Diversity of Vibrio parahaemolyticus associated with disease outbreak among cultured Litopenaeus vannamei (Pacific white shrimp) in India

Kumar, Ballamoole Krishna; Deekshit, Vijaya Kumar; Raj, Juliet Roshini Mohan; Rai, Praveen; Shivanagowda, Balligavi Mallappa; Karunasagar, Iddya; Karunasagar, Indrani

doi:10.1016/j.aquaculture.2014.06.016

Cited by 61 publications

(26 citation statements)

References 33 publications

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“…The symptoms of LSSDaffected individuals were similar to the previous reports of LSSD in tiger shrimp from India (Lightner & Redman 1998;Gopalakrishnan & Parida 2005;Alavandi et al 2008;Kalaimani et al 2013). The predominant Vibrio species found in the cultured shrimps have developed as the active pathogen when the immune system of the shrimps is suppressed (Brock & Lightner 1990;Kumar et al 2014). The optimum temperature for the grow-out ponds ranges from 25°C to 28°C.…”

Section: Discussionmentioning

confidence: 99%

First incidence of loose-shell syndrome disease in the giant tiger shrimp Penaeus monodon from the brackish water ponds in Bangladesh

Aftabuddin

Roman

Hasan

et al. 2017

Journal of Applied Animal Research

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The recent incidence of loose-shell syndrome disease (LSSD) in grow-out shrimp ponds appears to be a major problem in Bangladesh. Therefore, the aims of our study were (i) to observe clinical signs of LSSD to confirm its incidence and (ii) to identify the probable causative agents for LSSD in shrimp. Sampling was conducted randomly from five LSSD-affected ponds and three non-affected ponds near Bakkhali River; ecological parameters were measured. A total of 180 healthy shrimps were used for this experiment, where LSSD-affected shrimp extracts were either injected into the shrimps or mixed with water in the experimental tanks. Finally, microbial examinations were performed to identify the possible LSSD causative agents from the infected individuals. The total shrimp production was higher in ponds with healthy populations (185 kg/ha) than from LSSD-affected ponds (126-146 kg/ha); the survival rate of shrimp at harvest was significantly different (p < .01) between normal and LSSDaffected ponds. The prevalence of infection with white spot syndrome virus (WSSV) and monodon baculovirus (MBV) was found to be lower than that of the Vibrio infections. During the investigation, 8% of LSSD-affected individuals were WSSV positive and 5% were MBV positive, and 4% were infected by both. Our study suggested that the prevalence of LSSD in tiger shrimp might be associated with multiple Vibrio bacterial infections, poor soil and water quality, as well as poor pond management. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

First incidence of loose-shell syndrome disease in the giant tiger shrimp Penaeus monodon from the brackish water ponds in Bangladesh

Aftabuddin

Roman

Hasan

et al. 2017

Journal of Applied Animal Research

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“…In aquaculture, especially in fish and shrimp farming, the expansion of production areas and intensification of culture systems have led to the emergence of various bacterial, parasitic and viral diseases throughout the world (Kumar et al, 2014;Mo et al, 2015;Hai, 2015;Raja e Jithendran, 2015). To combat the spread of disease, antibiotics are typically applied.…”

