Diversity of zoonotic enterohepatic Helicobacter species and detection of a putative novel gastric Helicobacter species in wild and wild-born captive chimpanzees and western lowland gorillas

Flahou, Bram; Modrý, David; Pomajbíková, Kateřina; Petrželková, Klára J.; Smet, Annemieke; Ducatelle, Richard; Pasmans, Frank; Sá, Rui M.; Todd, Angelique; Hashimoto, Chie; Mulama, Martin; Kiang, John; Rossi, Mirko; Haesebrouck, Freddy

doi:10.1016/j.vetmic.2014.08.032

Cited by 15 publications

(8 citation statements)

References 40 publications

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“…Finally, the worst primers that appeared in our study were clearly demonstrated in BFHpyl and Heid which have the lowest significance in Insilco and laboratory evaluation ( P- 1.00) and low AUC (0.513, 0.450, respectively) by ROC analysis (Table 5) (Fig 3, 4). These results disagree with Flahou et al, and Farshad [59, 62].…”

Section: Discussioncontrasting

confidence: 99%

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Design of novel specific primers for helicobacter detection with diagnostic utility evaluation of different 16srRNA genus-specific primers: A comparative study

Abdelmalek

Shokry

Hamed

et al. 2021

Preprint

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Molecular diagnosis of helicobacters by PCR is simpler, more accurate, and feasible compared to other diagnostic methods. Validity and accuracy are highly dependent on the PCR primer design, diffusion time, and mutation rate of helicobacters. This study aimed to design 16srRNA -specific primers for Helicobacter spp. and H. pylori. Application of comparative statistical analysis of the diagnostic utility of the most available 16srRNA genus-specific primers. The new primers were designed using bioinformatics tools (MAFFT MSA and Gblocks command line). A comparative study was applied on nine genus-specific 16srRNA primers in comparison to the ConsH using Insilco and laboratory evaluation. The results demonstrated that the best specificity and sensitivity of the primers designed for this study compared to other primers. The comparative study revealed that the heminested outer/inner primers were the worst. Although H276,16srRNA(a), HeliS/Heli-nest, and Hcom had acceptable diagnostic utility, false positive and false negative results were obtained. Specificity testing on clinical samples indicated a surprising result; that H. pylori was not the sole enemy that we were looking for, but the NPH should be considered as a real risk prognostic for gastric diseases, consequently, a specific diagnosis and treatment should be developed. This study concluded that our designed primers were the most specific and sensitive in comparison with other primers. in addition, Insilco evaluation is not accurate enough for primer assessment and that the laboratory evaluation is mandatory.

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Section: Discussioncontrasting

confidence: 99%

“…However, the results of the current study are incompatible with Flahou et al, that Hcom1 is suitable for the identification of Helicobacter spp. [59], as it may give false-positive results with K. pneumonea . H276 may give positive results with S. mutans found in the buccal cavity [60].…”

Section: Discussionmentioning

confidence: 99%

Design of novel specific primers for helicobacter detection with diagnostic utility evaluation of different 16srRNA genus-specific primers: A comparative study

Abdelmalek

Shokry

Hamed

et al. 2021

Preprint

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“…One noteworthy finding from this microbiome research has been the extent to which captivity humanizes the microbiome of numerous primate species housed at a variety of institutions, including sanctuaries [248]. Researchers have also studied various bacteria using samples from chimpanzees at several African sanctuaries [221,[249][250][251][252], as well as biomarkers [253] and blood groups [254] in sanctuaryhoused great apes. One case study even describes blood transfusions to treat an anaemic chimpanzee at Tchimpounga [255].…”

Section: (C) Veterinary Genetic and Physiologymentioning

confidence: 99%

A review of research in primate sanctuaries

Ross

Leinwand

2020

Biol. Lett.

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While non-human primate studies have long been conducted in laboratories, and more recently at zoological parks, sanctuaries are increasingly considered a viable setting for research. Accredited sanctuaries in non-range countries house thousands of primates formerly used as subjects of medical research, trained performers or personal pets. In range countries, however, sanctuaries typically house orphaned primates confiscated from illegal poaching and the bushmeat and pet trafficking trades. Although the primary mission of these sanctuaries is to rescue and rehabilitate residents, many of these organizations are increasingly willing to participate in non-invasive research. Notably, from a scientific standpoint, most sanctuaries provide potential advantages over traditional settings, such as large, naturalistic physical and social environments which may result in more relevant models of primates' free-ranging wild counterparts than other captive settings. As a result, an impressive scope of research in the fields of primate behaviour, cognition, veterinary science, genetics and physiology have been studied in sanctuaries. In this review, we examine the range and form of research that has been conducted at accredited sanctuaries around the world. We also describe the potential challenges of sanctuary-based work and the considerations that external researchers may face when deciding to collaborate with primate sanctuaries on their research projects.

