Diversity of Vibrios associated with reared clams in Galicia (NW Spain)

Hidalgo, Roxana Beaz; Cleenwerck, Ilse; Balboa, Sabela; Wachter, Marjan De; Thompson, Fabiano L.; Swings, Jean; Vos, Paul De; Romalde, Jesús L.

doi:10.1016/j.syapm.2008.04.001

Cited by 49 publications

(38 citation statements)

References 28 publications

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“…To date, the identification of Vibrio spp. isolated from aquaculture systems has been imprecise, and the taxonomy of Vibrio remains under revision (Vandenberghe et al 2003, Beaz-Hidalgo et al 2008, Noguerola & Blanch 2008. V. harveyi, which can be a primary pathogen in marine vertebrates and invertebrates (Austin & Zhang 2006), was the most prevalent clade, representing up to 52% of the total sequenced bacteria, in accordance with previous publications (Arias et al 1999, Castro et al 2002, Alves et al 2010.…”

Section: Discussionsupporting

confidence: 81%

Occurrence, seasonality and infectivity of Vibrio strains in natural populations of mussels Mytilus galloprovincialis

Romero

Costa²,

Forn-Cuní³

et al. 2014

Dis. Aquat. Org.

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Widespread and large-scale mortalities of bivalve molluscs significantly affect their production. A number of pathogens have been identified as the primary causes of death in oysters or clams, especially bacteria of the genus Vibrio. We evaluated the occurrence, seasonality and infectivity of Vibrio strains associated with natural mussel (Mytilus galloprovincialis) populations. In particular, different isolates of V. splendidus and V. aestuarianus were analysed because they were associated with major oyster mortalities in areas where mussels are cultured without presenting mortalities. The presence of both Vibrio spp. was analysed bimonthly in mussels, water, sediment, plankton and other associated fauna from 2 sites in Galicia (NW Spain), the region with the highest mussel production in Europe. Environmental factors were also considered. The pathogenicity of different Vibrio isolates was analysed by performing experimental infections in mussels with strains isolated from the field. Results showed that Vibrio populations were mainly influenced by changes in water temperature and salinity. V. splendidus was dominant during the warm months and V. aestuarianus was predominant throughout the cold season. The sediment was the most important natural reservoir for bacteria. Experimental infections showed the extreme resistance of mussels to bacterial pathogens. Isolates of V. splendidus and V. aestuarianus were only moderately pathogenic for mussels in intramuscular infections and bath infections, and mortalities only occurred when animals were infected with a high bacterial concentration in adverse environmental conditions (hypoxia and 25°C). Although the pathogenicity of the Vibrio strains isolated from the wild was low for mussels, their potential risk for other bivalves cannot be ignored.

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Section: Discussionsupporting

confidence: 81%

Occurrence, seasonality and infectivity of Vibrio strains in natural populations of mussels Mytilus galloprovincialis

Romero

Costa²,

Forn-Cuní³

et al. 2014

Dis. Aquat. Org.

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“…From 1990s the diversity of Vibrio species associated with bivalves in different geographical areas has been the subject of various studies (Montilla et al, 1994; Hariharan et al, 1995; Arias et al, 1999; Pujalte et al, 1999; Maugeri et al, 2000; Caballo and Stabili, 2002; Castro et al, 2002; Guisande et al, 2004; Beaz-Hidalgo et al, 2008, 2010a; Lafisca et al, 2008). The main common conclusions obtained from these studies were that environmental parameters, such as variations in the water temperature and salinity, can influence the diversity of Vibrio spp.…”

Section: Vibrios As Microbiota Of Bivalve Molluscsmentioning

confidence: 99%

“…More recently, Beaz-Hidalgo et al (2008) analyzed the diversity of Vibrio spp. in cultured Manila clams ( Venerupis philippinarum ) and carpet-shell clams ( Venerupis decusata ) by means of phenotypic and genotypic methods.…”