Section: Introductionmentioning

confidence: 99%

Selection of autochtone probiotic for Astyanax bimaculatus

Jatobá

Moraes

Steckert

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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This study aimed to isolate native lactic acid bacteria of yellow tail lambari (Astyanax bimaculatus) and evaluate their effect on host microbiota and gut morphology, as well as survival after experimental challenge. The isolated bacterial strains were evaluated for their inhibition against pathogenic bacterial strains in vitro, and the strain with highest inhibitory ability was molecularly identified as Lactobacillus spp. For in vivo testing, eighty fish were distributed in ten tanks equipped with a recirculation system. The experimental units were divided into two treatments: fish fed with Lactobacillus spp. supplement and fish fed an unsupplemented diet (control). After 30 days, guts from three fish from each experimental unit were pooled for microbiological and histological analysis. The other five fish were inoculated with 2.1x10 4 CFU.mL -1 of Aeromonas hydrophila to evaluate survival after 24h. Lambaris fed with the probiotic diet had a lower count of Vibrios spp., Pseudomonas spp. and Staphylococcus spp., and a higher count of lactic acid bacteria compared to control treatment, as well as, increased length, width and perimeter of intestinal villi, as well as higher survival rate (16.2%) after experimental challenge compared to the unsupplemented group. The results show that the Lactobacillus spp. used has effect probiotic for yellow tail lambari.Keywords: Lactobacillus, yellow tail lambari, gut morphology, microbiota, experimental challenge RESUMO Este estudo objetivou isolar bactéria ácido-láctica nativa do lambari-do-rabo-amarelo (Astyanax bimaculatus) e seu efeito na microbiota e morfologia do trato digestório do hospedeiro, assim como a sobrevivência após um desafio experimental. As bactérias isoladas foram avaliadas quanto a suas inibições in vitro

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“…Therefore, the dLAMP-LFD method may not be suitable for application in a food safety perspective due to a limitation in quantitative capability. However, the dLAMP-LFD assay can be applied for an animal health aspect since V. vulnificus and V. parahaemolyticus are both potentially detrimental to shrimp culture (Oliver and Karper 2005;Kumar et al 2014;Yingkajorn et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Development of Duplex Loop‐Mediated Isothermal Amplification (dLAMP) Combined with Lateral Flow Dipstick (LFD) for the Rapid and Specific Detection of Vibrio vulnificus and V. parahaemolyticus

et al. 2016

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Loop-mediated isothermal amplification (LAMP) is a rapid, specific, and effective DNA amplification assay. In this study, a duplex LAMP (dLAMP) assay combined with chromatographic lateral flow dipstick (LFD) was developed for the simultaneous identification of Vibrio vulnificus and V. parahaemolyticus. Each primer set that comprised F3, B3, FIP, and BIP recognized the V. vulnificus rpoS gene and the V. parahaemolyticus tlh gene. Digoxigenilated and biotinylated LAMP amplicons of V. vulnificus and V. parahaemolyticus, respectively, were hybridized with corresponded fluorescein isothiocyanate (FITC)-labeled probes for LFD detection. The optimum conditions were 90 min at 63°C. The dLAMP-LFD had high specificity to V. vulnificus and V. parahaemolyticus and showed no cross-amplification with 59 isolates of other bacteria. The detection limit of dLAMP-LFD with a pure culture of V. vulnificus was 2.2 × 10 4 CFU/mL, corresponding to 41 CFU per reaction, which was equal to that of duplex PCR (dPCR). In the case of V. parahaemolyticus, the detection limit of dLAMP-LFD with a pure culture was 2.2 × 10 3 CFU/mL, corresponding to 4.1 CFU per reaction, 100 times more sensitive than that of dPCR. In the spiked shrimp samples, the detection limit of dLAMP-LFD for V. vulnificus was 2.2 × 10 5 CFU/g, corresponding to 410 CFU per reaction, 10 times more sensitive than that of dPCR. In the case of V. parahaemolyticus, the detection limit of dLAMP-LFD in spiked shrimp samples was 2.2 × 10 4 CFU/g, corresponding to 41 CFU per reaction, 100 times more sensitive than that of dPCR. These results demonstrate that the simple dLAMP-LFD assay had high specificity and high sensitivity for the concurrent detection of V. vulnificus and V. parahaemolyticus.

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Diversity of Vibrio parahaemolyticus associated with disease outbreak among cultured Litopenaeus vannamei (Pacific white shrimp) in India

Cited by 61 publications

References 33 publications

First incidence of loose-shell syndrome disease in the giant tiger shrimp Penaeus monodon from the brackish water ponds in Bangladesh

First incidence of loose-shell syndrome disease in the giant tiger shrimp Penaeus monodon from the brackish water ponds in Bangladesh

Selection of autochtone probiotic for Astyanax bimaculatus

Development of Duplex Loop‐Mediated Isothermal Amplification (dLAMP) Combined with Lateral Flow Dipstick (LFD) for the Rapid and Specific Detection of Vibrio vulnificus and V. parahaemolyticus

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