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“…By analyzing fecal samples, the presence of enterohepatic and gastric Helicobacter species was assessed in wild endangered chimpanzees and critically endangered western lowland gorillas from different African National parks and wild‐born captive animals from sanctuaries . Intact Helicobacter bacteria could be demonstrated, whereas cloning and sequencing of PCR‐amplified 16S rRNA gene fragments revealed a high homology to sequences from various zoonotic enterohepatic Helicobacter species, such as H. cinaedi and H. canadensis .…”

Section: Natural Infection With Non‐h Pylori Helicobacters In Animalsmentioning

confidence: 99%

The Other Helicobacters

et al. 2015

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In the past year, a substantial number of (putative) novel Helicobacter species have been described, including Helicobacter himalayensis colonizing the Himalayan marmot and Helicobacter apodemus, colonizing the Korean striped field mouse. In addition, a putative novel gastric Helicobacter species was identified in wild gorillas and chimpanzees, for which the name "Candidatus H. homininae" was proposed. A high incidence of gastric non-H. pylori Helicobacter infection was described in China and multiple case reports have described the involvement of enterohepatic Helicobacter species, especially Helicobacter cinaedi, in a wide range of diseases. Several studies in rodent models further elucidated the mechanisms underlying the development of gastric mucosaassociated lymphoid tissue lymphoma during infection with gastric non-H. pylori Helicobacters. The effects of infection with gastric Helicobacters on the development of neuroinflammation were investigated and several enterohepatic Helicobacter species were shown to affect the composition of the gut microbiota, to influence vaccine efficiency as well as the progression of cancer in distant sites of the body. Taxonomy and PhylogenyHelicobacter himalayensis was described as a new enterohepatic Helicobacter species in 2015 [1]. H. himalayensis was isolated from gastric mucosa of Marmota himalayana in the Qinghai-Tibet Plateau in China. Analysis of the 16S rRNA gene sequence (1468 bp) showed that it was most closely related to Helicobacter marmotae (96.7% similarity), while the 23S rRNA gene sequence (2879 bp) analysis showed that it was most closely related to Helicobacter canis (96% similarity).A novel Helicobacter species was identified from the gastrointestinal tract of Korean striped field mice, for which the name Helicobacter apodemus sp. nov. was proposed [2]. The isolates were positive for urease activity and were most closely related to a reference strain of Helicobacter mesocricetorum (97.03-97.32% 16S rRNA sequence similarities).A novel Helicobacter sp. was also isolated from the cecum of the Siberian hamster [3]. Sequence analysis showed 97% sequence similarity to Helicobacter ganmani. Coinfection with Helicobacter bilis and Campylobacter spp. was observed in the Siberian hamster. Genomics and GeneticsHelicobacter suis is one of the most fastidious Helicobacter species, requiring biphasic culture conditions. Hence, cultures obtained from an individual animal may contain multiple H. suis strains. A method was developed to obtain single-colony-derived H. suis strains by culturing bacteria as colonies on 1% brucella agar plates, followed by purification and enrichment by biphasic culture [4]. However, for four of 17 strains, MLST analysis revealed genetic differences between parental and single-colony-derived strains. One colony-derived strain was further shown to have lost its capacity to colonize Mongolian gerbils.Draft genome sequences of seven enterohepatic Helicobacter species were described [5]. Six isolates (H. bilis ATCC 43879, H. canis NCTC 12...

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Diversity of zoonotic enterohepatic Helicobacter species and detection of a putative novel gastric Helicobacter species in wild and wild-born captive chimpanzees and western lowland gorillas

Cited by 15 publications

References 40 publications

Design of novel specific primers for helicobacter detection with diagnostic utility evaluation of different 16srRNA genus-specific primers: A comparative study

Design of novel specific primers for helicobacter detection with diagnostic utility evaluation of different 16srRNA genus-specific primers: A comparative study

A review of research in primate sanctuaries

The Other Helicobacters

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