017

Cladistics

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Over the last three decades, it has been progressively assumed that morphology has become obsolete for trypanosomatid systematics. Traditional taxonomy, based on the occurrence of specific kinds of cell morphotypes during life cycles and the morphometry of such cells, is often rejected by molecular phylogenies inferred mostly from 18S rDNA alone or combined with GAPDH. In such context, we hypothesized the affinities of 35 representatives of seven trypanosomatid genera from separated and combined cladistics analyses of morphological and 18S matrices. Morphology is shown to be more consistent and to have stronger synapomorphy retention than the 18S data. The strict consensus of cladograms from separated analyses was mostly unresolved, while combined analysis produced a meaningful and robust phylogenetic pattern, as evidenced by partition congruence index, Bremer support and frequencies of groups present/contradicted. The results (1) corroborate the separation of Angomonas and Strigomonas from Crithidia, which is now shown to be monophyletic, (2) support the revalidation of the genus Wallaceina, and (3) place the genera Herpetomonas, Leptomonas and Phytomonas within a single clade. Overall, we demonstrate the belief that morphological characters are inferior to molecular ones for trypanosomatid systematics is a consequence of neglecting their inclusion in phylogenetic analyses.

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Section: Discussioncontrasting

confidence: 99%

Evaluating the role of morphological characters in the phylogeny of some trypanosomatid genera (Excavata, Kinetoplastea, Trypanosomatida)

Silva-Júnior

Paiva

2017

Cladistics

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“…The initial taxonomic identification was provided by the original depositors. Trypanosomatids were grown at 23°C in liquid Brain Heart Infusion (BHI) medium (Sigma-Aldrich, St. Louis, USA) supplemented as described previously [41] and passaged weekly. For growth curves at different temperatures (23°C and 34°C), flagellates were seeded at a concentration of 30,000 cells per ml, and counted in triplicates at days 1, 3, and 5.…”

Section: Methodsmentioning

confidence: 99%

Molecular mechanisms of thermal resistance of the insect trypanosomatid Crithidia thermophila

et al. 2017

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In the present work, we investigated molecular mechanisms governing thermal resistance of a monoxenous trypanosomatid Crithidia luciliae thermophila, which we reclassified as a separate species C. thermophila. We analyzed morphology, growth kinetics, and transcriptomic profiles of flagellates cultivated at low (23°C) and elevated (34°C) temperature. When maintained at high temperature, they grew significantly faster, became shorter, with genes involved in sugar metabolism and mitochondrial stress protection significantly upregulated. Comparison with another thermoresistant monoxenous trypanosomatid, Leptomonas seymouri, revealed dramatic differences in transcription profiles of the two species with only few genes showing the same expression pattern. This disparity illustrates differences in the biology of these two parasites and distinct mechanisms of their thermotolerance, a prerequisite for living in warm-blooded vertebrates.

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“…Another genome, recently sequenced and annotated by us, is that of a trypanosomatid parasitizing fleas, Blechomonas ayalai, which belong to an early-branching clade Blechomonadinae, a sister group to subfamilies Phytomonadinae and Leishmaniinae (Fig. 1) (Jirk u et al 2012;Yurchenko et al 2016).…”

mentioning

confidence: 99%

Comparative Metabolism of Free‐living Bodo saltans and Parasitic Trypanosomatids

Opperdoes

Butenko

Flegontov

et al. 2016

J Eukaryotic Microbiology

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Comparison of the genomes of free-living Bodo saltans and those of parasitic trypanosomatids reveals that the transition from a free-living to a parasitic life style has resulted in the loss of approximately 50% of protein-coding genes. Despite this dramatic reduction in genome size, B. saltans and trypanosomatids still share a significant number of common metabolic traits: glycosomes; a unique set of the pyrimidine biosynthetic pathway genes; an ATP-PFK which is homologous to the bacterial PPi -PFKs rather than to the canonical eukaryotic ATP-PFKs; an alternative oxidase; three phosphoglycerate kinases and two GAPDH isoenzymes; a pyruvate kinase regulated by fructose-2,6-bisphosphate; trypanothione as a substitute for glutathione; synthesis of fatty acids via a unique set of elongase enzymes; and a mitochondrial acetate:succinate coenzyme A transferase. B. saltans has lost the capacity to synthesize ubiquinone. Among genes that are present in B. saltans and lost in all trypanosomatids are those involved in the degradation of mureine, tryptophan and lysine. Novel acquisitions of trypanosomatids are components of pentose sugar metabolism, pteridine reductase and bromodomain-factor proteins. In addition, only the subfamily Leishmaniinae has acquired a gene for catalase and the capacity to convert diaminopimelic acid to lysine.

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Diversity of Trypanosomatids in Cockroaches and the Description ofHerpetomonas tarakanasp. n.

Cited by 47 publications

References 51 publications

Evaluating the role of morphological characters in the phylogeny of some trypanosomatid genera (Excavata, Kinetoplastea, Trypanosomatida)

Evaluating the role of morphological characters in the phylogeny of some trypanosomatid genera (Excavata, Kinetoplastea, Trypanosomatida)

Molecular mechanisms of thermal resistance of the insect trypanosomatid Crithidia thermophila

Comparative Metabolism of Free‐living Bodo saltans and Parasitic Trypanosomatids

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