Section: Vibrios As Microbiota Of Bivalve Molluscsmentioning

confidence: 99%

New Vibrio species associated to molluscan microbiota: a review

et al. 2014

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The genus Vibrio consists of more than 100 species grouped in 14 clades that are widely distributed in aquatic environments such as estuarine, coastal waters, and sediments. A large number of species of this genus are associated with marine organisms like fish, molluscs and crustaceans, in commensal or pathogenic relations. In the last decade, more than 50 new species have been described in the genus Vibrio, due to the introduction of new molecular techniques in bacterial taxonomy, such as multilocus sequence analysis or fluorescent amplified fragment length polymorphism. On the other hand, the increasing number of environmental studies has contributed to improve the knowledge about the family Vibrionaceae and its phylogeny. Vibrio crassostreae, V. breoganii, V. celticus are some of the new Vibrio species described as forming part of the molluscan microbiota. Some of them have been associated with mortalities of different molluscan species, seriously affecting their culture and causing high losses in hatcheries as well as in natural beds. For other species, ecological importance has been demonstrated being highly abundant in different marine habitats and geographical regions. The present work provides an updated overview of the recently characterized Vibrio species isolated from molluscs. In addition, their pathogenic potential and/or environmental importance is discussed.

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“…This species has become increasingly recognized as an important opportunistic pathogen causing epizootic outbreaks in cultured fish (Ben Kahla-Nakbi et al 2006), shellfish (Beaz Hidalgo et al 2008) and crustaceans (Gomez-Gil et al 1998). In our previous study, we found that V. alginolyticus species was the most commonly isolated (55 %) from the different compartments of R. decussatus hatchery (Mechri et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Castro et al (2002) characterized 123 Vibrio isolates from cultured Ruditapes philippinarum using phenotypic and genotypic methods and showed that V. harveyi, V. splendidus and V. tubiashii were the most abundant species. Moreover, Beaz Hidalgo et al (2008) reported that 67.7 % of the Vibrio species associated with cultured carpet shell clams (R. decussatus) and Manilla clams (R. philippinarum) belonged to V. splendidus, V. alginolyticus and V. diazotrophicus.…”

Section: Discussionmentioning

confidence: 99%

Impacts of rearing techniques on growth, survival and bacterial microbiota of carpet shell clam (Ruditapes decussatus) larvae

et al. 2016

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In this study, we investigate the impacts of two water treatment regimes, the closed aquaculture system (CAS) and flow-through system (FTS), on the carpet shell clam (Ruditapes decussatus) larval microbiota, growth and survival. A bacteriological barrier (B) was created among breeder stage treated by florfenicol (FF, 8 mg L -1 day -1 per 5 days). After spawning, larvae were maintained in five different rearing techniques: CAS; FTS; B ? CAS; B ? FTS and B ? CAS ? FF (8 mg L -1 day -1 per 3 days). Significant decrease was observed in both total bacterial count and presumptive Vibrio count (PVC) in FF-treated breeders. Besides, larvae reared in B ? FTS showed significantly lower PVC than larvae maintained in other rearing techniques. It was also determined that growth and survival of larvae reared in B ? CAS ? FF and B ? FTS were significantly better than those reared in other systems. One hundred and twelve Vibrio strains, isolated from breeders, eggs and larvae, were subjected to some bacteriological tests (API 20E strips, vibriostatic agent O/129, growth at different temperatures and salinity). Vibrio alginolyticus was the predominant isolated species (36.61 %), followed by V. fluvialis (25 %), V. vunificus (17.86 %), V. cholera (13.39 %) and V. paraheamolyticus (7.14 %). These results may contribute to the understanding of the effect of rearing techniques on the bacterial load, survival and growth of early life stages of R. decussatus larvae.

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Diversity of Vibrios associated with reared clams in Galicia (NW Spain)

Cited by 49 publications

References 28 publications

Occurrence, seasonality and infectivity of Vibrio strains in natural populations of mussels Mytilus galloprovincialis

Occurrence, seasonality and infectivity of Vibrio strains in natural populations of mussels Mytilus galloprovincialis

New Vibrio species associated to molluscan microbiota: a review

Impacts of rearing techniques on growth, survival and bacterial microbiota of carpet shell clam (Ruditapes decussatus) larvae

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